Diesel-born organosulfur compounds stimulate community re-structuring in a diesel-biodesulfurizing consortium

Maysoon Awadh, Huda Mahmoud, Raeid M.M. Abed, Ashraf M. El Nayal, Nasser Abotalib, Wael Ismail*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

We enriched and characterized a biodesulfurizing consortium (designated as MG1). The MG1 consortium reduced the total sulfur of diesel by 25 % and utilized each of the diesel-born compounds dibenzothiophene (DBT), benzothiophene (BT), 4-methyldibenzothiophene (4-MDBT) and 4, 6-dimethyldibenzothiophene (4, 6-DMDBT) as a sole sulfur source. MiSeq analysis revealed compositional shifts in the MG1 community according to the type of the sulfur source. A DBT-grown MG1 culture had Klebsiella, Pseudomonas, Rhodococcus and Sphingomonas as the most abundant genera. When diesel or 4, 6-DMDBT was provided as a sole sulfur source, Klebsiella and Pseudomonas spp. were the most abundant. In the BT culture, Rhodococcus spp. were the key biodesulfurizers, while Klebsiella, Pseudomonas and Sphingomonas spp. dominated the 4-MDBT-grown consortium. MG1 also utilized 2–hydroxybiphenyl (the product of the 4S biodesulfurization pathway) where Pseudomonas spp. uniquely dominated the consortium. The data improves our understanding of the sulfur source-driven structural adaptability of biodesulfurizing consortia.

Original languageEnglish
Article numbere00572
JournalBiotechnology Reports
Volume28
DOIs
Publication statusPublished - Dec 2020
Externally publishedYes

Keywords

  • 4S pathway
  • Biodesulfurization
  • Dibenzothiophene
  • Diesel
  • Microbial consortia
  • Rhodococcus

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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