Diagnostic Mr31/32 000 Schistosoma mansoni proteins (Sm31/32)

Reaction with sera from Sudanese patients infected with S. mansoni or S. haematobium

M. A. Idris, A. Ruppel

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25 Citations (Scopus)

Abstract

Sera of Sudanese patients with active infections of Schistosoma mansoni or S. haematobium were tested in immunoblots for their reactivity with Mr31/32 000 proteins of adult S. mansoni (Sm31/32). All sera from patients with intestinal (n = 123) and all but one from those with urinary schistosomiasis (n = 35) had antibodies against Sm31/32. These and additional data suggest that both specificity and sensitivity of Sm31/32 to detect schistosome infections are close to 100%. Antibodies against these proteins developed also in monkeys after experimental infection with S. haematobium. Sm31/32 antigens reacted in immunoblots as a doublet with most S. haematobium-patient sera and as a broad band with many S. mansoni-sera suggesting that at least two components are present in the molecular weight region of Mr31/32 000. The data demonstrate the potential use of Sm31/32 from adult worms to diagnose patients with intestinal or urinary schistosomiasis in endemic areas.

Original languageEnglish
Pages (from-to)95-101
Number of pages7
JournalJournal of Helminthology
Volume62
Issue number2
Publication statusPublished - 1988

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Schistosoma haematobium
Schistosoma mansoni
Haematobia
Schistosomiasis haematobia
Schistosomiasis mansoni
Serum
proteins
infection
antibodies
Schistosoma
Antibodies
Infection
Haplorhini
monkeys
Molecular Weight
molecular weight
antigens
Antigens
Sensitivity and Specificity
Schistosoma mansoni Sm31 protein

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Parasitology

Cite this

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title = "Diagnostic Mr31/32 000 Schistosoma mansoni proteins (Sm31/32): Reaction with sera from Sudanese patients infected with S. mansoni or S. haematobium",
abstract = "Sera of Sudanese patients with active infections of Schistosoma mansoni or S. haematobium were tested in immunoblots for their reactivity with Mr31/32 000 proteins of adult S. mansoni (Sm31/32). All sera from patients with intestinal (n = 123) and all but one from those with urinary schistosomiasis (n = 35) had antibodies against Sm31/32. These and additional data suggest that both specificity and sensitivity of Sm31/32 to detect schistosome infections are close to 100{\%}. Antibodies against these proteins developed also in monkeys after experimental infection with S. haematobium. Sm31/32 antigens reacted in immunoblots as a doublet with most S. haematobium-patient sera and as a broad band with many S. mansoni-sera suggesting that at least two components are present in the molecular weight region of Mr31/32 000. The data demonstrate the potential use of Sm31/32 from adult worms to diagnose patients with intestinal or urinary schistosomiasis in endemic areas.",
author = "Idris, {M. A.} and A. Ruppel",
year = "1988",
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journal = "Journal of Helminthology",
issn = "0022-149X",
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T1 - Diagnostic Mr31/32 000 Schistosoma mansoni proteins (Sm31/32)

T2 - Reaction with sera from Sudanese patients infected with S. mansoni or S. haematobium

AU - Idris, M. A.

AU - Ruppel, A.

PY - 1988

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N2 - Sera of Sudanese patients with active infections of Schistosoma mansoni or S. haematobium were tested in immunoblots for their reactivity with Mr31/32 000 proteins of adult S. mansoni (Sm31/32). All sera from patients with intestinal (n = 123) and all but one from those with urinary schistosomiasis (n = 35) had antibodies against Sm31/32. These and additional data suggest that both specificity and sensitivity of Sm31/32 to detect schistosome infections are close to 100%. Antibodies against these proteins developed also in monkeys after experimental infection with S. haematobium. Sm31/32 antigens reacted in immunoblots as a doublet with most S. haematobium-patient sera and as a broad band with many S. mansoni-sera suggesting that at least two components are present in the molecular weight region of Mr31/32 000. The data demonstrate the potential use of Sm31/32 from adult worms to diagnose patients with intestinal or urinary schistosomiasis in endemic areas.

AB - Sera of Sudanese patients with active infections of Schistosoma mansoni or S. haematobium were tested in immunoblots for their reactivity with Mr31/32 000 proteins of adult S. mansoni (Sm31/32). All sera from patients with intestinal (n = 123) and all but one from those with urinary schistosomiasis (n = 35) had antibodies against Sm31/32. These and additional data suggest that both specificity and sensitivity of Sm31/32 to detect schistosome infections are close to 100%. Antibodies against these proteins developed also in monkeys after experimental infection with S. haematobium. Sm31/32 antigens reacted in immunoblots as a doublet with most S. haematobium-patient sera and as a broad band with many S. mansoni-sera suggesting that at least two components are present in the molecular weight region of Mr31/32 000. The data demonstrate the potential use of Sm31/32 from adult worms to diagnose patients with intestinal or urinary schistosomiasis in endemic areas.

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