Diagnostic Mr 31/32000 protein of Schistosoma mansoni (Sm31/32) and S. haematobium (Sh31/32)

Stability and reaction conditions for prospective field tests

M. A. Idris, A. Ruppel

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The existence of adult Schistosoma haematobium worm proteins (Sh31/32) homologous to the diagnostic Sm31/32 of S. mansoni is shown. Sm31/32 and Sh31/32, adsorbed on nitrocellulose and kept dry on the bench before immunoblot analysis, were antigenically stable for at least 4 years including storage for 17 months in tropical climates. The antigens react with patient sera in the absence of defined buffers under rather simple conditions ('humid chamber blot'). The results add to the use of these antigens for serodiagnosis of schistosomiasis in endemic areas.

Original languageEnglish
Pages (from-to)89-94
Number of pages6
JournalJournal of Helminthology
Volume65
Issue number2
Publication statusPublished - 1991

Fingerprint

Schistosoma haematobium
Schistosoma mansoni
Tropical Climate
antigens
Antigens
schistosomiasis
Collodion
serodiagnosis
Schistosomiasis
Serologic Tests
tropics
Buffers
buffers
proteins
testing
Serum
Proteins
Schistosoma mansoni Sm31 protein

ASJC Scopus subject areas

  • Parasitology
  • Animal Science and Zoology

Cite this

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abstract = "The existence of adult Schistosoma haematobium worm proteins (Sh31/32) homologous to the diagnostic Sm31/32 of S. mansoni is shown. Sm31/32 and Sh31/32, adsorbed on nitrocellulose and kept dry on the bench before immunoblot analysis, were antigenically stable for at least 4 years including storage for 17 months in tropical climates. The antigens react with patient sera in the absence of defined buffers under rather simple conditions ('humid chamber blot'). The results add to the use of these antigens for serodiagnosis of schistosomiasis in endemic areas.",
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AU - Idris, M. A.

AU - Ruppel, A.

PY - 1991

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AB - The existence of adult Schistosoma haematobium worm proteins (Sh31/32) homologous to the diagnostic Sm31/32 of S. mansoni is shown. Sm31/32 and Sh31/32, adsorbed on nitrocellulose and kept dry on the bench before immunoblot analysis, were antigenically stable for at least 4 years including storage for 17 months in tropical climates. The antigens react with patient sera in the absence of defined buffers under rather simple conditions ('humid chamber blot'). The results add to the use of these antigens for serodiagnosis of schistosomiasis in endemic areas.

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