Development of wheat scab symptoms is delayed in transgenic wheat plants that constitutively express a rice thaumatin-like protein gene

W. P. Chen, P. D. Chen, D. J. Liu, R. Kynast, B. Friebe, R. Velazhahan, S. Muthukrishnan, B. S. Gill

Research output: Contribution to journalArticle

155 Citations (Scopus)

Abstract

The possibility of controlling wheat scab (caused by Fusarium graminearum Schw.) was explored by engineering wheat plants for constitutive expression of pathogenesis-related (PR) protein genes. A rice thaumatin-like protein (TLP) gene (tlp) and a rice chitinase gene (chi11) were introduced into the spring wheat cultivar 'Bobwhite' by co-transformation of the plasmids pGL2ubi-tlp (ubiquitin/tlp//CaMV 35S/hpt) and pAHG11 (CaMV 35S/chi11//ubiquitin/bar). The transformation was by biolistic bombardment. Bialaphos was used as the selection reagent. The integration and expression of the tlp, bar, chi11 and hpt genes were analyzed by Southern, Northern and Western blot analyses. The four transgenes co-segregated in the T1 progeny of the transgenic plant and were localized at the telomeric region of the chromosome 6A long arm by sequential N-banding and fluorescent in situ hybridization (FISH) using pAHG11 or pGL2ubi-tlp as the probes. Only the transgenes tlp and bar, under the control of the ubiquitin promoter-intron, were expressed. No expression of the chi11 and hpt genes, controlled by the CaMV 35S promoter, was detected in T1 plants. After inoculation with conidia of F. graminearum, the symptoms of scab developed significantly slower in transgenic plants of the T1, T2 and T3 generations expressing the tlp gene than in non-transformed control plants. This is the first report of enhanced resistance to F. graminearum in transgenic wheat plants with constitutive expression of TLP.

Original languageEnglish
Pages (from-to)755-760
Number of pages6
JournalTheoretical And Applied Genetics
Volume99
Issue number5
DOIs
Publication statusPublished - 1999

Fingerprint

Genetically Modified Plants
signs and symptoms (plants)
Triticum
Ubiquitin
genetically modified organisms
rice
wheat
Fusarium graminearum
ubiquitin
Transgenes
Genes
Proteins
Colinus
genes
proteins
Biolistics
Chitinases
transgenes
Fungal Spores
transgenic plants

Keywords

  • Fluorescent in situ hybridization
  • Genetic transformation
  • Thaumatin-like protein
  • Triticum aestivum
  • Wheat scab

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Horticulture
  • Plant Science
  • Genetics
  • Genetics(clinical)

Cite this

Development of wheat scab symptoms is delayed in transgenic wheat plants that constitutively express a rice thaumatin-like protein gene. / Chen, W. P.; Chen, P. D.; Liu, D. J.; Kynast, R.; Friebe, B.; Velazhahan, R.; Muthukrishnan, S.; Gill, B. S.

In: Theoretical And Applied Genetics, Vol. 99, No. 5, 1999, p. 755-760.

Research output: Contribution to journalArticle

Chen, W. P. ; Chen, P. D. ; Liu, D. J. ; Kynast, R. ; Friebe, B. ; Velazhahan, R. ; Muthukrishnan, S. ; Gill, B. S. / Development of wheat scab symptoms is delayed in transgenic wheat plants that constitutively express a rice thaumatin-like protein gene. In: Theoretical And Applied Genetics. 1999 ; Vol. 99, No. 5. pp. 755-760.
@article{340aceeb04a64a5182aa2019510d6d86,
title = "Development of wheat scab symptoms is delayed in transgenic wheat plants that constitutively express a rice thaumatin-like protein gene",
abstract = "The possibility of controlling wheat scab (caused by Fusarium graminearum Schw.) was explored by engineering wheat plants for constitutive expression of pathogenesis-related (PR) protein genes. A rice thaumatin-like protein (TLP) gene (tlp) and a rice chitinase gene (chi11) were introduced into the spring wheat cultivar 'Bobwhite' by co-transformation of the plasmids pGL2ubi-tlp (ubiquitin/tlp//CaMV 35S/hpt) and pAHG11 (CaMV 35S/chi11//ubiquitin/bar). The transformation was by biolistic bombardment. Bialaphos was used as the selection reagent. The integration and expression of the tlp, bar, chi11 and hpt genes were analyzed by Southern, Northern and Western blot analyses. The four transgenes co-segregated in the T1 progeny of the transgenic plant and were localized at the telomeric region of the chromosome 6A long arm by sequential N-banding and fluorescent in situ hybridization (FISH) using pAHG11 or pGL2ubi-tlp as the probes. Only the transgenes tlp and bar, under the control of the ubiquitin promoter-intron, were expressed. No expression of the chi11 and hpt genes, controlled by the CaMV 35S promoter, was detected in T1 plants. After inoculation with conidia of F. graminearum, the symptoms of scab developed significantly slower in transgenic plants of the T1, T2 and T3 generations expressing the tlp gene than in non-transformed control plants. This is the first report of enhanced resistance to F. graminearum in transgenic wheat plants with constitutive expression of TLP.",
keywords = "Fluorescent in situ hybridization, Genetic transformation, Thaumatin-like protein, Triticum aestivum, Wheat scab",
author = "Chen, {W. P.} and Chen, {P. D.} and Liu, {D. J.} and R. Kynast and B. Friebe and R. Velazhahan and S. Muthukrishnan and Gill, {B. S.}",
year = "1999",
doi = "10.1007/s001220051294",
language = "English",
volume = "99",
pages = "755--760",
journal = "Theoretical And Applied Genetics",
issn = "0040-5752",
publisher = "Springer Verlag",
number = "5",

}

TY - JOUR

T1 - Development of wheat scab symptoms is delayed in transgenic wheat plants that constitutively express a rice thaumatin-like protein gene

AU - Chen, W. P.

AU - Chen, P. D.

AU - Liu, D. J.

AU - Kynast, R.

AU - Friebe, B.

AU - Velazhahan, R.

AU - Muthukrishnan, S.

AU - Gill, B. S.

PY - 1999

Y1 - 1999

N2 - The possibility of controlling wheat scab (caused by Fusarium graminearum Schw.) was explored by engineering wheat plants for constitutive expression of pathogenesis-related (PR) protein genes. A rice thaumatin-like protein (TLP) gene (tlp) and a rice chitinase gene (chi11) were introduced into the spring wheat cultivar 'Bobwhite' by co-transformation of the plasmids pGL2ubi-tlp (ubiquitin/tlp//CaMV 35S/hpt) and pAHG11 (CaMV 35S/chi11//ubiquitin/bar). The transformation was by biolistic bombardment. Bialaphos was used as the selection reagent. The integration and expression of the tlp, bar, chi11 and hpt genes were analyzed by Southern, Northern and Western blot analyses. The four transgenes co-segregated in the T1 progeny of the transgenic plant and were localized at the telomeric region of the chromosome 6A long arm by sequential N-banding and fluorescent in situ hybridization (FISH) using pAHG11 or pGL2ubi-tlp as the probes. Only the transgenes tlp and bar, under the control of the ubiquitin promoter-intron, were expressed. No expression of the chi11 and hpt genes, controlled by the CaMV 35S promoter, was detected in T1 plants. After inoculation with conidia of F. graminearum, the symptoms of scab developed significantly slower in transgenic plants of the T1, T2 and T3 generations expressing the tlp gene than in non-transformed control plants. This is the first report of enhanced resistance to F. graminearum in transgenic wheat plants with constitutive expression of TLP.

AB - The possibility of controlling wheat scab (caused by Fusarium graminearum Schw.) was explored by engineering wheat plants for constitutive expression of pathogenesis-related (PR) protein genes. A rice thaumatin-like protein (TLP) gene (tlp) and a rice chitinase gene (chi11) were introduced into the spring wheat cultivar 'Bobwhite' by co-transformation of the plasmids pGL2ubi-tlp (ubiquitin/tlp//CaMV 35S/hpt) and pAHG11 (CaMV 35S/chi11//ubiquitin/bar). The transformation was by biolistic bombardment. Bialaphos was used as the selection reagent. The integration and expression of the tlp, bar, chi11 and hpt genes were analyzed by Southern, Northern and Western blot analyses. The four transgenes co-segregated in the T1 progeny of the transgenic plant and were localized at the telomeric region of the chromosome 6A long arm by sequential N-banding and fluorescent in situ hybridization (FISH) using pAHG11 or pGL2ubi-tlp as the probes. Only the transgenes tlp and bar, under the control of the ubiquitin promoter-intron, were expressed. No expression of the chi11 and hpt genes, controlled by the CaMV 35S promoter, was detected in T1 plants. After inoculation with conidia of F. graminearum, the symptoms of scab developed significantly slower in transgenic plants of the T1, T2 and T3 generations expressing the tlp gene than in non-transformed control plants. This is the first report of enhanced resistance to F. graminearum in transgenic wheat plants with constitutive expression of TLP.

KW - Fluorescent in situ hybridization

KW - Genetic transformation

KW - Thaumatin-like protein

KW - Triticum aestivum

KW - Wheat scab

UR - http://www.scopus.com/inward/record.url?scp=0032877906&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032877906&partnerID=8YFLogxK

U2 - 10.1007/s001220051294

DO - 10.1007/s001220051294

M3 - Article

VL - 99

SP - 755

EP - 760

JO - Theoretical And Applied Genetics

JF - Theoretical And Applied Genetics

SN - 0040-5752

IS - 5

ER -