Abstract
Fifty-four primer pairs were designed for expressed sequence tag (EST) sequences containing perfect di- and tri-nucleotide motifs and characterised in 96 unrelated fish. Twenty markers were successfully amplified with number of alleles from 2 to 10 per locus and observed and expected heterozygosity ranging from 0.01 to 0.56 and 0.03 to 0.70, respectively. Loci Gmo-C213, Gmo-C246 and Gmo-C247 deviated from Hardy-Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed significant departure from the null hypothesis between loci Gmo-C213 and Gmo-C222, Gmo-C233 and Gmo-C229, C223 and Gmo-C236 and C229 and Gmo-C236. The gene identity was determined at 10 of the loci, confirming the associated microsatellites as Type I markers. These microsatellite markers provide useful tools for studies of population genetics, reproductive ecology and constructing linkage maps of Atlantic cod.
| Original language | English |
|---|---|
| Pages (from-to) | 1395-1398 |
| Number of pages | 4 |
| Journal | Conservation Genetics |
| Volume | 9 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Oct 2008 |
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Keywords
- Atlantic cod
- cDNA
- Expressed sequence tags (ESTs)
- Gadus morhua
- Microsatellites
- Type I markers
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Genetics
- Genetics(clinical)
- Nature and Landscape Conservation
Cite this
Development of twenty sequence-tagged microsatellites for the Atlantic cod (Gadus morhua L.). / Delghandi, Madjid; Wesmajervi, Mette Serine; Mennen, Saskia; Nilsen, Frank.
In: Conservation Genetics, Vol. 9, No. 5, 10.2008, p. 1395-1398.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Development of twenty sequence-tagged microsatellites for the Atlantic cod (Gadus morhua L.)
AU - Delghandi, Madjid
AU - Wesmajervi, Mette Serine
AU - Mennen, Saskia
AU - Nilsen, Frank
PY - 2008/10
Y1 - 2008/10
N2 - Fifty-four primer pairs were designed for expressed sequence tag (EST) sequences containing perfect di- and tri-nucleotide motifs and characterised in 96 unrelated fish. Twenty markers were successfully amplified with number of alleles from 2 to 10 per locus and observed and expected heterozygosity ranging from 0.01 to 0.56 and 0.03 to 0.70, respectively. Loci Gmo-C213, Gmo-C246 and Gmo-C247 deviated from Hardy-Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed significant departure from the null hypothesis between loci Gmo-C213 and Gmo-C222, Gmo-C233 and Gmo-C229, C223 and Gmo-C236 and C229 and Gmo-C236. The gene identity was determined at 10 of the loci, confirming the associated microsatellites as Type I markers. These microsatellite markers provide useful tools for studies of population genetics, reproductive ecology and constructing linkage maps of Atlantic cod.
AB - Fifty-four primer pairs were designed for expressed sequence tag (EST) sequences containing perfect di- and tri-nucleotide motifs and characterised in 96 unrelated fish. Twenty markers were successfully amplified with number of alleles from 2 to 10 per locus and observed and expected heterozygosity ranging from 0.01 to 0.56 and 0.03 to 0.70, respectively. Loci Gmo-C213, Gmo-C246 and Gmo-C247 deviated from Hardy-Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed significant departure from the null hypothesis between loci Gmo-C213 and Gmo-C222, Gmo-C233 and Gmo-C229, C223 and Gmo-C236 and C229 and Gmo-C236. The gene identity was determined at 10 of the loci, confirming the associated microsatellites as Type I markers. These microsatellite markers provide useful tools for studies of population genetics, reproductive ecology and constructing linkage maps of Atlantic cod.
KW - Atlantic cod
KW - cDNA
KW - Expressed sequence tags (ESTs)
KW - Gadus morhua
KW - Microsatellites
KW - Type I markers
UR - http://www.scopus.com/inward/record.url?scp=50249184087&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=50249184087&partnerID=8YFLogxK
U2 - 10.1007/s10592-008-9507-3
DO - 10.1007/s10592-008-9507-3
M3 - Article
VL - 9
SP - 1395
EP - 1398
JO - Conservation Genetics
JF - Conservation Genetics
SN - 1566-0621
IS - 5
ER -