Development of twenty sequence-tagged microsatellites for the Atlantic cod (Gadus morhua L.)

Madjid Delghandi, Mette Serine Wesmajervi, Saskia Mennen, Frank Nilsen

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Fifty-four primer pairs were designed for expressed sequence tag (EST) sequences containing perfect di- and tri-nucleotide motifs and characterised in 96 unrelated fish. Twenty markers were successfully amplified with number of alleles from 2 to 10 per locus and observed and expected heterozygosity ranging from 0.01 to 0.56 and 0.03 to 0.70, respectively. Loci Gmo-C213, Gmo-C246 and Gmo-C247 deviated from Hardy-Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed significant departure from the null hypothesis between loci Gmo-C213 and Gmo-C222, Gmo-C233 and Gmo-C229, C223 and Gmo-C236 and C229 and Gmo-C236. The gene identity was determined at 10 of the loci, confirming the associated microsatellites as Type I markers. These microsatellite markers provide useful tools for studies of population genetics, reproductive ecology and constructing linkage maps of Atlantic cod.

Original languageEnglish
Pages (from-to)1395-1398
Number of pages4
JournalConservation Genetics
Volume9
Issue number5
DOIs
Publication statusPublished - Oct 2008

Fingerprint

Gadus morhua
Microsatellite Repeats
microsatellite repeats
Nucleotide Motifs
loci
Genetic Linkage
Expressed Sequence Tags
Linkage Disequilibrium
Population Genetics
Ecology
Fishes
Nucleotides
Alleles
disequilibrium
heterozygosity
population genetics
allele
Genes
ecology
linkage disequilibrium

Keywords

  • Atlantic cod
  • cDNA
  • Expressed sequence tags (ESTs)
  • Gadus morhua
  • Microsatellites
  • Type I markers

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Genetics
  • Genetics(clinical)
  • Nature and Landscape Conservation

Cite this

Development of twenty sequence-tagged microsatellites for the Atlantic cod (Gadus morhua L.). / Delghandi, Madjid; Wesmajervi, Mette Serine; Mennen, Saskia; Nilsen, Frank.

In: Conservation Genetics, Vol. 9, No. 5, 10.2008, p. 1395-1398.

Research output: Contribution to journalArticle

Delghandi, Madjid ; Wesmajervi, Mette Serine ; Mennen, Saskia ; Nilsen, Frank. / Development of twenty sequence-tagged microsatellites for the Atlantic cod (Gadus morhua L.). In: Conservation Genetics. 2008 ; Vol. 9, No. 5. pp. 1395-1398.
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