TY - JOUR
T1 - Development of twenty sequence-tagged microsatellites for the Atlantic cod (Gadus morhua L.)
AU - Delghandi, Madjid
AU - Wesmajervi, Mette Serine
AU - Mennen, Saskia
AU - Nilsen, Frank
N1 - Funding Information:
Acknowledgements The present study was supported by the National Programme for Research in Functional Genomics in Norway (FUGE) within the Research Council of Norway (GenoFish). We thank Dr. Marie Cooper for reading of the manuscript and making helpful suggestions.
PY - 2008/10
Y1 - 2008/10
N2 - Fifty-four primer pairs were designed for expressed sequence tag (EST) sequences containing perfect di- and tri-nucleotide motifs and characterised in 96 unrelated fish. Twenty markers were successfully amplified with number of alleles from 2 to 10 per locus and observed and expected heterozygosity ranging from 0.01 to 0.56 and 0.03 to 0.70, respectively. Loci Gmo-C213, Gmo-C246 and Gmo-C247 deviated from Hardy-Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed significant departure from the null hypothesis between loci Gmo-C213 and Gmo-C222, Gmo-C233 and Gmo-C229, C223 and Gmo-C236 and C229 and Gmo-C236. The gene identity was determined at 10 of the loci, confirming the associated microsatellites as Type I markers. These microsatellite markers provide useful tools for studies of population genetics, reproductive ecology and constructing linkage maps of Atlantic cod.
AB - Fifty-four primer pairs were designed for expressed sequence tag (EST) sequences containing perfect di- and tri-nucleotide motifs and characterised in 96 unrelated fish. Twenty markers were successfully amplified with number of alleles from 2 to 10 per locus and observed and expected heterozygosity ranging from 0.01 to 0.56 and 0.03 to 0.70, respectively. Loci Gmo-C213, Gmo-C246 and Gmo-C247 deviated from Hardy-Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed significant departure from the null hypothesis between loci Gmo-C213 and Gmo-C222, Gmo-C233 and Gmo-C229, C223 and Gmo-C236 and C229 and Gmo-C236. The gene identity was determined at 10 of the loci, confirming the associated microsatellites as Type I markers. These microsatellite markers provide useful tools for studies of population genetics, reproductive ecology and constructing linkage maps of Atlantic cod.
KW - Atlantic cod
KW - Expressed sequence tags (ESTs)
KW - Gadus morhua
KW - Microsatellites
KW - Type I markers
KW - cDNA
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U2 - 10.1007/s10592-008-9507-3
DO - 10.1007/s10592-008-9507-3
M3 - Article
AN - SCOPUS:50249184087
SN - 1566-0621
VL - 9
SP - 1395
EP - 1398
JO - Conservation Genetics
JF - Conservation Genetics
IS - 5
ER -