Abstract
Fifty-four primer pairs were designed for expressed sequence tag (EST) sequences containing perfect di- and tri-nucleotide motifs and characterised in 96 unrelated fish. Twenty markers were successfully amplified with number of alleles from 2 to 10 per locus and observed and expected heterozygosity ranging from 0.01 to 0.56 and 0.03 to 0.70, respectively. Loci Gmo-C213, Gmo-C246 and Gmo-C247 deviated from Hardy-Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed significant departure from the null hypothesis between loci Gmo-C213 and Gmo-C222, Gmo-C233 and Gmo-C229, C223 and Gmo-C236 and C229 and Gmo-C236. The gene identity was determined at 10 of the loci, confirming the associated microsatellites as Type I markers. These microsatellite markers provide useful tools for studies of population genetics, reproductive ecology and constructing linkage maps of Atlantic cod.
Original language | English |
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Pages (from-to) | 1395-1398 |
Number of pages | 4 |
Journal | Conservation Genetics |
Volume | 9 |
Issue number | 5 |
DOIs | |
Publication status | Published - Oct 2008 |
Keywords
- Atlantic cod
- cDNA
- Expressed sequence tags (ESTs)
- Gadus morhua
- Microsatellites
- Type I markers
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Genetics
- Genetics(clinical)
- Nature and Landscape Conservation