Development of twenty sequence-tagged microsatellites for the Atlantic cod (Gadus morhua L.)

Madjid Delghandi, Mette Serine Wesmajervi, Saskia Mennen, Frank Nilsen

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4 Citations (Scopus)

Abstract

Fifty-four primer pairs were designed for expressed sequence tag (EST) sequences containing perfect di- and tri-nucleotide motifs and characterised in 96 unrelated fish. Twenty markers were successfully amplified with number of alleles from 2 to 10 per locus and observed and expected heterozygosity ranging from 0.01 to 0.56 and 0.03 to 0.70, respectively. Loci Gmo-C213, Gmo-C246 and Gmo-C247 deviated from Hardy-Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed significant departure from the null hypothesis between loci Gmo-C213 and Gmo-C222, Gmo-C233 and Gmo-C229, C223 and Gmo-C236 and C229 and Gmo-C236. The gene identity was determined at 10 of the loci, confirming the associated microsatellites as Type I markers. These microsatellite markers provide useful tools for studies of population genetics, reproductive ecology and constructing linkage maps of Atlantic cod.

Original languageEnglish
Pages (from-to)1395-1398
Number of pages4
JournalConservation Genetics
Volume9
Issue number5
DOIs
Publication statusPublished - Oct 2008

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Keywords

  • Atlantic cod
  • cDNA
  • Expressed sequence tags (ESTs)
  • Gadus morhua
  • Microsatellites
  • Type I markers

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Genetics
  • Genetics(clinical)
  • Nature and Landscape Conservation

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