Development of a computer software for analysis of SDS-PAGE protein fingerprints of bacterial isolates

S. K. Saxena, A. N A Ibrahim, Santanu Chaudhury, S. S. Thukral

Research output: Contribution to journalArticle

2 Citations (Scopus)


Protein fingerprinting is a widely used technique in epidemiological studies for typing bacterial strains. This study reports the development of a computer based gel analysis system. The system has the capability to analyse SDS-PAGE whole-cell protein profiles using digital image processing techniques. The software incorporates spatial and frequency domain operators for image enhancement, support for geometric correction of images and new algorithms for identification of strain tracks and protein bands. The system also provides facilities for correcting imaging defects for inter-gel comparison, similarity analysis, clustering and pictorial representation of results as a dendrogram. The software is highly interactive, user-friendly and can produce accurate results for differentiation of bacterial strains with minimal overhead of time.

Original languageEnglish
Pages (from-to)167-176
Number of pages10
JournalIndian Journal of Experimental Biology
Issue number2
Publication statusPublished - 2000


ASJC Scopus subject areas

  • Bioengineering

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