Development of a computer software for analysis of SDS-PAGE protein fingerprints of bacterial isolates

S. K. Saxena, A. N A Ibrahim, Santanu Chaudhury, S. S. Thukral

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Protein fingerprinting is a widely used technique in epidemiological studies for typing bacterial strains. This study reports the development of a computer based gel analysis system. The system has the capability to analyse SDS-PAGE whole-cell protein profiles using digital image processing techniques. The software incorporates spatial and frequency domain operators for image enhancement, support for geometric correction of images and new algorithms for identification of strain tracks and protein bands. The system also provides facilities for correcting imaging defects for inter-gel comparison, similarity analysis, clustering and pictorial representation of results as a dendrogram. The software is highly interactive, user-friendly and can produce accurate results for differentiation of bacterial strains with minimal overhead of time.

Original languageEnglish
Pages (from-to)167-176
Number of pages10
JournalIndian Journal of Experimental Biology
Volume38
Issue number2
Publication statusPublished - 2000

Fingerprint

Peptide Mapping
Bacterial Proteins
Polyacrylamide Gel Electrophoresis
Bacterial Typing Techniques
Software
Gels
Image Enhancement
Proteins
Cluster Analysis
Epidemiologic Studies
Image enhancement
Image processing
Imaging techniques
Defects

ASJC Scopus subject areas

  • Bioengineering

Cite this

Development of a computer software for analysis of SDS-PAGE protein fingerprints of bacterial isolates. / Saxena, S. K.; Ibrahim, A. N A; Chaudhury, Santanu; Thukral, S. S.

In: Indian Journal of Experimental Biology, Vol. 38, No. 2, 2000, p. 167-176.

Research output: Contribution to journalArticle

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