TY - JOUR
T1 - Development and comparison of ELISA and PCR methods for the early detection of Ganoderma disease of coconut
AU - Karthikeyan, M.
AU - Bhaskaran, R.
AU - Radhika, K.
AU - Mathiyazhagan, S.
AU - Jayakumar, V.
AU - Sandosskumar, R.
AU - Velazhahan, R.
N1 - Funding Information:
The financial support given by the Council of Scientific and Industrial New Delhi, India for conducting this study is gratefully acknowledged.
PY - 2008/9
Y1 - 2008/9
N2 - Molecular diagnosis, chemo-diagnosis and physiological parameter have been applied for detecting the Ganoderma disease of coconut. Polyclonal antibodies (PAbs) raised against mycelial protein of Ganoderma, specific mycelial protein (62 kDa) of Ganoderma isolates and basidiocarp protein of Ganoderma were used for detection. All the PAbs could detect Ganoderma in diseased coconut root tissues in early stage of the disease before symptom expression by indirect - ELISA at the antiserum dilution of 1:1000 for mycelial protein, 1:700 for specific protein and 1:3000 for basidiocarp protein. Low cross reactions were observed with saprophytic fungi occurring in coconut roots and also with other basidiomycetous fungi. For polymerase chain reaction tests, the primer was generated from the internal transcribed spacer region one (ITS 1) of rDNA of Ganoderma, which produced a PCR product of 167 bp in size. Utility of this method was confirmed at the field level.
AB - Molecular diagnosis, chemo-diagnosis and physiological parameter have been applied for detecting the Ganoderma disease of coconut. Polyclonal antibodies (PAbs) raised against mycelial protein of Ganoderma, specific mycelial protein (62 kDa) of Ganoderma isolates and basidiocarp protein of Ganoderma were used for detection. All the PAbs could detect Ganoderma in diseased coconut root tissues in early stage of the disease before symptom expression by indirect - ELISA at the antiserum dilution of 1:1000 for mycelial protein, 1:700 for specific protein and 1:3000 for basidiocarp protein. Low cross reactions were observed with saprophytic fungi occurring in coconut roots and also with other basidiomycetous fungi. For polymerase chain reaction tests, the primer was generated from the internal transcribed spacer region one (ITS 1) of rDNA of Ganoderma, which produced a PCR product of 167 bp in size. Utility of this method was confirmed at the field level.
KW - Basidiomycetous
KW - ELISA
KW - Ganoderma
KW - Internal transcribed spacer
KW - Polyclonal antibodies
KW - Polymerase chain reaction
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U2 - 10.1080/03235400600813417
DO - 10.1080/03235400600813417
M3 - Article
AN - SCOPUS:45849149683
SN - 0323-5408
VL - 41
SP - 396
EP - 406
JO - Archives of Phytopathology and Plant Protection
JF - Archives of Phytopathology and Plant Protection
IS - 6
ER -