Development and comparison of ELISA and PCR methods for the early detection of Ganoderma disease of coconut

M. Karthikeyan, R. Bhaskaran, K. Radhika, S. Mathiyazhagan, V. Jayakumar, R. Sandosskumar, R. Velazhahan

Research output: Contribution to journalArticle

Abstract

Molecular diagnosis, chemo-diagnosis and physiological parameter have been applied for detecting the Ganoderma disease of coconut. Polyclonal antibodies (PAbs) raised against mycelial protein of Ganoderma, specific mycelial protein (62 kDa) of Ganoderma isolates and basidiocarp protein of Ganoderma were used for detection. All the PAbs could detect Ganoderma in diseased coconut root tissues in early stage of the disease before symptom expression by indirect - ELISA at the antiserum dilution of 1:1000 for mycelial protein, 1:700 for specific protein and 1:3000 for basidiocarp protein. Low cross reactions were observed with saprophytic fungi occurring in coconut roots and also with other basidiomycetous fungi. For polymerase chain reaction tests, the primer was generated from the internal transcribed spacer region one (ITS 1) of rDNA of Ganoderma, which produced a PCR product of 167 bp in size. Utility of this method was confirmed at the field level.

Original languageEnglish
Pages (from-to)396-406
Number of pages11
JournalArchives of Phytopathology and Plant Protection
Volume41
Issue number6
DOIs
Publication statusPublished - Sep 2008

Fingerprint

Ganoderma
coconuts
enzyme-linked immunosorbent assay
basidiomata
proteins
polyclonal antibodies
methodology
fungi
cross reaction
internal transcribed spacers
antiserum
polymerase chain reaction

Keywords

  • Basidiomycetous
  • ELISA
  • Ganoderma
  • Internal transcribed spacer
  • Polyclonal antibodies
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Development and comparison of ELISA and PCR methods for the early detection of Ganoderma disease of coconut. / Karthikeyan, M.; Bhaskaran, R.; Radhika, K.; Mathiyazhagan, S.; Jayakumar, V.; Sandosskumar, R.; Velazhahan, R.

In: Archives of Phytopathology and Plant Protection, Vol. 41, No. 6, 09.2008, p. 396-406.

Research output: Contribution to journalArticle

Karthikeyan, M. ; Bhaskaran, R. ; Radhika, K. ; Mathiyazhagan, S. ; Jayakumar, V. ; Sandosskumar, R. ; Velazhahan, R. / Development and comparison of ELISA and PCR methods for the early detection of Ganoderma disease of coconut. In: Archives of Phytopathology and Plant Protection. 2008 ; Vol. 41, No. 6. pp. 396-406.
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