Determination of piroxicam in pharmaceutical formulations and urine samples using europium-sensitized luminescence

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Abstract

A simple, selective and sensitive luminescence method for the assay of piroxicam (PX) in aqueous solution is developed. The method is based on the luminescence sensitization of europium (Eu3+) by formation of ternary complex with PX in the presence of TOPO and Tween-80 as surfactant. The signal for Eu-PX-TOPO is monitored at λex=359 nm and λem=615 nm. Optimum conditions for the formation of the complex in sequential injection analysis (SIA) were 0.01 M Tris buffer, pH 7.5, TOPO 5.0×10-5 M, Tween-80 0.15% and 1.5 mM of Eu3+, which allows the determination of 100-1000 ppb of PX with limit of detection (LOD) of 29 ppb. The relative standard deviations of the method range between 0.5% and 3.9% indicating excellent reproducibility of the method. The proposed method was successfully applied for the assay of PX in pharmaceutical formulations and in urine samples. Average recoveries of 100.8±1.7% was obtained in tablet, whereas a recovery of 97.5±2.0% was obtained for the total PX (PX+hydoxy-PX) in urine sample.

Original languageEnglish
Pages (from-to)291-296
Number of pages6
JournalJournal of Luminescence
Volume127
Issue number2
DOIs
Publication statusPublished - Dec 2007

Fingerprint

Europium
Piroxicam
Drug Compounding
urine
europium
Luminescence
recovery
Urine
luminescence
formulations
tablets
Pharmaceutical Preparations
standard deviation
buffers
surfactants
injection
aqueous solutions
Polysorbates
Assays
Recovery

Keywords

  • Europium
  • Piroxicam
  • Sensitized luminescence
  • Sequential injection
  • Urine

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Atomic and Molecular Physics, and Optics

Cite this

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title = "Determination of piroxicam in pharmaceutical formulations and urine samples using europium-sensitized luminescence",
abstract = "A simple, selective and sensitive luminescence method for the assay of piroxicam (PX) in aqueous solution is developed. The method is based on the luminescence sensitization of europium (Eu3+) by formation of ternary complex with PX in the presence of TOPO and Tween-80 as surfactant. The signal for Eu-PX-TOPO is monitored at λex=359 nm and λem=615 nm. Optimum conditions for the formation of the complex in sequential injection analysis (SIA) were 0.01 M Tris buffer, pH 7.5, TOPO 5.0×10-5 M, Tween-80 0.15{\%} and 1.5 mM of Eu3+, which allows the determination of 100-1000 ppb of PX with limit of detection (LOD) of 29 ppb. The relative standard deviations of the method range between 0.5{\%} and 3.9{\%} indicating excellent reproducibility of the method. The proposed method was successfully applied for the assay of PX in pharmaceutical formulations and in urine samples. Average recoveries of 100.8±1.7{\%} was obtained in tablet, whereas a recovery of 97.5±2.0{\%} was obtained for the total PX (PX+hydoxy-PX) in urine sample.",
keywords = "Europium, Piroxicam, Sensitized luminescence, Sequential injection, Urine",
author = "Al-Kindy, {Salma M Z} and Suliman, {Fakhr Eldin O} and Al-Wishahi, {Aisha A.} and Al-Lawati, {Haidar A J} and Muhammed Aoudia",
year = "2007",
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language = "English",
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pages = "291--296",
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T1 - Determination of piroxicam in pharmaceutical formulations and urine samples using europium-sensitized luminescence

AU - Al-Kindy, Salma M Z

AU - Suliman, Fakhr Eldin O

AU - Al-Wishahi, Aisha A.

AU - Al-Lawati, Haidar A J

AU - Aoudia, Muhammed

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N2 - A simple, selective and sensitive luminescence method for the assay of piroxicam (PX) in aqueous solution is developed. The method is based on the luminescence sensitization of europium (Eu3+) by formation of ternary complex with PX in the presence of TOPO and Tween-80 as surfactant. The signal for Eu-PX-TOPO is monitored at λex=359 nm and λem=615 nm. Optimum conditions for the formation of the complex in sequential injection analysis (SIA) were 0.01 M Tris buffer, pH 7.5, TOPO 5.0×10-5 M, Tween-80 0.15% and 1.5 mM of Eu3+, which allows the determination of 100-1000 ppb of PX with limit of detection (LOD) of 29 ppb. The relative standard deviations of the method range between 0.5% and 3.9% indicating excellent reproducibility of the method. The proposed method was successfully applied for the assay of PX in pharmaceutical formulations and in urine samples. Average recoveries of 100.8±1.7% was obtained in tablet, whereas a recovery of 97.5±2.0% was obtained for the total PX (PX+hydoxy-PX) in urine sample.

AB - A simple, selective and sensitive luminescence method for the assay of piroxicam (PX) in aqueous solution is developed. The method is based on the luminescence sensitization of europium (Eu3+) by formation of ternary complex with PX in the presence of TOPO and Tween-80 as surfactant. The signal for Eu-PX-TOPO is monitored at λex=359 nm and λem=615 nm. Optimum conditions for the formation of the complex in sequential injection analysis (SIA) were 0.01 M Tris buffer, pH 7.5, TOPO 5.0×10-5 M, Tween-80 0.15% and 1.5 mM of Eu3+, which allows the determination of 100-1000 ppb of PX with limit of detection (LOD) of 29 ppb. The relative standard deviations of the method range between 0.5% and 3.9% indicating excellent reproducibility of the method. The proposed method was successfully applied for the assay of PX in pharmaceutical formulations and in urine samples. Average recoveries of 100.8±1.7% was obtained in tablet, whereas a recovery of 97.5±2.0% was obtained for the total PX (PX+hydoxy-PX) in urine sample.

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