Determination of meloxicam using europium sensitized luminescence in the presence of co-luminescence reagents

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Abstract

A sensitive time- resolved luminescence method for the determination of meloxicam (MX) in methanol and in aqueous solution is described. The method is based on the luminescence sensitization of europium (Eu 3+) by formation of ternary complex with MX in the presence of 1,10- phenanthroline as coligand, Tween-80 as surfactant and gadolinium ion as a co-luminescence reagent. The signal for Eu- MX-1, 10- phenanthroline is monitored at λ ex=360 nm and λ em=620 nm. Optimum conditions for the formation of the complex in aqueous system were 0.01 M TRIS buffer, pH 8.0, 1,10- phenanthroline (6.0×10 -6 M), Gd 3+ (7.0×10 -6 M), Tween-80 (0.28%) and 1.75 mM of Eu 3+ which allows the determination of 20.800 ppb of MX with limit of detection (LOD) of 7 ppb. The relative standard deviations of the method range between 0.1 and 1.1% indicating excellent reproducibility of the method. The proposed method was successfully applied for the assay of MX in pharmaceutical formulations, plasma and in urine samples. Average recoveries of 99.8-1.1%, 100.2-0.9% and 100.9-1.1% were obtained for MX in tablet, plasma and urine sample respectively.

Original languageEnglish
Pages (from-to)467-474
Number of pages8
JournalJournal of Fluorescence
Volume22
Issue number1
DOIs
Publication statusPublished - Jan 2012

Fingerprint

meloxicam
Europium
Luminescence
Polysorbates
sensitization
Urine
Plasmas
Drug Compounding
pharmaceutical
Gadolinium
Surface-Active Agents
Tablets
Methanol
Limit of Detection
Assays
Buffers
Ions

Keywords

  • Co-luminescence
  • Meloxicam
  • Plasma
  • Sensitized luminescence
  • Urine

ASJC Scopus subject areas

  • Spectroscopy
  • Biochemistry
  • Clinical Biochemistry

Cite this

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title = "Determination of meloxicam using europium sensitized luminescence in the presence of co-luminescence reagents",
abstract = "A sensitive time- resolved luminescence method for the determination of meloxicam (MX) in methanol and in aqueous solution is described. The method is based on the luminescence sensitization of europium (Eu 3+) by formation of ternary complex with MX in the presence of 1,10- phenanthroline as coligand, Tween-80 as surfactant and gadolinium ion as a co-luminescence reagent. The signal for Eu- MX-1, 10- phenanthroline is monitored at λ ex=360 nm and λ em=620 nm. Optimum conditions for the formation of the complex in aqueous system were 0.01 M TRIS buffer, pH 8.0, 1,10- phenanthroline (6.0×10 -6 M), Gd 3+ (7.0×10 -6 M), Tween-80 (0.28{\%}) and 1.75 mM of Eu 3+ which allows the determination of 20.800 ppb of MX with limit of detection (LOD) of 7 ppb. The relative standard deviations of the method range between 0.1 and 1.1{\%} indicating excellent reproducibility of the method. The proposed method was successfully applied for the assay of MX in pharmaceutical formulations, plasma and in urine samples. Average recoveries of 99.8-1.1{\%}, 100.2-0.9{\%} and 100.9-1.1{\%} were obtained for MX in tablet, plasma and urine sample respectively.",
keywords = "Co-luminescence, Meloxicam, Plasma, Sensitized luminescence, Urine",
author = "Al-Kindy, {Salma Muhamed Z} and Al-Habsy, {Salim K.} and Suliman, {Fakhr Eldin O} and Al-Lawati, {Haidar A J}",
year = "2012",
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doi = "10.1007/s10895-011-0980-6",
language = "English",
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T1 - Determination of meloxicam using europium sensitized luminescence in the presence of co-luminescence reagents

AU - Al-Kindy, Salma Muhamed Z

AU - Al-Habsy, Salim K.

AU - Suliman, Fakhr Eldin O

AU - Al-Lawati, Haidar A J

PY - 2012/1

Y1 - 2012/1

N2 - A sensitive time- resolved luminescence method for the determination of meloxicam (MX) in methanol and in aqueous solution is described. The method is based on the luminescence sensitization of europium (Eu 3+) by formation of ternary complex with MX in the presence of 1,10- phenanthroline as coligand, Tween-80 as surfactant and gadolinium ion as a co-luminescence reagent. The signal for Eu- MX-1, 10- phenanthroline is monitored at λ ex=360 nm and λ em=620 nm. Optimum conditions for the formation of the complex in aqueous system were 0.01 M TRIS buffer, pH 8.0, 1,10- phenanthroline (6.0×10 -6 M), Gd 3+ (7.0×10 -6 M), Tween-80 (0.28%) and 1.75 mM of Eu 3+ which allows the determination of 20.800 ppb of MX with limit of detection (LOD) of 7 ppb. The relative standard deviations of the method range between 0.1 and 1.1% indicating excellent reproducibility of the method. The proposed method was successfully applied for the assay of MX in pharmaceutical formulations, plasma and in urine samples. Average recoveries of 99.8-1.1%, 100.2-0.9% and 100.9-1.1% were obtained for MX in tablet, plasma and urine sample respectively.

AB - A sensitive time- resolved luminescence method for the determination of meloxicam (MX) in methanol and in aqueous solution is described. The method is based on the luminescence sensitization of europium (Eu 3+) by formation of ternary complex with MX in the presence of 1,10- phenanthroline as coligand, Tween-80 as surfactant and gadolinium ion as a co-luminescence reagent. The signal for Eu- MX-1, 10- phenanthroline is monitored at λ ex=360 nm and λ em=620 nm. Optimum conditions for the formation of the complex in aqueous system were 0.01 M TRIS buffer, pH 8.0, 1,10- phenanthroline (6.0×10 -6 M), Gd 3+ (7.0×10 -6 M), Tween-80 (0.28%) and 1.75 mM of Eu 3+ which allows the determination of 20.800 ppb of MX with limit of detection (LOD) of 7 ppb. The relative standard deviations of the method range between 0.1 and 1.1% indicating excellent reproducibility of the method. The proposed method was successfully applied for the assay of MX in pharmaceutical formulations, plasma and in urine samples. Average recoveries of 99.8-1.1%, 100.2-0.9% and 100.9-1.1% were obtained for MX in tablet, plasma and urine sample respectively.

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