Determination of amlodipine using terbium-sensitized luminescence in the presence of europium(III) as a co-luminescence reagent

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A sensitive time-resolved luminescence method for the determination of amlodipine (AM) in methanol and in aqueous solution is described. The method is based on the luminescence sensitization of terbium (Tb3+) by formation of a ternary complex with AM in the presence of tri-n-octylphosphine oxide (TOPO) as co-ligand, dodecylbenzenesulfate as surfactant and europium ion as a co-luminescence reagent. The signal for Tb-AM-TOPO is monitored at λex = 242 nm and λem = 550 nm. Optimum conditions for the formation of the complex in aqueous system were 0.015 M Tris (hydroxylmethyl) amino methane buffer, pH 9.0, TOPO (1.0 × 10-4 M), Eu3+ (2.0 × 10-7 M), dodecylbenzenesulfate (0.14%) and 6.0 × 10-5 M of Tb3+, which allows the determination of 10-50 ppb of AM with a limit of detection of 1.2 ppb. The relative standard deviations of the method range between 0.1 and 0.2% indicated excellent reproducibility of the method. The proposed method was successfully applied for the assay of AM in pharmaceutical formulations and in plasma samples. Average recoveries of 98.5 ± 0.2% and 95.2 ± 0.2% were obtained for AM in tablet and plasma samples respectively.

Original languageEnglish
Pages (from-to)657-662
Number of pages6
Issue number6
Publication statusPublished - Sep 1 2014



  • Amlodipine
  • Co-luminescence
  • Pharmaceutical products
  • Plasma
  • Sensitized luminescence

ASJC Scopus subject areas

  • Biophysics
  • Chemistry (miscellaneous)
  • Medicine(all)

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