TY - JOUR
T1 - Determination of aminoglutethimide enantiomers in pharmaceutical formulations by capillary electrophoresis using methylated-β-cyclodextrin as a chiral selector and computational calculation for their respective inclusion complexes
AU - Elbashir, Abdalla A.
AU - Suliman, Fakhr Eldin O.
AU - Saad, Bahruddin
AU - Aboul-Enein, Hassan Y.
N1 - Funding Information:
Financial support of the work by a Universiti Sains Malaysia Research University scheme is gratefully acknowledged.
PY - 2009/2/15
Y1 - 2009/2/15
N2 - A capillary electrophoretic method for the separation of the aminoglutethimide (AGT) enantiomers using methylated-β-cyclodextrin (M-β-CD) as chiral selector is described. Several parameters affecting the separation were studied, including the type and concentration of chiral selector, buffer pH, voltage and temperature. Good chiral separation of the racemic mixture was achieved in less than 9 min with resolution factor Rs = 2.1, using a fused-silica capillary and a background electrolyte (BGE) of tris-phosphate buffer solution (50 mmol L-1, pH 3.0) containing 30 mg mL-1 of M-β-CD. The separation was carried out in normal polarity mode at 25 °C, 16 kV and using hydrostatic injection. Acceptable validation criteria for selectivity, linearity, precision, and accuracy/recovery were included. The proposed method was successfully applied to the assay of AGT enantiomers in pharmaceutical formulations. The computational calculations for the inclusion complexes of the R- and S-AGT-M-β-CD rationalized the reasons for the different migration times between the AGT enantiomers.
AB - A capillary electrophoretic method for the separation of the aminoglutethimide (AGT) enantiomers using methylated-β-cyclodextrin (M-β-CD) as chiral selector is described. Several parameters affecting the separation were studied, including the type and concentration of chiral selector, buffer pH, voltage and temperature. Good chiral separation of the racemic mixture was achieved in less than 9 min with resolution factor Rs = 2.1, using a fused-silica capillary and a background electrolyte (BGE) of tris-phosphate buffer solution (50 mmol L-1, pH 3.0) containing 30 mg mL-1 of M-β-CD. The separation was carried out in normal polarity mode at 25 °C, 16 kV and using hydrostatic injection. Acceptable validation criteria for selectivity, linearity, precision, and accuracy/recovery were included. The proposed method was successfully applied to the assay of AGT enantiomers in pharmaceutical formulations. The computational calculations for the inclusion complexes of the R- and S-AGT-M-β-CD rationalized the reasons for the different migration times between the AGT enantiomers.
KW - Aminoglutethimide
KW - Capillary electrophoresis
KW - Chiral analysis
KW - Computational calculations
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U2 - 10.1016/j.talanta.2008.09.029
DO - 10.1016/j.talanta.2008.09.029
M3 - Article
C2 - 19084654
AN - SCOPUS:57349119858
SN - 0039-9140
VL - 77
SP - 1388
EP - 1393
JO - Talanta
JF - Talanta
IS - 4
ER -