Detection of equid herpesviruses 2 and 5 in a herd of 266 Lipizzaners in association with ocular findings

James Oliver Rushton, Jolanta Kolodziejek, Alexander Tichy, Barbara Nell, Norbert Nowotny

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A herd of Lipizzaners (n=266) in three locations [Federal stud Piber (Styria), Heldenberg (Lower Austria) and the Spanish Riding School Vienna (Vienna)] were examined for ocular diseases and tested for herpesviruses (HVs) in PBMCs, nasal- and conjunctival swabs. In 167 (62.8%) horses equid herpesvirus-2 (EHV-2) nucleic acid was identified in at least one of the investigated samples, and 136 (51.1%) horses were positive for equid herpesvirus-5 (EHV-5) nucleic acid in at least one of the samples. One hundred and five (39.5%) of the horses mentioned above were identified with EHV-2/EHV-5 double infections. The results were correlated with potential influence factors such as age, gender, location, and housing. Statistical analysis demonstrated a significantly higher percentage of horses diagnosed with HV infections stabled in one location (Piber); also, housing in free stalls was associated with a higher rate of HV infections. Horses carrying EHV-2 or EHV-5 nucleic acid were significantly younger than the negative group. Statistical analysis of PCR results and ophthalmic findings did not prove a significant association between any disease pattern and presence of the equid gammaherpesviruses EHV-2 and EHV-5. The results of this herd survey indicate that horses younger than eight years of age, housed in free stalls with high population densities have a significantly increased risk for infection with EHV-2 and EHV-5. However no prediction can be made whether this results in ophthalmic disease. This is the first ophthalmic-virologic survey of this magnitude in a single horse breed.

Original languageEnglish
Pages (from-to)139-144
Number of pages6
JournalVeterinary Microbiology
Volume164
Issue number1-2
DOIs
Publication statusPublished - May 31 2013

Fingerprint

Equid herpesvirus 5
Rhadinovirus
Equid herpesvirus 2
Herpesviridae
Horses
eyes
herds
horses
Austria
nucleic acids
eye diseases
free stalls
Nucleic Acids
Herpesviridae Infections
Eye Diseases
statistical analysis
horse breeds
infection
Population Density
Infection

Keywords

  • EHV-2
  • EHV-5
  • Equid herpesviruses
  • Horse
  • Ophthalmic findings
  • PCR
  • Sequencing

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Cite this

Detection of equid herpesviruses 2 and 5 in a herd of 266 Lipizzaners in association with ocular findings. / Rushton, James Oliver; Kolodziejek, Jolanta; Tichy, Alexander; Nell, Barbara; Nowotny, Norbert.

In: Veterinary Microbiology, Vol. 164, No. 1-2, 31.05.2013, p. 139-144.

Research output: Contribution to journalArticle

Rushton, James Oliver ; Kolodziejek, Jolanta ; Tichy, Alexander ; Nell, Barbara ; Nowotny, Norbert. / Detection of equid herpesviruses 2 and 5 in a herd of 266 Lipizzaners in association with ocular findings. In: Veterinary Microbiology. 2013 ; Vol. 164, No. 1-2. pp. 139-144.
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