Detection, genotyping, and antigen distribution of bovine viral diarrhoea virus in archival formalin fixed paraffin embedded tissues

H. Ali*, A. Ahmed, A. A. Ali, M. S. Atta, P. Hanna, A. Cepica

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

This report identifies and genotypes the bovine viral diarrhoea virus (BVDV) in up to 20 years old archival formalin fixed paraffin embedded (FFPE) tissues of bovine foetuses and calves. Samples from 6 presumably BVDV aborted foetuses and 3 calves diagnosed with mucosal disease were tested by immunohistochemistry (IHC), RT-PCR and a one-step multiplex real time RT-PCR for genotyping. Viral RNA was detected in a 20 years old sample from an aborted foetus at which multiple foci of BVDV viral antigen was localized by IHC in the pulmonary tissue. All three calves with mucosal disease were also RT-PCR positive and BVDV-1 was typed using the one-step multiplex real time RT-PCR. The BVDV antigen distribution was demonstrated in the cerebral cortex (in neurons and their processes and in some glial cells), lung, kidneys, oesophagus, tongue, buccal mucosa and ear pinna of one calve affected with mucosal disease. The highest intensity of the antigen signals were seen in the stratum basale and stratum spinosum of the mucosa of the upper digestive tract tissues. The purpose of this study is to further elucidate the diagnosis and genotyping of BVDV infection with respect to prospective and retrospective identification of the virus in routinely processed and stored tissues for histological evaluation.

Original languageEnglish
Pages (from-to)219-226
Number of pages8
JournalRevue de Medecine Veterinaire
Volume163
Issue number5
Publication statusPublished - 2012

Keywords

  • Abortion
  • Antigen distribution
  • Bovine viral diarrhoea virus
  • Formalin fixed paraffin embedded tissues
  • Genotyping
  • Mucosal disease

ASJC Scopus subject areas

  • veterinary(all)

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