@article{2ead1c3a67b1423d9ff2edaebc343648,
title = "Deletion of TRPV4 enhances in vitro wound healing of murine esophageal keratinocytes",
abstract = "Transient receptor potential vanilloid 4 (TRPV4) is a non-selective cation channel that is widely expressed in different body tissues and plays several physiological roles. This channel is highly expressed in esophageal keratinocytes where its activation mediates ATP release. However, whether TRPV4 has a role in wound healing of esophageal keratinocytes is unclear. In this study, we demonstrated that both cell migration and proliferation were slower in wild-type esophageal keratinocytes compared to cells having TRPV4 knockout. Our results suggest that TRPV4-mediated release of ATP from esophageal keratinocytes contributes to a decrease in the rate of in vitro wound healing via the ATP degradation product adenosine, which acts on A2B adenosine receptors.",
author = "Ammar Boudaka and Saito, {Claire T.} and Makoto Tominaga",
note = "Funding Information: The authors are grateful to Prof. M. Nishida (Division of Cardiocirculatory Signaling at the National Institute for Physiological Sciences) for his technical support and expertise in the ShellPa mechanical cell strain method. The authors are also grateful to Yu Yamanoi and Sandra Derouiche for their technical support in time-lapse and transfection experiments, respectively. This work was supported by grants to MT from a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology in Japan (#15H02501 and #15H05928; Scientific Research on Innovative Areas {\textquoteleft}Thermal Biology{\textquoteright}). Publisher Copyright: {\textcopyright} 2020, The Author(s).",
year = "2020",
month = dec,
day = "1",
doi = "10.1038/s41598-020-68269-8",
language = "English",
volume = "10",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",
}