Degradation of petroleum model compounds immobilized on clay by a hypersaline microbial mat

Stefan Grötzschel, Jürgen Köster, Raeid M M Abed, Dirk De Beer

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

In this study the degradation of hydrophobic petroleum model compounds (phenanthrene, pristane, octadecane and dibenzothiophene) added to a submersed hypersaline microbial mat was investigated. Montmorillonite with an artificially altered, hydrophobic surface was used as carrier material, forming an organo-clay complex (OCC) with the attached mixture of petroleum model compounds. 6 mg/cm2 OCC were applied to cyanobacterial mat pieces, containing ∼33.3 μg/mg OCC of each compound. The degradation experiment was performed under controlled laboratory conditions and accompanied by chemical analyses by GC/GC-MS, molecular analyses by PCR and DGGE as well as functional analyses by microsensor measurements of oxygen, photosynthesis, sulfide, pH and light. All applied model compounds were degraded, but residues were still present after 18 weeks. The aromatic compounds phenanthrene (5.1 μg/mg OCC) and dibenzothiophene (4.3 μg/mg OCC) were preferentially degraded compared to the alkanes pristane (12.4 μg/mg OCC) and n-octadecane (13.4 μg/mg OCC). Metabolic changes during the degradation process could not be detected by microsensor measurements. The molecular population analyses did not reveal any significant community changes concomitant with the decrease of the petroleum model compounds. We conclude, that the pristine mats represent an intact, robust ecosystem in which the enzymatic requirements for the degradation of the applied pollutants exist. The slow degradation process did not affect the usual high internal turnover rates and did not favor a certain population in the community of the mats.

Original languageEnglish
Pages (from-to)273-283
Number of pages11
JournalBiodegradation
Volume13
Issue number4
DOIs
Publication statusPublished - 2002

Fingerprint

organoclay
Organoclay
microbial mat
Petroleum
Clay
Crude oil
petroleum
clay
Degradation
degradation
Pristane
Microsensors
phenanthrene
Bentonite
Alkanes
Photosynthesis
Aromatic compounds
Sulfides
Clay minerals
montmorillonite

Keywords

  • Cyanobacteria
  • Dibenzothiophene
  • Microsensors
  • Octadecane
  • PCR-DGGE
  • Phenanthrene
  • Pristane

ASJC Scopus subject areas

  • Biotechnology

Cite this

Degradation of petroleum model compounds immobilized on clay by a hypersaline microbial mat. / Grötzschel, Stefan; Köster, Jürgen; Abed, Raeid M M; De Beer, Dirk.

In: Biodegradation, Vol. 13, No. 4, 2002, p. 273-283.

Research output: Contribution to journalArticle

Grötzschel, Stefan ; Köster, Jürgen ; Abed, Raeid M M ; De Beer, Dirk. / Degradation of petroleum model compounds immobilized on clay by a hypersaline microbial mat. In: Biodegradation. 2002 ; Vol. 13, No. 4. pp. 273-283.
@article{3711b1af64ec4db0bfc01846b6894d39,
title = "Degradation of petroleum model compounds immobilized on clay by a hypersaline microbial mat",
abstract = "In this study the degradation of hydrophobic petroleum model compounds (phenanthrene, pristane, octadecane and dibenzothiophene) added to a submersed hypersaline microbial mat was investigated. Montmorillonite with an artificially altered, hydrophobic surface was used as carrier material, forming an organo-clay complex (OCC) with the attached mixture of petroleum model compounds. 6 mg/cm2 OCC were applied to cyanobacterial mat pieces, containing ∼33.3 μg/mg OCC of each compound. The degradation experiment was performed under controlled laboratory conditions and accompanied by chemical analyses by GC/GC-MS, molecular analyses by PCR and DGGE as well as functional analyses by microsensor measurements of oxygen, photosynthesis, sulfide, pH and light. All applied model compounds were degraded, but residues were still present after 18 weeks. The aromatic compounds phenanthrene (5.1 μg/mg OCC) and dibenzothiophene (4.3 μg/mg OCC) were preferentially degraded compared to the alkanes pristane (12.4 μg/mg OCC) and n-octadecane (13.4 μg/mg OCC). Metabolic changes during the degradation process could not be detected by microsensor measurements. The molecular population analyses did not reveal any significant community changes concomitant with the decrease of the petroleum model compounds. We conclude, that the pristine mats represent an intact, robust ecosystem in which the enzymatic requirements for the degradation of the applied pollutants exist. The slow degradation process did not affect the usual high internal turnover rates and did not favor a certain population in the community of the mats.",
keywords = "Cyanobacteria, Dibenzothiophene, Microsensors, Octadecane, PCR-DGGE, Phenanthrene, Pristane",
author = "Stefan Gr{\"o}tzschel and J{\"u}rgen K{\"o}ster and Abed, {Raeid M M} and {De Beer}, Dirk",
year = "2002",
doi = "10.1023/A:1021263009377",
language = "English",
volume = "13",
pages = "273--283",
journal = "Biodegradation",
issn = "0923-9820",
publisher = "Springer Netherlands",
number = "4",

}

TY - JOUR

T1 - Degradation of petroleum model compounds immobilized on clay by a hypersaline microbial mat

AU - Grötzschel, Stefan

AU - Köster, Jürgen

AU - Abed, Raeid M M

AU - De Beer, Dirk

PY - 2002

Y1 - 2002

N2 - In this study the degradation of hydrophobic petroleum model compounds (phenanthrene, pristane, octadecane and dibenzothiophene) added to a submersed hypersaline microbial mat was investigated. Montmorillonite with an artificially altered, hydrophobic surface was used as carrier material, forming an organo-clay complex (OCC) with the attached mixture of petroleum model compounds. 6 mg/cm2 OCC were applied to cyanobacterial mat pieces, containing ∼33.3 μg/mg OCC of each compound. The degradation experiment was performed under controlled laboratory conditions and accompanied by chemical analyses by GC/GC-MS, molecular analyses by PCR and DGGE as well as functional analyses by microsensor measurements of oxygen, photosynthesis, sulfide, pH and light. All applied model compounds were degraded, but residues were still present after 18 weeks. The aromatic compounds phenanthrene (5.1 μg/mg OCC) and dibenzothiophene (4.3 μg/mg OCC) were preferentially degraded compared to the alkanes pristane (12.4 μg/mg OCC) and n-octadecane (13.4 μg/mg OCC). Metabolic changes during the degradation process could not be detected by microsensor measurements. The molecular population analyses did not reveal any significant community changes concomitant with the decrease of the petroleum model compounds. We conclude, that the pristine mats represent an intact, robust ecosystem in which the enzymatic requirements for the degradation of the applied pollutants exist. The slow degradation process did not affect the usual high internal turnover rates and did not favor a certain population in the community of the mats.

AB - In this study the degradation of hydrophobic petroleum model compounds (phenanthrene, pristane, octadecane and dibenzothiophene) added to a submersed hypersaline microbial mat was investigated. Montmorillonite with an artificially altered, hydrophobic surface was used as carrier material, forming an organo-clay complex (OCC) with the attached mixture of petroleum model compounds. 6 mg/cm2 OCC were applied to cyanobacterial mat pieces, containing ∼33.3 μg/mg OCC of each compound. The degradation experiment was performed under controlled laboratory conditions and accompanied by chemical analyses by GC/GC-MS, molecular analyses by PCR and DGGE as well as functional analyses by microsensor measurements of oxygen, photosynthesis, sulfide, pH and light. All applied model compounds were degraded, but residues were still present after 18 weeks. The aromatic compounds phenanthrene (5.1 μg/mg OCC) and dibenzothiophene (4.3 μg/mg OCC) were preferentially degraded compared to the alkanes pristane (12.4 μg/mg OCC) and n-octadecane (13.4 μg/mg OCC). Metabolic changes during the degradation process could not be detected by microsensor measurements. The molecular population analyses did not reveal any significant community changes concomitant with the decrease of the petroleum model compounds. We conclude, that the pristine mats represent an intact, robust ecosystem in which the enzymatic requirements for the degradation of the applied pollutants exist. The slow degradation process did not affect the usual high internal turnover rates and did not favor a certain population in the community of the mats.

KW - Cyanobacteria

KW - Dibenzothiophene

KW - Microsensors

KW - Octadecane

KW - PCR-DGGE

KW - Phenanthrene

KW - Pristane

UR - http://www.scopus.com/inward/record.url?scp=0346037162&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0346037162&partnerID=8YFLogxK

U2 - 10.1023/A:1021263009377

DO - 10.1023/A:1021263009377

M3 - Article

C2 - 12521291

AN - SCOPUS:0346037162

VL - 13

SP - 273

EP - 283

JO - Biodegradation

JF - Biodegradation

SN - 0923-9820

IS - 4

ER -