Cytogenetic significance of chromosome 17 aberrations and P53 gene mutations as prognostic markers in oral squamous cell carcinoma

Walid Zedan, Mohamed I. Mourad, Abd M A El-Aziz, Nagla M. Salamaa, Asem A. Shalaby

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: Cytogenetic analysis has detected an accumulation of genetic lesions in oral cancers. Numerical changes in chromosome 17 might be associated with an up-regulation of p53 gene, and could contribute to critical events in carcinogenesis. The aim of this study was to reveal possible correlations between the numerical aberrations of chromosome 17, deletion or amplification of the P53 gene and histological grading in patients with oral squamous cell carcinoma (OSCC). Methods: This study was performed retrospectively on anonymous forty paraffin embedded specimens diagnosed with a primary OSCC. Sections were prepared for p53 immunohistochemical staining and FISH technique evaluation. Results: All studied cases showed a positive nuclear staining with different indices for the p53 protein. Furthermore, statistical analysis showed a significant difference between all histological types of OSCC. In term of P53 immunoreactivity well differentiated OSCC showed the highest, whereas poorly differentiated showed weakest. Regarding chromosome 17 aberrations and p53 gene mutations, Spearman correlation test revealed a statistical significant positive correlation between chromosome 17 abnormalities and p53 gene mutations as well as with the immunohistochemical expression of p53 proteins. Moreover, the positive association between p53 gene mutations and the expression of p53 protein was statistically significant. Conclusion: In the light of the previous findings, we concluded that numerical aberrations of chromosome 17 and p53 gene mutations as well as expression of p53 protein have enormous influence on various cellular processes including differentiation and carcinogenesis. Such knowledge provides an easy and simplified approach to prognosis predilection for OSCC.

Original languageEnglish
Article number2
JournalDiagnostic Pathology
Volume10
Issue number1
DOIs
Publication statusPublished - Jan 22 2015

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Chromosomes, Human, Pair 17
p53 Genes
Cytogenetics
Chromosome Aberrations
Squamous Cell Carcinoma
Mutation
Carcinogenesis
Proteins
Staining and Labeling
Cytogenetic Analysis
Mouth Neoplasms
Fluorescence In Situ Hybridization
Paraffin
Up-Regulation
Gene Expression

Keywords

  • Chromosome 17
  • FISH technique
  • Oral squamous cell carcinoma
  • P53 gene
  • P53 immunoreactivity

ASJC Scopus subject areas

  • Histology
  • Pathology and Forensic Medicine

Cite this

Cytogenetic significance of chromosome 17 aberrations and P53 gene mutations as prognostic markers in oral squamous cell carcinoma. / Zedan, Walid; Mourad, Mohamed I.; El-Aziz, Abd M A; Salamaa, Nagla M.; Shalaby, Asem A.

In: Diagnostic Pathology, Vol. 10, No. 1, 2, 22.01.2015.

Research output: Contribution to journalArticle

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