TY - JOUR
T1 - CRISPR/Cas9
T2 - A practical approach in date palm genome editing
AU - Sattar, Muhammad N.
AU - Iqbal, Zafar
AU - Tahir, Muhammad N.
AU - Shahid, Muhammad S.
AU - Khurshid, Muhammad
AU - Al-Khateeb, Abdullatif A.
AU - Al-Khateeb, Suliman A.
N1 - Publisher Copyright:
© 2017 Sattar, Iqbal, Tahir, Shahid, Khurshid, Al-Khateeb and Al-Khateeb.
PY - 2017/8/23
Y1 - 2017/8/23
N2 - The genetic modifications through breeding of crop plants have long been used to improve the yield and quality. However, precise genome editing (GE) could be a very useful supplementary tool for improvement of crop plants by targeted genome modifications. Various GE techniques including ZFNs (zinc finger nucleases), TALENs (transcription activator-like effector nucleases), and most recently clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 (CRISPR-associated protein 9)- based approaches have been successfully employed for various crop plants including fruit trees. CRISPR/Cas9-based approaches hold great potential in GE due to their simplicity, competency, and versatility over other GE techniques. However, to the best of our knowledge no such genetic improvement has ever been developed in date palm—an important fruit crop in Oasis agriculture. The applications of CRISPR/Cas9 can be a challenging task in date palm GE due to its large and complex genome, high rate of heterozygosity and outcrossing, in vitro regeneration and screening of mutants, high frequency of single-nucleotide polymorphism in the genome and ultimately genetic instability. In this review, we addressed the potential application of CRISPR/Cas9-based approaches in date palm GE to improve the sustainable date palm production. The availability of the date palm whole genome sequence has made it feasible to use CRISPR/Cas9 GE approach for genetic improvement in this species. Moreover, the future prospects of GE application in date palm are also addressed in this review.
AB - The genetic modifications through breeding of crop plants have long been used to improve the yield and quality. However, precise genome editing (GE) could be a very useful supplementary tool for improvement of crop plants by targeted genome modifications. Various GE techniques including ZFNs (zinc finger nucleases), TALENs (transcription activator-like effector nucleases), and most recently clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 (CRISPR-associated protein 9)- based approaches have been successfully employed for various crop plants including fruit trees. CRISPR/Cas9-based approaches hold great potential in GE due to their simplicity, competency, and versatility over other GE techniques. However, to the best of our knowledge no such genetic improvement has ever been developed in date palm—an important fruit crop in Oasis agriculture. The applications of CRISPR/Cas9 can be a challenging task in date palm GE due to its large and complex genome, high rate of heterozygosity and outcrossing, in vitro regeneration and screening of mutants, high frequency of single-nucleotide polymorphism in the genome and ultimately genetic instability. In this review, we addressed the potential application of CRISPR/Cas9-based approaches in date palm GE to improve the sustainable date palm production. The availability of the date palm whole genome sequence has made it feasible to use CRISPR/Cas9 GE approach for genetic improvement in this species. Moreover, the future prospects of GE application in date palm are also addressed in this review.
KW - CRISPR/Cas9
KW - Date palm
KW - Genome editing
KW - Loss of and gain-of-functions
KW - Multiplexing
UR - http://www.scopus.com/inward/record.url?scp=85029160219&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85029160219&partnerID=8YFLogxK
U2 - 10.3389/fpls.2017.01469
DO - 10.3389/fpls.2017.01469
M3 - Review article
AN - SCOPUS:85029160219
SN - 1664-462X
VL - 8
JO - Frontiers in Plant Science
JF - Frontiers in Plant Science
M1 - 1469
ER -