Covalent Immobilization of EPCs-Affinity Peptide on Poly(L-Lactide-co-ε-Caprolactone) Copolymers to Enhance EPCs Adhesion and Retention for Tissue Engineering Applications

Jongyoon Rhee, Muhammad Shafiq, Donghak Kim, Youngmee Jung, Soo Hyun Kim

Research output: Contribution to journalArticle

Abstract

Small diameter vascular grafts (inner diameter ≤ 6 mm) have a critical limitation regarding inner thrombotic reaction and occlude when implanted as artificial substitutes. In situ capture of endothelial progenitor cells (EPCs) could be beneficial to improve the endothelialization of artificial blood vessels. This study aimed to develop EPCs-affinity peptide (TPSLEQRTVYAK, TPS) and heparin-conjugated star-shaped poly(L-lactide-co-ε-caprolactone) (St-PLCL) copolymers to simultaneously capture EPCs and improve the hemocompatibility of vascular grafts, respectively. Electrospun membranes and small-diameter vascular grafts were fabricated by mixing linear PLCL, heparin-conjugated St-PLCL (PLCL-Hep), and TPS-conjugated St-PLCL (PLCL-TPS) copolymers. Vascular grafts exhibited biomechanical properties similar to the ISO standard. Membranes containing PLCL-Hep and PLCL-TPS showed fewer adhered platelets than did the control membranes. Moreover, electrospun membranes containing PLCL-Hep and PLCL-TPS adhered significantly to more EPCs than did the control group; however, three types of membranes did not appreciably differ in terms of the attachment of endothelial cells (ECs). Subcutaneous implantation of vascular grafts in Sprague-Dawley rats led to cellular infiltration and neotissue formation, which increased with the passage of time. Taken together, PLCL-TPS and PLCL-Hep copolymers can be fabricated into small-diameter vascular grafts to facilitate endothelialization through endogenous cell recruitment for vascular tissue regeneration applications. [Figure not available: see fulltext.]

Original languageEnglish
JournalMacromolecular Research
DOIs
Publication statusAccepted/In press - Jan 1 2018

Fingerprint

Cell adhesion
Endothelial cells
Tissue engineering
Grafts
Peptides
Copolymers
Heparin
Membranes
Stars
Blood vessel prostheses
Tissue regeneration
Platelets
Infiltration
poly(lactide)
caprolactone
Rats

Keywords

  • cell adhesion
  • endothelial progenitor cells
  • endothelialization
  • poly(L-lactide-co-ε-caprolactone)
  • polyester
  • TPS
  • vascular grafts

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

Cite this

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title = "Covalent Immobilization of EPCs-Affinity Peptide on Poly(L-Lactide-co-ε-Caprolactone) Copolymers to Enhance EPCs Adhesion and Retention for Tissue Engineering Applications",
abstract = "Small diameter vascular grafts (inner diameter ≤ 6 mm) have a critical limitation regarding inner thrombotic reaction and occlude when implanted as artificial substitutes. In situ capture of endothelial progenitor cells (EPCs) could be beneficial to improve the endothelialization of artificial blood vessels. This study aimed to develop EPCs-affinity peptide (TPSLEQRTVYAK, TPS) and heparin-conjugated star-shaped poly(L-lactide-co-ε-caprolactone) (St-PLCL) copolymers to simultaneously capture EPCs and improve the hemocompatibility of vascular grafts, respectively. Electrospun membranes and small-diameter vascular grafts were fabricated by mixing linear PLCL, heparin-conjugated St-PLCL (PLCL-Hep), and TPS-conjugated St-PLCL (PLCL-TPS) copolymers. Vascular grafts exhibited biomechanical properties similar to the ISO standard. Membranes containing PLCL-Hep and PLCL-TPS showed fewer adhered platelets than did the control membranes. Moreover, electrospun membranes containing PLCL-Hep and PLCL-TPS adhered significantly to more EPCs than did the control group; however, three types of membranes did not appreciably differ in terms of the attachment of endothelial cells (ECs). Subcutaneous implantation of vascular grafts in Sprague-Dawley rats led to cellular infiltration and neotissue formation, which increased with the passage of time. Taken together, PLCL-TPS and PLCL-Hep copolymers can be fabricated into small-diameter vascular grafts to facilitate endothelialization through endogenous cell recruitment for vascular tissue regeneration applications. [Figure not available: see fulltext.]",
keywords = "cell adhesion, endothelial progenitor cells, endothelialization, poly(L-lactide-co-ε-caprolactone), polyester, TPS, vascular grafts",
author = "Jongyoon Rhee and Muhammad Shafiq and Donghak Kim and Youngmee Jung and Kim, {Soo Hyun}",
year = "2018",
month = "1",
day = "1",
doi = "10.1007/s13233-019-7003-x",
language = "English",
journal = "Macromolecular Research",
issn = "1598-5032",
publisher = "Polymer Society of Korea",

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T1 - Covalent Immobilization of EPCs-Affinity Peptide on Poly(L-Lactide-co-ε-Caprolactone) Copolymers to Enhance EPCs Adhesion and Retention for Tissue Engineering Applications

AU - Rhee, Jongyoon

AU - Shafiq, Muhammad

AU - Kim, Donghak

AU - Jung, Youngmee

AU - Kim, Soo Hyun

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Small diameter vascular grafts (inner diameter ≤ 6 mm) have a critical limitation regarding inner thrombotic reaction and occlude when implanted as artificial substitutes. In situ capture of endothelial progenitor cells (EPCs) could be beneficial to improve the endothelialization of artificial blood vessels. This study aimed to develop EPCs-affinity peptide (TPSLEQRTVYAK, TPS) and heparin-conjugated star-shaped poly(L-lactide-co-ε-caprolactone) (St-PLCL) copolymers to simultaneously capture EPCs and improve the hemocompatibility of vascular grafts, respectively. Electrospun membranes and small-diameter vascular grafts were fabricated by mixing linear PLCL, heparin-conjugated St-PLCL (PLCL-Hep), and TPS-conjugated St-PLCL (PLCL-TPS) copolymers. Vascular grafts exhibited biomechanical properties similar to the ISO standard. Membranes containing PLCL-Hep and PLCL-TPS showed fewer adhered platelets than did the control membranes. Moreover, electrospun membranes containing PLCL-Hep and PLCL-TPS adhered significantly to more EPCs than did the control group; however, three types of membranes did not appreciably differ in terms of the attachment of endothelial cells (ECs). Subcutaneous implantation of vascular grafts in Sprague-Dawley rats led to cellular infiltration and neotissue formation, which increased with the passage of time. Taken together, PLCL-TPS and PLCL-Hep copolymers can be fabricated into small-diameter vascular grafts to facilitate endothelialization through endogenous cell recruitment for vascular tissue regeneration applications. [Figure not available: see fulltext.]

AB - Small diameter vascular grafts (inner diameter ≤ 6 mm) have a critical limitation regarding inner thrombotic reaction and occlude when implanted as artificial substitutes. In situ capture of endothelial progenitor cells (EPCs) could be beneficial to improve the endothelialization of artificial blood vessels. This study aimed to develop EPCs-affinity peptide (TPSLEQRTVYAK, TPS) and heparin-conjugated star-shaped poly(L-lactide-co-ε-caprolactone) (St-PLCL) copolymers to simultaneously capture EPCs and improve the hemocompatibility of vascular grafts, respectively. Electrospun membranes and small-diameter vascular grafts were fabricated by mixing linear PLCL, heparin-conjugated St-PLCL (PLCL-Hep), and TPS-conjugated St-PLCL (PLCL-TPS) copolymers. Vascular grafts exhibited biomechanical properties similar to the ISO standard. Membranes containing PLCL-Hep and PLCL-TPS showed fewer adhered platelets than did the control membranes. Moreover, electrospun membranes containing PLCL-Hep and PLCL-TPS adhered significantly to more EPCs than did the control group; however, three types of membranes did not appreciably differ in terms of the attachment of endothelial cells (ECs). Subcutaneous implantation of vascular grafts in Sprague-Dawley rats led to cellular infiltration and neotissue formation, which increased with the passage of time. Taken together, PLCL-TPS and PLCL-Hep copolymers can be fabricated into small-diameter vascular grafts to facilitate endothelialization through endogenous cell recruitment for vascular tissue regeneration applications. [Figure not available: see fulltext.]

KW - cell adhesion

KW - endothelial progenitor cells

KW - endothelialization

KW - poly(L-lactide-co-ε-caprolactone)

KW - polyester

KW - TPS

KW - vascular grafts

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AN - SCOPUS:85053390965

JO - Macromolecular Research

JF - Macromolecular Research

SN - 1598-5032

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