Cotton leaf curl Rajasthan virus infecting tomato in Pakistan

Muhammad Shafiq Shahid, Liaqat Ali, Saiqa Wajid

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Tomato plants showing phenotypically symptoms of tomato leaf curl disease (ToLCD) were collected in Faisalabad, Pakistan. These exhibited a severe downward leaf curling with enation on the lower side of the leaf. In order to identify the begomovirus components associated with the disease phenotypes, DNA extracted from them was screened by PCR using specific primers for ToLCNDV DNA A and DNA B and a universal beta satellite primer pair (designed to detect all beta satellite). Tomato sample was found positive for ToLCNDV DNAA and beta satellite. The fragments amplified were cloned and sequenced. The begomovirus sequence obtained showed the highest levels of sequence identity (99%) to cotton leaf curl Rajasthan virus (CLCuRV), a virus previously identified in cotton showing symptoms of cotton leaf curl disease (CLCuD). The sequence of beta satellite showed 99% identity to the beta satellite associated with CLCuD. This is the first time CLCuRV has been identified in tomato and indicates that this host can serve as a reservoir for the agent causing CLCuD. Partial repeat constructs for Agrobacterium-mediated inoculation have been produced to show infectivity of these clones (to fulfill Koch's postulates), for studying their host range and potential threat to crops.

Original languageEnglish
Pages (from-to)319-321
Number of pages3
JournalPakistan Journal of Scientific and Industrial Research
Volume52
Issue number6
Publication statusPublished - Nov 2009

Fingerprint

leaf curling
Pakistan
tomatoes
cotton
Begomovirus
signs and symptoms (plants)
DNA primers
DNA
Agrobacterium
host range
pathogenicity
Cotton leaf curl Multan virus
clones
phenotype
viruses
crops
leaves
sampling

Keywords

  • Begomovirus
  • Beta satellite
  • CLCuD
  • CLCuRV in tomato
  • Geminivirus
  • Tomato disease
  • Whitefly

ASJC Scopus subject areas

  • General

Cite this

Cotton leaf curl Rajasthan virus infecting tomato in Pakistan. / Shahid, Muhammad Shafiq; Ali, Liaqat; Wajid, Saiqa.

In: Pakistan Journal of Scientific and Industrial Research, Vol. 52, No. 6, 11.2009, p. 319-321.

Research output: Contribution to journalArticle

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