Coordinated release of acylation stimulating protein (ASP) and triacylglycerol clearance by human adipose tissue in vivo in the postprandial period

Jumana Saleh, Lucinda K M Summers, Katherine Cianflone, Barbara A. Fielding, Allan D. Sniderman, Keith N. Frayn

Research output: Contribution to journalArticle

131 Citations (Scopus)

Abstract

The objective of this study was to determine whether Acylation Stimulating Protein (ASP) is generated in vivo by human adipose tissue during the postprandial period. After a fat meal, samples from 12 subjects were obtained (up to 6 h) from an arterialized hand vein and an anterior abdominal wall vein that drains adipose tissue. Veno-arterial (V-A) gradients across the subcutaneous adipose tissue bed were calculated. The data demonstrate that ASP is produced in vivo (positive V-A gradient) with maximal production at 3-5 h postprandially. The plasma triacylglycerol (TAG) clearance was evidenced by a negative V-A gradient. It increased substantially after 3 h and remained prominant until the final time point. There was, therefore, a close temporal coordination between ASP generation and TAG clearance. In contrast, plasma insulin and non-esterified fatty acid (NEFA) had an early (1-2 h) postprandial change. Fatty acid incorporation into adipose tissue (FIAT) was calculated from V-A glycerol and non-esterified fatty acid (NEFA) differences postprandially. FIAT was negative during the first hour, implying net fat mobilization. FIAT then became increasingly positive, implying net fat deposition, and overall followed the same time course as ASP and TAG clearance. There was a direct positive correlation between total ASP production and total FIAT (r = 0.566, P <0.05). These data demonstrate that ASP is generated in vivo by human adipocytes and that this process is accentuated postprandially, supporting the concept that ASP plays an important role in clearance of TAG from plasma and fatty acid storage in adipose tissue.

Original languageEnglish
Pages (from-to)884-891
Number of pages8
JournalJournal of Lipid Research
Volume39
Issue number4
Publication statusPublished - Apr 1998

Fingerprint

Postprandial Period
Adipose Tissue
Triglycerides
Fatty Acids
Tissue
Fats
Plasmas
Veins
Subcutaneous Fat
Abdominal Wall
des-Arg-(77)-complement C3a
Adipocytes
Glycerol
Meals
Hand
Insulin

Keywords

  • Adipose tissue
  • Complement 3A
  • Human
  • Postprandial lipemia

ASJC Scopus subject areas

  • Endocrinology

Cite this

Coordinated release of acylation stimulating protein (ASP) and triacylglycerol clearance by human adipose tissue in vivo in the postprandial period. / Saleh, Jumana; Summers, Lucinda K M; Cianflone, Katherine; Fielding, Barbara A.; Sniderman, Allan D.; Frayn, Keith N.

In: Journal of Lipid Research, Vol. 39, No. 4, 04.1998, p. 884-891.

Research output: Contribution to journalArticle

Saleh, Jumana ; Summers, Lucinda K M ; Cianflone, Katherine ; Fielding, Barbara A. ; Sniderman, Allan D. ; Frayn, Keith N. / Coordinated release of acylation stimulating protein (ASP) and triacylglycerol clearance by human adipose tissue in vivo in the postprandial period. In: Journal of Lipid Research. 1998 ; Vol. 39, No. 4. pp. 884-891.
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