Concentrations of Parabens in human breast tumours

P. D. Darbre, A. Aljarrah, W. R. Miller, N. G. Coldham, M. J. Sauer, G. S. Pope

Research output: Contribution to journalArticle

468 Citations (Scopus)

Abstract

Parabens are used as preservatives in many thousands of cosmetic, food and pharmaceutical products to which the human population is exposed. Although recent reports of the oestrogenic properties of parabens have challenged current concepts of their toxicity in these consumer products, the question remains as to whether any of the parabens can accumulate intact in the body from the long-term, low-dose levels to which humans are exposed. Initial studies reported here show that parabens can be extracted from human breast tissue and detected by thin-layer chromatography. More detailed studies enabled identification and measurement of mean concentrations of individual parabens in samples of 20 human breast tumours by high-pressure liquid chromatography followed by tandem mass spectrometry. The mean concentration of parabens in these 20 human breast tumours was found to be 20.6 ± 4.2 ng g-1 tissue. Comparison of individual parabens showed that methylparaben was present at the highest level (with a mean value of 12.8 ± 2.2 ng g-1 tissue) and represents 62% of the total paraben recovered in the extractions. These studies demonstrate that parabens can be found intact in the human breast and this should open the way technically for more detailed information to be obtained on body burdens of parabens and in particular whether body burdens are different in cancer from those in normal tissues.

Original languageEnglish
Pages (from-to)5-13
Number of pages9
JournalJournal of Applied Toxicology
Volume24
Issue number1
DOIs
Publication statusPublished - Jan 2004

Fingerprint

Parabens
Tumors
Breast Neoplasms
Tissue
Body Burden
Breast
Food preservatives
High pressure liquid chromatography
Thin layer chromatography
Consumer products
Cosmetics
Thin Layer Chromatography
Tandem Mass Spectrometry
Mass spectrometry
Toxicity
High Pressure Liquid Chromatography

Keywords

  • Cosmetics
  • HPLC-MS-MS
  • Human breast cancer
  • Oestrogenic activity
  • Parabens
  • Preservatives
  • Xenoestrogens

ASJC Scopus subject areas

  • Toxicology

Cite this

Darbre, P. D., Aljarrah, A., Miller, W. R., Coldham, N. G., Sauer, M. J., & Pope, G. S. (2004). Concentrations of Parabens in human breast tumours. Journal of Applied Toxicology, 24(1), 5-13. https://doi.org/10.1002/jat.958

Concentrations of Parabens in human breast tumours. / Darbre, P. D.; Aljarrah, A.; Miller, W. R.; Coldham, N. G.; Sauer, M. J.; Pope, G. S.

In: Journal of Applied Toxicology, Vol. 24, No. 1, 01.2004, p. 5-13.

Research output: Contribution to journalArticle

Darbre, PD, Aljarrah, A, Miller, WR, Coldham, NG, Sauer, MJ & Pope, GS 2004, 'Concentrations of Parabens in human breast tumours', Journal of Applied Toxicology, vol. 24, no. 1, pp. 5-13. https://doi.org/10.1002/jat.958
Darbre, P. D. ; Aljarrah, A. ; Miller, W. R. ; Coldham, N. G. ; Sauer, M. J. ; Pope, G. S. / Concentrations of Parabens in human breast tumours. In: Journal of Applied Toxicology. 2004 ; Vol. 24, No. 1. pp. 5-13.
@article{20c0971ba8b04a9b9fcafcb430f72b70,
title = "Concentrations of Parabens in human breast tumours",
abstract = "Parabens are used as preservatives in many thousands of cosmetic, food and pharmaceutical products to which the human population is exposed. Although recent reports of the oestrogenic properties of parabens have challenged current concepts of their toxicity in these consumer products, the question remains as to whether any of the parabens can accumulate intact in the body from the long-term, low-dose levels to which humans are exposed. Initial studies reported here show that parabens can be extracted from human breast tissue and detected by thin-layer chromatography. More detailed studies enabled identification and measurement of mean concentrations of individual parabens in samples of 20 human breast tumours by high-pressure liquid chromatography followed by tandem mass spectrometry. The mean concentration of parabens in these 20 human breast tumours was found to be 20.6 ± 4.2 ng g-1 tissue. Comparison of individual parabens showed that methylparaben was present at the highest level (with a mean value of 12.8 ± 2.2 ng g-1 tissue) and represents 62{\%} of the total paraben recovered in the extractions. These studies demonstrate that parabens can be found intact in the human breast and this should open the way technically for more detailed information to be obtained on body burdens of parabens and in particular whether body burdens are different in cancer from those in normal tissues.",
keywords = "Cosmetics, HPLC-MS-MS, Human breast cancer, Oestrogenic activity, Parabens, Preservatives, Xenoestrogens",
author = "Darbre, {P. D.} and A. Aljarrah and Miller, {W. R.} and Coldham, {N. G.} and Sauer, {M. J.} and Pope, {G. S.}",
year = "2004",
month = "1",
doi = "10.1002/jat.958",
language = "English",
volume = "24",
pages = "5--13",
journal = "Journal of Applied Toxicology",
issn = "0260-437X",
publisher = "John Wiley and Sons Ltd",
number = "1",

}

TY - JOUR

T1 - Concentrations of Parabens in human breast tumours

AU - Darbre, P. D.

AU - Aljarrah, A.

AU - Miller, W. R.

AU - Coldham, N. G.

AU - Sauer, M. J.

AU - Pope, G. S.

PY - 2004/1

Y1 - 2004/1

N2 - Parabens are used as preservatives in many thousands of cosmetic, food and pharmaceutical products to which the human population is exposed. Although recent reports of the oestrogenic properties of parabens have challenged current concepts of their toxicity in these consumer products, the question remains as to whether any of the parabens can accumulate intact in the body from the long-term, low-dose levels to which humans are exposed. Initial studies reported here show that parabens can be extracted from human breast tissue and detected by thin-layer chromatography. More detailed studies enabled identification and measurement of mean concentrations of individual parabens in samples of 20 human breast tumours by high-pressure liquid chromatography followed by tandem mass spectrometry. The mean concentration of parabens in these 20 human breast tumours was found to be 20.6 ± 4.2 ng g-1 tissue. Comparison of individual parabens showed that methylparaben was present at the highest level (with a mean value of 12.8 ± 2.2 ng g-1 tissue) and represents 62% of the total paraben recovered in the extractions. These studies demonstrate that parabens can be found intact in the human breast and this should open the way technically for more detailed information to be obtained on body burdens of parabens and in particular whether body burdens are different in cancer from those in normal tissues.

AB - Parabens are used as preservatives in many thousands of cosmetic, food and pharmaceutical products to which the human population is exposed. Although recent reports of the oestrogenic properties of parabens have challenged current concepts of their toxicity in these consumer products, the question remains as to whether any of the parabens can accumulate intact in the body from the long-term, low-dose levels to which humans are exposed. Initial studies reported here show that parabens can be extracted from human breast tissue and detected by thin-layer chromatography. More detailed studies enabled identification and measurement of mean concentrations of individual parabens in samples of 20 human breast tumours by high-pressure liquid chromatography followed by tandem mass spectrometry. The mean concentration of parabens in these 20 human breast tumours was found to be 20.6 ± 4.2 ng g-1 tissue. Comparison of individual parabens showed that methylparaben was present at the highest level (with a mean value of 12.8 ± 2.2 ng g-1 tissue) and represents 62% of the total paraben recovered in the extractions. These studies demonstrate that parabens can be found intact in the human breast and this should open the way technically for more detailed information to be obtained on body burdens of parabens and in particular whether body burdens are different in cancer from those in normal tissues.

KW - Cosmetics

KW - HPLC-MS-MS

KW - Human breast cancer

KW - Oestrogenic activity

KW - Parabens

KW - Preservatives

KW - Xenoestrogens

UR - http://www.scopus.com/inward/record.url?scp=0842322823&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0842322823&partnerID=8YFLogxK

U2 - 10.1002/jat.958

DO - 10.1002/jat.958

M3 - Article

VL - 24

SP - 5

EP - 13

JO - Journal of Applied Toxicology

JF - Journal of Applied Toxicology

SN - 0260-437X

IS - 1

ER -