Composition of mycoflora and aflatoxins in lupine seeds from the Sudan

Seifeldin Ahmed Faragalla El-Nagerabi, Abdulqader Elzein Elshafie

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Thirteen seed samples of lupines (Lupinus termis Forrsk.) were evaluated for their phytosanitary status by incubation on potato-dextrose agar (PDA) and moist sterile filter papers (blotter method) at 28±2°C. The seeds were also assayed for the presence of aflatoxins and toxigenic fungi. Forty-seven species and 10 varieties in 14 genera of fungi were recovered. Among these, 45 species and 10 varieties were new to lupine seeds, and 5 species and 2 varieties were new to the mycoflora of the Sudan. The genus Aspergillus (10 species, 8 varieties) was the most common, followed by Rhizopus (1 species), Fusarium (6 species) and Alternaria (5 species), while the remaining genera (Chaetomium, Cladosporium, Curvularia, Drechslera, Penicillium, Phoma, Emericella, Mucor, Sclerotium, Ulocladium) displayed lower levels of contamination. Of possible pathogens on lupine plants, Alternaria (5 species) (stem lesion) and F. oxysporum (root rot) were recovered from the seeds. Thin-layer chromatographic analysis of chloroform extracts of the 13 seed samples revealed that two samples contained very low concentrations of aflatoxins B 1 and B 2 (4.5-6.5 μg/kg).

Original languageEnglish
Pages (from-to)257-262
Number of pages6
JournalPhytopathologia Mediterranea
Volume39
Issue number2
Publication statusPublished - 2000

Fingerprint

Lupinus albus var. albus
Sudan
aflatoxins
seeds
Alternaria
Emericella
Ulocladium
Chaetomium
Sclerotium
Drechslera
toxigenic strains
Curvularia
Mucor
lesions (plant)
Phoma
Rhizopus
Cladosporium
root rot
Penicillium
thin layer chromatography

Keywords

  • Aflatoxins
  • Lupine
  • Lupinus termis
  • Mycoflora
  • Pathogens

ASJC Scopus subject areas

  • Plant Science
  • Horticulture
  • Agronomy and Crop Science

Cite this

Composition of mycoflora and aflatoxins in lupine seeds from the Sudan. / El-Nagerabi, Seifeldin Ahmed Faragalla; Elshafie, Abdulqader Elzein.

In: Phytopathologia Mediterranea, Vol. 39, No. 2, 2000, p. 257-262.

Research output: Contribution to journalArticle

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