Complete nucleotide sequence of a monopartite Begomovirus and associated satellites infecting Carica papaya in Nepal

M. S. Shahid, S. Yoshida, G. B. Khatri-Chhetri, R. W. Briddon, K. T. Natsuaki

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Carica papaya (papaya) is a fruit crop that is cultivated mostly in kitchen gardens throughout Nepal. Leaf samples of C. papaya plants with leaf curling, vein darkening, vein thickening, and a reduction in leaf size were collected from a garden in Darai village, Rampur, Nepal in 2010. Full-length clones of a monopartite Begomovirus, a betasatellite and an alphasatellite were isolated. The complete nucleotide sequence of the Begomovirus showed the arrangement of genes typical of Old World begomoviruses with the highest nucleotide sequence identity (>99 %) to an isolate of Ageratum yellow vein virus (AYVV), confirming it as an isolate of AYVV. The complete nucleotide sequence of betasatellite showed greater than 89 % nucleotide sequence identity to an isolate of Tomato leaf curl Java betasatellite originating from Indonesian. The sequence of the alphasatellite displayed 92 % nucleotide sequence identity to Sida yellow vein China alphasatellite. This is the first identification of these components in Nepal and the first time they have been identified in papaya.

Original languageEnglish
Pages (from-to)581-584
Number of pages4
JournalVirus Genes
Volume46
Issue number3
DOIs
Publication statusPublished - Jun 2013

Fingerprint

Begomovirus
Carica
Nepal
Veins
Ageratum
Viruses
Gene Order
Plant Leaves
Lycopersicon esculentum
Fruit
China
Clone Cells

Keywords

  • Alphasatellite
  • Begomovirus
  • Betasatellite
  • Geminivirus
  • Papaya

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Virology

Cite this

Complete nucleotide sequence of a monopartite Begomovirus and associated satellites infecting Carica papaya in Nepal. / Shahid, M. S.; Yoshida, S.; Khatri-Chhetri, G. B.; Briddon, R. W.; Natsuaki, K. T.

In: Virus Genes, Vol. 46, No. 3, 06.2013, p. 581-584.

Research output: Contribution to journalArticle

Shahid, M. S. ; Yoshida, S. ; Khatri-Chhetri, G. B. ; Briddon, R. W. ; Natsuaki, K. T. / Complete nucleotide sequence of a monopartite Begomovirus and associated satellites infecting Carica papaya in Nepal. In: Virus Genes. 2013 ; Vol. 46, No. 3. pp. 581-584.
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