Comparison of the neuropathology induced by two West Nile virus strains

Emilie Donadieu, Steeve Lowenski, Jean Luc Servely, Eve Laloy, Thomas Lilin, Norbert Nowotny, Jennifer Richardson, Stéphan Zientara, Sylvie Lecollinet, Muriel Coulpier

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Some strains of West Nile virus (WNV) are neuroinvasive and may induce fatal encephalitis/meningitis in a variety of animal species including humans. Whether, however, there is a strain-specific signature in the brain is as yet unknown. Here we investigated the neuropathogenesis induced by two phylogenetically distant WNV strains of lineage 1, WNVIS98 and WNVKUN35 911. While four-week old C57Bl/6J mice were susceptible to both strains and succumbed rapidly after intraperitoneal inoculation, differences were observed in virulence and clinical disease. WNV KUN35 911, the less virulent strain as judged by determination of LD50, induced typical signs of encephalitis. Such signs were not observed in WNVIS98-infected mice, although they died more rapidly. Histological examination of brain sections also revealed differences, as the level of apoptosis and inflammation was higher in WNVKUN35 911- than WNVIS98-infected mice. Moreover, staining for cleaved caspase 3 showed that the two WNV strains induced apoptotic death through different molecular mechanisms in one particular brain area. Finally, the two strains showed similar tropism in cortex, striatum, brainstem, and cerebellum but a different one in hippocampus. In summary, our data show that, upon peripheral administration, WNVIS98 and WNVKUN35 911 strains induce partially distinct lesions and tissue tropism in the brain. They suggest that the virulence of a WNV strain is not necessarily correlated with the severity of apoptotic and inflammatory lesions in the brain.

Original languageEnglish
Article numbere84473
JournalPLoS One
Volume8
Issue number12
DOIs
Publication statusPublished - Dec 18 2013

Fingerprint

neuropathology
West Nile virus
Viruses
Brain
Tropism
Encephalitis
brain
Virulence
encephalitis
lesions (animal)
Lethal Dose 50
mice
virulence
Meningitis
Caspase 3
Cerebellum
Brain Stem
tissue tropism
Hippocampus
Animals

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Donadieu, E., Lowenski, S., Servely, J. L., Laloy, E., Lilin, T., Nowotny, N., ... Coulpier, M. (2013). Comparison of the neuropathology induced by two West Nile virus strains. PLoS One, 8(12), [e84473]. https://doi.org/10.1371/journal.pone.0084473

Comparison of the neuropathology induced by two West Nile virus strains. / Donadieu, Emilie; Lowenski, Steeve; Servely, Jean Luc; Laloy, Eve; Lilin, Thomas; Nowotny, Norbert; Richardson, Jennifer; Zientara, Stéphan; Lecollinet, Sylvie; Coulpier, Muriel.

In: PLoS One, Vol. 8, No. 12, e84473, 18.12.2013.

Research output: Contribution to journalArticle

Donadieu, E, Lowenski, S, Servely, JL, Laloy, E, Lilin, T, Nowotny, N, Richardson, J, Zientara, S, Lecollinet, S & Coulpier, M 2013, 'Comparison of the neuropathology induced by two West Nile virus strains', PLoS One, vol. 8, no. 12, e84473. https://doi.org/10.1371/journal.pone.0084473
Donadieu E, Lowenski S, Servely JL, Laloy E, Lilin T, Nowotny N et al. Comparison of the neuropathology induced by two West Nile virus strains. PLoS One. 2013 Dec 18;8(12). e84473. https://doi.org/10.1371/journal.pone.0084473
Donadieu, Emilie ; Lowenski, Steeve ; Servely, Jean Luc ; Laloy, Eve ; Lilin, Thomas ; Nowotny, Norbert ; Richardson, Jennifer ; Zientara, Stéphan ; Lecollinet, Sylvie ; Coulpier, Muriel. / Comparison of the neuropathology induced by two West Nile virus strains. In: PLoS One. 2013 ; Vol. 8, No. 12.
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