Comparative studies on the physiobiochemical, enzymatic, and ionic modifications in salt-tolerant and salt-sensitive citrus rootstocks under NaCl Stress

Rashad M. Balal, Muhammad M. Khan, Muhammad A. Shahid, Neil S. Mattson, Tahira Abbas, Muhammad Ashfaq, Franscisco Garcia-Sanchez, Usman Ghazanfer, Vicente Gimeno, Zafar Iqbal

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

A study was conducted to investigate themorphological, physiobiochemical, enzymatic, and ionic differences among four cultivated citrus (Citrus sp.) rootstocks with different salt tolerances. Two salt-tolerant rootstocks [Rangpur lime (C. limonia) and Rubidoux (C. trifoliata)] and two salt-sensitive rootstocks [Carrizo citrange (Citrus sinensis × C. trifoliata) and Sanchton citrumello (C. trifoliata × C. paradisi)], were subjected to NaCl stress in greenhouse conditions. The 9-month-old plants were exposed to four different NaCl levels (0, 30, 60, or 90mM) in sand culture for 3 months. Plant biomass (fresh weight, dry weight, root length, shoot length, and leaf thickness), physiological attributes [number of stomata, stomatal size, number of epidermal cells, photosynthesis rate, stomatal conductance (gS), water use efficiency, and transpiration rate]. and ion content (Na+, K+, Ca+2, Mg+2, andCl-) were adversely affected by salt stress, but salt-tolerant cultivars were comparatively less affected. Salt stress also enhanced antioxidant enzyme activity (superoxide dismutase, catalase, and peroxidase), particularly in salt-tolerant cultivars. The salt-sensitive cultivars accumulated the greatest content of Na+ and Cl- in their leaves, whereas the salt-tolerant cultivars accumulated the greatest content of Na+ and Cl- in their roots, an adaptation to combat the highly saline conditions. Overall, it was concluded that the salt tolerance of rootstocks is associated with a greater antioxidant enzyme activity and differing accumulation patterns of Na+, K+, Cl-,Mg+2, and Ca+2 in leaves and roots; these can be considered potential indicators of a cultivar's sensitivity to salt stress.

Original languageEnglish
Pages (from-to)86-95
Number of pages10
JournalJournal of the American Society for Horticultural Science
Volume137
Issue number2
Publication statusPublished - 2012

Fingerprint

Citrus
rootstocks
Salts
salts
Poncirus trifoliata
cultivars
salt stress
Citrus limonia
salt tolerance
Salt-Tolerance
enzyme activity
Citroncirus webberi
antioxidants
leaves
Citrus paradisi
Citrus sinensis
Plumbaginaceae
Antioxidants
stomata
water use efficiency

ASJC Scopus subject areas

  • Horticulture
  • Genetics

Cite this

Comparative studies on the physiobiochemical, enzymatic, and ionic modifications in salt-tolerant and salt-sensitive citrus rootstocks under NaCl Stress. / Balal, Rashad M.; Khan, Muhammad M.; Shahid, Muhammad A.; Mattson, Neil S.; Abbas, Tahira; Ashfaq, Muhammad; Garcia-Sanchez, Franscisco; Ghazanfer, Usman; Gimeno, Vicente; Iqbal, Zafar.

In: Journal of the American Society for Horticultural Science, Vol. 137, No. 2, 2012, p. 86-95.

Research output: Contribution to journalArticle

Balal, RM, Khan, MM, Shahid, MA, Mattson, NS, Abbas, T, Ashfaq, M, Garcia-Sanchez, F, Ghazanfer, U, Gimeno, V & Iqbal, Z 2012, 'Comparative studies on the physiobiochemical, enzymatic, and ionic modifications in salt-tolerant and salt-sensitive citrus rootstocks under NaCl Stress', Journal of the American Society for Horticultural Science, vol. 137, no. 2, pp. 86-95.
Balal, Rashad M. ; Khan, Muhammad M. ; Shahid, Muhammad A. ; Mattson, Neil S. ; Abbas, Tahira ; Ashfaq, Muhammad ; Garcia-Sanchez, Franscisco ; Ghazanfer, Usman ; Gimeno, Vicente ; Iqbal, Zafar. / Comparative studies on the physiobiochemical, enzymatic, and ionic modifications in salt-tolerant and salt-sensitive citrus rootstocks under NaCl Stress. In: Journal of the American Society for Horticultural Science. 2012 ; Vol. 137, No. 2. pp. 86-95.
@article{4b6e1a419ce24a3f988bea6376d94b67,
title = "Comparative studies on the physiobiochemical, enzymatic, and ionic modifications in salt-tolerant and salt-sensitive citrus rootstocks under NaCl Stress",
abstract = "A study was conducted to investigate themorphological, physiobiochemical, enzymatic, and ionic differences among four cultivated citrus (Citrus sp.) rootstocks with different salt tolerances. Two salt-tolerant rootstocks [Rangpur lime (C. limonia) and Rubidoux (C. trifoliata)] and two salt-sensitive rootstocks [Carrizo citrange (Citrus sinensis × C. trifoliata) and Sanchton citrumello (C. trifoliata × C. paradisi)], were subjected to NaCl stress in greenhouse conditions. The 9-month-old plants were exposed to four different NaCl levels (0, 30, 60, or 90mM) in sand culture for 3 months. Plant biomass (fresh weight, dry weight, root length, shoot length, and leaf thickness), physiological attributes [number of stomata, stomatal size, number of epidermal cells, photosynthesis rate, stomatal conductance (gS), water use efficiency, and transpiration rate]. and ion content (Na+, K+, Ca+2, Mg+2, andCl-) were adversely affected by salt stress, but salt-tolerant cultivars were comparatively less affected. Salt stress also enhanced antioxidant enzyme activity (superoxide dismutase, catalase, and peroxidase), particularly in salt-tolerant cultivars. The salt-sensitive cultivars accumulated the greatest content of Na+ and Cl- in their leaves, whereas the salt-tolerant cultivars accumulated the greatest content of Na+ and Cl- in their roots, an adaptation to combat the highly saline conditions. Overall, it was concluded that the salt tolerance of rootstocks is associated with a greater antioxidant enzyme activity and differing accumulation patterns of Na+, K+, Cl-,Mg+2, and Ca+2 in leaves and roots; these can be considered potential indicators of a cultivar's sensitivity to salt stress.",
author = "Balal, {Rashad M.} and Khan, {Muhammad M.} and Shahid, {Muhammad A.} and Mattson, {Neil S.} and Tahira Abbas and Muhammad Ashfaq and Franscisco Garcia-Sanchez and Usman Ghazanfer and Vicente Gimeno and Zafar Iqbal",
year = "2012",
language = "English",
volume = "137",
pages = "86--95",
journal = "Journal of the American Society for Horticultural Science",
issn = "0003-1062",
publisher = "American Society for Horticultural Science",
number = "2",

}

TY - JOUR

T1 - Comparative studies on the physiobiochemical, enzymatic, and ionic modifications in salt-tolerant and salt-sensitive citrus rootstocks under NaCl Stress

AU - Balal, Rashad M.

AU - Khan, Muhammad M.

AU - Shahid, Muhammad A.

AU - Mattson, Neil S.

AU - Abbas, Tahira

AU - Ashfaq, Muhammad

AU - Garcia-Sanchez, Franscisco

AU - Ghazanfer, Usman

AU - Gimeno, Vicente

AU - Iqbal, Zafar

PY - 2012

Y1 - 2012

N2 - A study was conducted to investigate themorphological, physiobiochemical, enzymatic, and ionic differences among four cultivated citrus (Citrus sp.) rootstocks with different salt tolerances. Two salt-tolerant rootstocks [Rangpur lime (C. limonia) and Rubidoux (C. trifoliata)] and two salt-sensitive rootstocks [Carrizo citrange (Citrus sinensis × C. trifoliata) and Sanchton citrumello (C. trifoliata × C. paradisi)], were subjected to NaCl stress in greenhouse conditions. The 9-month-old plants were exposed to four different NaCl levels (0, 30, 60, or 90mM) in sand culture for 3 months. Plant biomass (fresh weight, dry weight, root length, shoot length, and leaf thickness), physiological attributes [number of stomata, stomatal size, number of epidermal cells, photosynthesis rate, stomatal conductance (gS), water use efficiency, and transpiration rate]. and ion content (Na+, K+, Ca+2, Mg+2, andCl-) were adversely affected by salt stress, but salt-tolerant cultivars were comparatively less affected. Salt stress also enhanced antioxidant enzyme activity (superoxide dismutase, catalase, and peroxidase), particularly in salt-tolerant cultivars. The salt-sensitive cultivars accumulated the greatest content of Na+ and Cl- in their leaves, whereas the salt-tolerant cultivars accumulated the greatest content of Na+ and Cl- in their roots, an adaptation to combat the highly saline conditions. Overall, it was concluded that the salt tolerance of rootstocks is associated with a greater antioxidant enzyme activity and differing accumulation patterns of Na+, K+, Cl-,Mg+2, and Ca+2 in leaves and roots; these can be considered potential indicators of a cultivar's sensitivity to salt stress.

AB - A study was conducted to investigate themorphological, physiobiochemical, enzymatic, and ionic differences among four cultivated citrus (Citrus sp.) rootstocks with different salt tolerances. Two salt-tolerant rootstocks [Rangpur lime (C. limonia) and Rubidoux (C. trifoliata)] and two salt-sensitive rootstocks [Carrizo citrange (Citrus sinensis × C. trifoliata) and Sanchton citrumello (C. trifoliata × C. paradisi)], were subjected to NaCl stress in greenhouse conditions. The 9-month-old plants were exposed to four different NaCl levels (0, 30, 60, or 90mM) in sand culture for 3 months. Plant biomass (fresh weight, dry weight, root length, shoot length, and leaf thickness), physiological attributes [number of stomata, stomatal size, number of epidermal cells, photosynthesis rate, stomatal conductance (gS), water use efficiency, and transpiration rate]. and ion content (Na+, K+, Ca+2, Mg+2, andCl-) were adversely affected by salt stress, but salt-tolerant cultivars were comparatively less affected. Salt stress also enhanced antioxidant enzyme activity (superoxide dismutase, catalase, and peroxidase), particularly in salt-tolerant cultivars. The salt-sensitive cultivars accumulated the greatest content of Na+ and Cl- in their leaves, whereas the salt-tolerant cultivars accumulated the greatest content of Na+ and Cl- in their roots, an adaptation to combat the highly saline conditions. Overall, it was concluded that the salt tolerance of rootstocks is associated with a greater antioxidant enzyme activity and differing accumulation patterns of Na+, K+, Cl-,Mg+2, and Ca+2 in leaves and roots; these can be considered potential indicators of a cultivar's sensitivity to salt stress.

UR - http://www.scopus.com/inward/record.url?scp=84861007088&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84861007088&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:84861007088

VL - 137

SP - 86

EP - 95

JO - Journal of the American Society for Horticultural Science

JF - Journal of the American Society for Horticultural Science

SN - 0003-1062

IS - 2

ER -