Comparative studies on the physiobiochemical, enzymatic, and ionic modifications in salt-tolerant and salt-sensitive citrus rootstocks under NaCl Stress

Rashad M. Balal, Muhammad M. Khan, Muhammad A. Shahid, Neil S. Mattson, Tahira Abbas, Muhammad Ashfaq, Franscisco Garcia-Sanchez, Usman Ghazanfer, Vicente Gimeno, Zafar Iqbal

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Abstract

A study was conducted to investigate themorphological, physiobiochemical, enzymatic, and ionic differences among four cultivated citrus (Citrus sp.) rootstocks with different salt tolerances. Two salt-tolerant rootstocks [Rangpur lime (C. limonia) and Rubidoux (C. trifoliata)] and two salt-sensitive rootstocks [Carrizo citrange (Citrus sinensis × C. trifoliata) and Sanchton citrumello (C. trifoliata × C. paradisi)], were subjected to NaCl stress in greenhouse conditions. The 9-month-old plants were exposed to four different NaCl levels (0, 30, 60, or 90mM) in sand culture for 3 months. Plant biomass (fresh weight, dry weight, root length, shoot length, and leaf thickness), physiological attributes [number of stomata, stomatal size, number of epidermal cells, photosynthesis rate, stomatal conductance (gS), water use efficiency, and transpiration rate]. and ion content (Na+, K+, Ca+2, Mg+2, andCl-) were adversely affected by salt stress, but salt-tolerant cultivars were comparatively less affected. Salt stress also enhanced antioxidant enzyme activity (superoxide dismutase, catalase, and peroxidase), particularly in salt-tolerant cultivars. The salt-sensitive cultivars accumulated the greatest content of Na+ and Cl- in their leaves, whereas the salt-tolerant cultivars accumulated the greatest content of Na+ and Cl- in their roots, an adaptation to combat the highly saline conditions. Overall, it was concluded that the salt tolerance of rootstocks is associated with a greater antioxidant enzyme activity and differing accumulation patterns of Na+, K+, Cl-,Mg+2, and Ca+2 in leaves and roots; these can be considered potential indicators of a cultivar's sensitivity to salt stress.

Original languageEnglish
Pages (from-to)86-95
Number of pages10
JournalJournal of the American Society for Horticultural Science
Volume137
Issue number2
Publication statusPublished - 2012

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ASJC Scopus subject areas

  • Horticulture
  • Genetics

Cite this

Balal, R. M., Khan, M. M., Shahid, M. A., Mattson, N. S., Abbas, T., Ashfaq, M., Garcia-Sanchez, F., Ghazanfer, U., Gimeno, V., & Iqbal, Z. (2012). Comparative studies on the physiobiochemical, enzymatic, and ionic modifications in salt-tolerant and salt-sensitive citrus rootstocks under NaCl Stress. Journal of the American Society for Horticultural Science, 137(2), 86-95.