Comparative analysis of total phenolics, flavonoid content and antioxidant profile of different date varieties (Phoenix dactylifera L.) From Sultanate of Oman

V. Singh, N. Guizani, M. M. Essa, F. L. Hakkim, M. S. Rahman

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35 Citations (Scopus)

Abstract

The present study investigates the comparative antioxidant ability of the date fruits (DF's) (Phoenix dactylifera) of three major date palm varieties such as Fardh, Khasab and Khalas grown in the Sultanate of Oman at two edible maturation stages, namely Rutab (fresh) and Tamr (dried). Antioxidant ability was assessed by using 2, 2-azinobis (3-ethyl benzo thiazoline-6-sulphonic acid) radical cation (ABTS.+), 2,2-diphenyl-1-picrylhydrazyl (DPPH), Superoxide-radical scavenging (SRSA), ferric reducing/antioxidant power (FRAP assay), Total reducing power ability (TRPA) and Metal chelation assays. The total phenolic content (TPC) and total flavonoid content (TFC) of the DF's were also quantified. The total phenolic content (TPC) of all three varieties at Rutab and Tamr stages ranged from 81-178 and 194-234 mg gallic acid equivalents (GAE)/100 gm of DM and total flavanoid content (TFC) ranged from 19-66 and 25-34 mg catechin equivalents (CEQ)/100 gm of DM respectively. The % inhibition in ABTS assay of all three varieties of DF's at Rutab and Tamr stages ranges from 80-92% and 84-92% respectively and the % inhibition of DF's at Rutab and Tamr stages ranges from 63-65% and 70-73 % respectively observed from DPPH assay. Whereas in SRSA, % inhibition of DF's at Rutab and Tamr stages ranges from 34-43% and 29-44% respectively. The % chelating effect of all three varieties of DF's at Rutab and Tamr stages was 93% and 80-87% respectively. The observed antioxidant ability of DF's may be due to abundant presence of phenolic contents and high electron donating ability to neutralize free radicals. Overall the fardh variety showed significant antioxidant ability and phenolic contents than other varieties at different stages.

Original languageEnglish
Pages (from-to)1063-1070
Number of pages8
JournalInternational Food Research Journal
Volume19
Issue number3
Publication statusPublished - 2012

Fingerprint

Oman
Phoenix dactylifera
dates (fruit)
Flavonoids
flavonoids
Antioxidants
antioxidants
assays
superoxide anion
Superoxides
flavanoids
chelation
sulfonic acid
Phoeniceae
gallic acid
catechin
Gallic Acid
Sulfonic Acids
Catechin
cations

Keywords

  • Abts
  • Antioxidant Activity
  • Date Palm Fruits
  • Dpph
  • Flavonoids
  • Reducing Power
  • Superoxide Anion

ASJC Scopus subject areas

  • Food Science

Cite this

@article{396d5dccd6764d9fbc5ebcbf14e8c228,
title = "Comparative analysis of total phenolics, flavonoid content and antioxidant profile of different date varieties (Phoenix dactylifera L.) From Sultanate of Oman",
abstract = "The present study investigates the comparative antioxidant ability of the date fruits (DF's) (Phoenix dactylifera) of three major date palm varieties such as Fardh, Khasab and Khalas grown in the Sultanate of Oman at two edible maturation stages, namely Rutab (fresh) and Tamr (dried). Antioxidant ability was assessed by using 2, 2-azinobis (3-ethyl benzo thiazoline-6-sulphonic acid) radical cation (ABTS.+), 2,2-diphenyl-1-picrylhydrazyl (DPPH), Superoxide-radical scavenging (SRSA), ferric reducing/antioxidant power (FRAP assay), Total reducing power ability (TRPA) and Metal chelation assays. The total phenolic content (TPC) and total flavonoid content (TFC) of the DF's were also quantified. The total phenolic content (TPC) of all three varieties at Rutab and Tamr stages ranged from 81-178 and 194-234 mg gallic acid equivalents (GAE)/100 gm of DM and total flavanoid content (TFC) ranged from 19-66 and 25-34 mg catechin equivalents (CEQ)/100 gm of DM respectively. The {\%} inhibition in ABTS assay of all three varieties of DF's at Rutab and Tamr stages ranges from 80-92{\%} and 84-92{\%} respectively and the {\%} inhibition of DF's at Rutab and Tamr stages ranges from 63-65{\%} and 70-73 {\%} respectively observed from DPPH assay. Whereas in SRSA, {\%} inhibition of DF's at Rutab and Tamr stages ranges from 34-43{\%} and 29-44{\%} respectively. The {\%} chelating effect of all three varieties of DF's at Rutab and Tamr stages was 93{\%} and 80-87{\%} respectively. The observed antioxidant ability of DF's may be due to abundant presence of phenolic contents and high electron donating ability to neutralize free radicals. Overall the fardh variety showed significant antioxidant ability and phenolic contents than other varieties at different stages.",
keywords = "Abts, Antioxidant Activity, Date Palm Fruits, Dpph, Flavonoids, Reducing Power, Superoxide Anion",
author = "V. Singh and N. Guizani and Essa, {M. M.} and Hakkim, {F. L.} and Rahman, {M. S.}",
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T1 - Comparative analysis of total phenolics, flavonoid content and antioxidant profile of different date varieties (Phoenix dactylifera L.) From Sultanate of Oman

AU - Singh, V.

AU - Guizani, N.

AU - Essa, M. M.

AU - Hakkim, F. L.

AU - Rahman, M. S.

PY - 2012

Y1 - 2012

N2 - The present study investigates the comparative antioxidant ability of the date fruits (DF's) (Phoenix dactylifera) of three major date palm varieties such as Fardh, Khasab and Khalas grown in the Sultanate of Oman at two edible maturation stages, namely Rutab (fresh) and Tamr (dried). Antioxidant ability was assessed by using 2, 2-azinobis (3-ethyl benzo thiazoline-6-sulphonic acid) radical cation (ABTS.+), 2,2-diphenyl-1-picrylhydrazyl (DPPH), Superoxide-radical scavenging (SRSA), ferric reducing/antioxidant power (FRAP assay), Total reducing power ability (TRPA) and Metal chelation assays. The total phenolic content (TPC) and total flavonoid content (TFC) of the DF's were also quantified. The total phenolic content (TPC) of all three varieties at Rutab and Tamr stages ranged from 81-178 and 194-234 mg gallic acid equivalents (GAE)/100 gm of DM and total flavanoid content (TFC) ranged from 19-66 and 25-34 mg catechin equivalents (CEQ)/100 gm of DM respectively. The % inhibition in ABTS assay of all three varieties of DF's at Rutab and Tamr stages ranges from 80-92% and 84-92% respectively and the % inhibition of DF's at Rutab and Tamr stages ranges from 63-65% and 70-73 % respectively observed from DPPH assay. Whereas in SRSA, % inhibition of DF's at Rutab and Tamr stages ranges from 34-43% and 29-44% respectively. The % chelating effect of all three varieties of DF's at Rutab and Tamr stages was 93% and 80-87% respectively. The observed antioxidant ability of DF's may be due to abundant presence of phenolic contents and high electron donating ability to neutralize free radicals. Overall the fardh variety showed significant antioxidant ability and phenolic contents than other varieties at different stages.

AB - The present study investigates the comparative antioxidant ability of the date fruits (DF's) (Phoenix dactylifera) of three major date palm varieties such as Fardh, Khasab and Khalas grown in the Sultanate of Oman at two edible maturation stages, namely Rutab (fresh) and Tamr (dried). Antioxidant ability was assessed by using 2, 2-azinobis (3-ethyl benzo thiazoline-6-sulphonic acid) radical cation (ABTS.+), 2,2-diphenyl-1-picrylhydrazyl (DPPH), Superoxide-radical scavenging (SRSA), ferric reducing/antioxidant power (FRAP assay), Total reducing power ability (TRPA) and Metal chelation assays. The total phenolic content (TPC) and total flavonoid content (TFC) of the DF's were also quantified. The total phenolic content (TPC) of all three varieties at Rutab and Tamr stages ranged from 81-178 and 194-234 mg gallic acid equivalents (GAE)/100 gm of DM and total flavanoid content (TFC) ranged from 19-66 and 25-34 mg catechin equivalents (CEQ)/100 gm of DM respectively. The % inhibition in ABTS assay of all three varieties of DF's at Rutab and Tamr stages ranges from 80-92% and 84-92% respectively and the % inhibition of DF's at Rutab and Tamr stages ranges from 63-65% and 70-73 % respectively observed from DPPH assay. Whereas in SRSA, % inhibition of DF's at Rutab and Tamr stages ranges from 34-43% and 29-44% respectively. The % chelating effect of all three varieties of DF's at Rutab and Tamr stages was 93% and 80-87% respectively. The observed antioxidant ability of DF's may be due to abundant presence of phenolic contents and high electron donating ability to neutralize free radicals. Overall the fardh variety showed significant antioxidant ability and phenolic contents than other varieties at different stages.

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KW - Dpph

KW - Flavonoids

KW - Reducing Power

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