Comparative analysis of gene expression of Ty-1 hybrid and non-hybrid tomatoes exposed to tomato yellow leaf curl virus strains

Muhammad Shafiq Shahid, Junji Kimbara, Ayumu Onozato, Keiko T. Natsuaki, Masato Ikegami

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Tomato (S. lycopersicum) is an economically important vegetable food crop and threatened by TYLCV, a devastating pathogen throughout the world. In the present study, we analyzed differential gene expression in response to TYLCV-IL and TYLCV-Mld (Begomovirus, Geminiviridae) viral strains infection in the Ty-1 tomato hybrids (Esns1) and non-hybrid tomato (P1788) accessions. cDNA microarray hybridization was used to detect a group of genes that were differentially expressed in Esns1infected with TYLCV strains at 18 days post inoculation. The microarray analysis recognized significantly changed expression of different gene targets. Additional analysis confirmed that 43 genes were significantly up-regulated and 7 were down-regulated in the Esns1against compared to P1788 with TYLCV strains. Overall, major differences in gene expression were characterized by major physiological functions containing pathogen, defense, photosynthesis, cell wall or growth, metabolic, stress, biosynthetic and signaling-related responses. This is the first comparative genome-wide transcriptional study of the expression of Esns1plants depicting diverse responses to biotic stress induced by TYLCV-IL and TYLCV-Mld. Additionally, this study also provides sufficient knowledge into the identification of key defense-related genes in tomato for TYLCV disease management.

Original languageEnglish
Pages (from-to)819-825
Number of pages7
JournalAustralian Journal of Crop Science
Volume9
Issue number9
Publication statusPublished - 2015

Fingerprint

Tomato yellow leaf curl virus
tomatoes
gene expression
taxonomic keys
Geminiviridae
Begomovirus
microarray technology
genes
pathogens
food crops
vegetable crops
biotic stress
cell growth
disease control
cell walls
photosynthesis
genome

Keywords

  • Begomovirus
  • Gene expression
  • Microarrays
  • Tomato
  • TYLCV

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Comparative analysis of gene expression of Ty-1 hybrid and non-hybrid tomatoes exposed to tomato yellow leaf curl virus strains. / Shahid, Muhammad Shafiq; Kimbara, Junji; Onozato, Ayumu; Natsuaki, Keiko T.; Ikegami, Masato.

In: Australian Journal of Crop Science, Vol. 9, No. 9, 2015, p. 819-825.

Research output: Contribution to journalArticle

@article{6954c395088f48f5b77080d46c96ec66,
title = "Comparative analysis of gene expression of Ty-1 hybrid and non-hybrid tomatoes exposed to tomato yellow leaf curl virus strains",
abstract = "Tomato (S. lycopersicum) is an economically important vegetable food crop and threatened by TYLCV, a devastating pathogen throughout the world. In the present study, we analyzed differential gene expression in response to TYLCV-IL and TYLCV-Mld (Begomovirus, Geminiviridae) viral strains infection in the Ty-1 tomato hybrids (Esns1) and non-hybrid tomato (P1788) accessions. cDNA microarray hybridization was used to detect a group of genes that were differentially expressed in Esns1infected with TYLCV strains at 18 days post inoculation. The microarray analysis recognized significantly changed expression of different gene targets. Additional analysis confirmed that 43 genes were significantly up-regulated and 7 were down-regulated in the Esns1against compared to P1788 with TYLCV strains. Overall, major differences in gene expression were characterized by major physiological functions containing pathogen, defense, photosynthesis, cell wall or growth, metabolic, stress, biosynthetic and signaling-related responses. This is the first comparative genome-wide transcriptional study of the expression of Esns1plants depicting diverse responses to biotic stress induced by TYLCV-IL and TYLCV-Mld. Additionally, this study also provides sufficient knowledge into the identification of key defense-related genes in tomato for TYLCV disease management.",
keywords = "Begomovirus, Gene expression, Microarrays, Tomato, TYLCV",
author = "Shahid, {Muhammad Shafiq} and Junji Kimbara and Ayumu Onozato and Natsuaki, {Keiko T.} and Masato Ikegami",
year = "2015",
language = "English",
volume = "9",
pages = "819--825",
journal = "Australian Journal of Crop Science",
issn = "1835-2693",
publisher = "Southern Cross Publishing and Printing Pty Ltd",
number = "9",

}

TY - JOUR

T1 - Comparative analysis of gene expression of Ty-1 hybrid and non-hybrid tomatoes exposed to tomato yellow leaf curl virus strains

AU - Shahid, Muhammad Shafiq

AU - Kimbara, Junji

AU - Onozato, Ayumu

AU - Natsuaki, Keiko T.

AU - Ikegami, Masato

PY - 2015

Y1 - 2015

N2 - Tomato (S. lycopersicum) is an economically important vegetable food crop and threatened by TYLCV, a devastating pathogen throughout the world. In the present study, we analyzed differential gene expression in response to TYLCV-IL and TYLCV-Mld (Begomovirus, Geminiviridae) viral strains infection in the Ty-1 tomato hybrids (Esns1) and non-hybrid tomato (P1788) accessions. cDNA microarray hybridization was used to detect a group of genes that were differentially expressed in Esns1infected with TYLCV strains at 18 days post inoculation. The microarray analysis recognized significantly changed expression of different gene targets. Additional analysis confirmed that 43 genes were significantly up-regulated and 7 were down-regulated in the Esns1against compared to P1788 with TYLCV strains. Overall, major differences in gene expression were characterized by major physiological functions containing pathogen, defense, photosynthesis, cell wall or growth, metabolic, stress, biosynthetic and signaling-related responses. This is the first comparative genome-wide transcriptional study of the expression of Esns1plants depicting diverse responses to biotic stress induced by TYLCV-IL and TYLCV-Mld. Additionally, this study also provides sufficient knowledge into the identification of key defense-related genes in tomato for TYLCV disease management.

AB - Tomato (S. lycopersicum) is an economically important vegetable food crop and threatened by TYLCV, a devastating pathogen throughout the world. In the present study, we analyzed differential gene expression in response to TYLCV-IL and TYLCV-Mld (Begomovirus, Geminiviridae) viral strains infection in the Ty-1 tomato hybrids (Esns1) and non-hybrid tomato (P1788) accessions. cDNA microarray hybridization was used to detect a group of genes that were differentially expressed in Esns1infected with TYLCV strains at 18 days post inoculation. The microarray analysis recognized significantly changed expression of different gene targets. Additional analysis confirmed that 43 genes were significantly up-regulated and 7 were down-regulated in the Esns1against compared to P1788 with TYLCV strains. Overall, major differences in gene expression were characterized by major physiological functions containing pathogen, defense, photosynthesis, cell wall or growth, metabolic, stress, biosynthetic and signaling-related responses. This is the first comparative genome-wide transcriptional study of the expression of Esns1plants depicting diverse responses to biotic stress induced by TYLCV-IL and TYLCV-Mld. Additionally, this study also provides sufficient knowledge into the identification of key defense-related genes in tomato for TYLCV disease management.

KW - Begomovirus

KW - Gene expression

KW - Microarrays

KW - Tomato

KW - TYLCV

UR - http://www.scopus.com/inward/record.url?scp=84945133215&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84945133215&partnerID=8YFLogxK

M3 - Article

VL - 9

SP - 819

EP - 825

JO - Australian Journal of Crop Science

JF - Australian Journal of Crop Science

SN - 1835-2693

IS - 9

ER -