Colocalisation of human immunodeficiency virus and human cytomegalovirus infection in brain autopsy tissue from AIDS patients

I. M. Balluz, M. A. Farrell, E. Kay, M. J. Staunton, J. N. Keating, O. Sheils, S. L. Cosby, M. J.E.M.F. Mabruk, B. J. Sheahan, G. J. Atkins

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We have examined 26 human AIDS brains obtained at post mortem for infection by human immunodeficiency virus (HIV) and human cytomegalovirus (HCMV), and for dual infection of cells by both viruses. The techniques used were enzyme-linked immunocytochemistry for HCMV and in situ hybridisation using a cDNA probe for HIV. Using these techniques, HCMV infection was detected in 14 brains, HIV infection in 14 brains, and coinfection with HIV and HCMV in 7 brains. Four caes of dual HIV/HCMV infection were found where no colocalisation could be detected. In randomly chosen dually infected areas 19.2% of infected cells were coinfected with both viruses. Although cells identified morphologically as macrophages were the most common infected cell type, astrocytes and neurons were both singly and doubly infected with HIV and HCMV. Complete clinical data were available for 4 of the 7 cases with coinfection and each had AIDS dementia complex.

Original languageEnglish
Pages (from-to)133-138
Number of pages6
JournalIrish Journal of Medical Science
Volume165
Issue number2
Publication statusPublished - Apr 1996

Fingerprint

Cytomegalovirus Infections
Autopsy
Acquired Immunodeficiency Syndrome
HIV
Brain
Cytomegalovirus
Coinfection
AIDS Dementia Complex
Viruses
Virus Diseases
Astrocytes
In Situ Hybridization
Complementary DNA
Immunohistochemistry
Macrophages
Neurons
Enzymes
Infection

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Balluz, I. M., Farrell, M. A., Kay, E., Staunton, M. J., Keating, J. N., Sheils, O., ... Atkins, G. J. (1996). Colocalisation of human immunodeficiency virus and human cytomegalovirus infection in brain autopsy tissue from AIDS patients. Irish Journal of Medical Science, 165(2), 133-138.

Colocalisation of human immunodeficiency virus and human cytomegalovirus infection in brain autopsy tissue from AIDS patients. / Balluz, I. M.; Farrell, M. A.; Kay, E.; Staunton, M. J.; Keating, J. N.; Sheils, O.; Cosby, S. L.; Mabruk, M. J.E.M.F.; Sheahan, B. J.; Atkins, G. J.

In: Irish Journal of Medical Science, Vol. 165, No. 2, 04.1996, p. 133-138.

Research output: Contribution to journalArticle

Balluz, IM, Farrell, MA, Kay, E, Staunton, MJ, Keating, JN, Sheils, O, Cosby, SL, Mabruk, MJEMF, Sheahan, BJ & Atkins, GJ 1996, 'Colocalisation of human immunodeficiency virus and human cytomegalovirus infection in brain autopsy tissue from AIDS patients', Irish Journal of Medical Science, vol. 165, no. 2, pp. 133-138.
Balluz, I. M. ; Farrell, M. A. ; Kay, E. ; Staunton, M. J. ; Keating, J. N. ; Sheils, O. ; Cosby, S. L. ; Mabruk, M. J.E.M.F. ; Sheahan, B. J. ; Atkins, G. J. / Colocalisation of human immunodeficiency virus and human cytomegalovirus infection in brain autopsy tissue from AIDS patients. In: Irish Journal of Medical Science. 1996 ; Vol. 165, No. 2. pp. 133-138.
@article{525f48ade08a4cafae843d8f4e6926a5,
title = "Colocalisation of human immunodeficiency virus and human cytomegalovirus infection in brain autopsy tissue from AIDS patients",
abstract = "We have examined 26 human AIDS brains obtained at post mortem for infection by human immunodeficiency virus (HIV) and human cytomegalovirus (HCMV), and for dual infection of cells by both viruses. The techniques used were enzyme-linked immunocytochemistry for HCMV and in situ hybridisation using a cDNA probe for HIV. Using these techniques, HCMV infection was detected in 14 brains, HIV infection in 14 brains, and coinfection with HIV and HCMV in 7 brains. Four caes of dual HIV/HCMV infection were found where no colocalisation could be detected. In randomly chosen dually infected areas 19.2{\%} of infected cells were coinfected with both viruses. Although cells identified morphologically as macrophages were the most common infected cell type, astrocytes and neurons were both singly and doubly infected with HIV and HCMV. Complete clinical data were available for 4 of the 7 cases with coinfection and each had AIDS dementia complex.",
author = "Balluz, {I. M.} and Farrell, {M. A.} and E. Kay and Staunton, {M. J.} and Keating, {J. N.} and O. Sheils and Cosby, {S. L.} and Mabruk, {M. J.E.M.F.} and Sheahan, {B. J.} and Atkins, {G. J.}",
year = "1996",
month = "4",
language = "English",
volume = "165",
pages = "133--138",
journal = "The Dublin Journal of Medical and Chemical Science",
issn = "0332-1029",
publisher = "Springer London",
number = "2",

}

TY - JOUR

T1 - Colocalisation of human immunodeficiency virus and human cytomegalovirus infection in brain autopsy tissue from AIDS patients

AU - Balluz, I. M.

AU - Farrell, M. A.

AU - Kay, E.

AU - Staunton, M. J.

AU - Keating, J. N.

AU - Sheils, O.

AU - Cosby, S. L.

AU - Mabruk, M. J.E.M.F.

AU - Sheahan, B. J.

AU - Atkins, G. J.

PY - 1996/4

Y1 - 1996/4

N2 - We have examined 26 human AIDS brains obtained at post mortem for infection by human immunodeficiency virus (HIV) and human cytomegalovirus (HCMV), and for dual infection of cells by both viruses. The techniques used were enzyme-linked immunocytochemistry for HCMV and in situ hybridisation using a cDNA probe for HIV. Using these techniques, HCMV infection was detected in 14 brains, HIV infection in 14 brains, and coinfection with HIV and HCMV in 7 brains. Four caes of dual HIV/HCMV infection were found where no colocalisation could be detected. In randomly chosen dually infected areas 19.2% of infected cells were coinfected with both viruses. Although cells identified morphologically as macrophages were the most common infected cell type, astrocytes and neurons were both singly and doubly infected with HIV and HCMV. Complete clinical data were available for 4 of the 7 cases with coinfection and each had AIDS dementia complex.

AB - We have examined 26 human AIDS brains obtained at post mortem for infection by human immunodeficiency virus (HIV) and human cytomegalovirus (HCMV), and for dual infection of cells by both viruses. The techniques used were enzyme-linked immunocytochemistry for HCMV and in situ hybridisation using a cDNA probe for HIV. Using these techniques, HCMV infection was detected in 14 brains, HIV infection in 14 brains, and coinfection with HIV and HCMV in 7 brains. Four caes of dual HIV/HCMV infection were found where no colocalisation could be detected. In randomly chosen dually infected areas 19.2% of infected cells were coinfected with both viruses. Although cells identified morphologically as macrophages were the most common infected cell type, astrocytes and neurons were both singly and doubly infected with HIV and HCMV. Complete clinical data were available for 4 of the 7 cases with coinfection and each had AIDS dementia complex.

UR - http://www.scopus.com/inward/record.url?scp=15844406930&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=15844406930&partnerID=8YFLogxK

M3 - Article

VL - 165

SP - 133

EP - 138

JO - The Dublin Journal of Medical and Chemical Science

JF - The Dublin Journal of Medical and Chemical Science

SN - 0332-1029

IS - 2

ER -