Cloning of AFLP markers linked to resistance to Peronosclerospora sorghi in maize

H. Agrama, S. Houssin, M. Tarek

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Genetic mapping of resistance genes for sorghum downy mildew (SDM) in maize revealed multiple-locus inheritance. A combination of AFLP (amplified fragment length polymorphism) technique with bulked segregant analysis (BSA) was applied to map the genes involved in the resistance to SDM (Peronosclerospora sorghi) in a recombinant inbred population. Three AFLP markers were identified and mapped to chromosomes 1 and 9, in regions previously associated with SDM resistance. One other AFLP marker was found to be associated with disease susceptibility but could not be linked to any chromosome. These four AFLP fragments were isolated, cloned and sequenced. A BLAST search of the GenBank database showed that none of these four sequences was closely related to resistance genes that have been reported previously. Sequence-characterized amplified regions (SCARs) were produced and used to assess the presence of SDM resistance genes and characterize specific genotypes. These markers may be useful in marker-assisted breeding programs.

Original languageEnglish
Pages (from-to)814-819
Number of pages6
JournalMolecular Genetics and Genomics
Volume267
Issue number6
DOIs
Publication statusPublished - 2002

Fingerprint

Sorghum
Zea mays
Organism Cloning
Genes
Chromosomes, Human, Pair 9
Chromosome Mapping
Chromosomes, Human, Pair 1
Disease Susceptibility
Nucleic Acid Databases
Breeding
Chromosomes
Genotype
Databases
Population

Keywords

  • Bulked segregant analysis
  • DNA sequence
  • Resistance gene
  • Sequence-characterized amplified region (SCAR)
  • Sorghum downy mildew

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Cloning of AFLP markers linked to resistance to Peronosclerospora sorghi in maize. / Agrama, H.; Houssin, S.; Tarek, M.

In: Molecular Genetics and Genomics, Vol. 267, No. 6, 2002, p. 814-819.

Research output: Contribution to journalArticle

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