Abstract
Genetic mapping of resistance genes for sorghum downy mildew (SDM) in maize revealed multiple-locus inheritance. A combination of AFLP (amplified fragment length polymorphism) technique with bulked segregant analysis (BSA) was applied to map the genes involved in the resistance to SDM (Peronosclerospora sorghi) in a recombinant inbred population. Three AFLP markers were identified and mapped to chromosomes 1 and 9, in regions previously associated with SDM resistance. One other AFLP marker was found to be associated with disease susceptibility but could not be linked to any chromosome. These four AFLP fragments were isolated, cloned and sequenced. A BLAST search of the GenBank database showed that none of these four sequences was closely related to resistance genes that have been reported previously. Sequence-characterized amplified regions (SCARs) were produced and used to assess the presence of SDM resistance genes and characterize specific genotypes. These markers may be useful in marker-assisted breeding programs.
| Original language | English |
|---|---|
| Pages (from-to) | 814-819 |
| Number of pages | 6 |
| Journal | Molecular Genetics and Genomics |
| Volume | 267 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2002 |
| Externally published | Yes |
Keywords
- Bulked segregant analysis
- DNA sequence
- Resistance gene
- Sequence-characterized amplified region (SCAR)
- Sorghum downy mildew
ASJC Scopus subject areas
- Molecular Biology
- Genetics