Cloning and expression of the inorganic pyrophosphatase gene from the amino acid producer Brevibacterium lactofermentum ATCC 13869

Angelina Ramos, Sirin A I Adham, José A. Gil

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A 20-kDa Brevibacterium lactofermentum protein was detected when purifying the His-tagged FtsZBL. The protein was identified by matrix-assisted laser desorption/ionisation time of flight as the inorganic pyrophosphatase encoded by the ppa gene, which is present as a single copy in the genome of Corynebacterium glutamicum. The ppa gene was cloned from B. lactofermentum chromosomal DNA by polymerase chain reaction; it seemed to be an essential gene and it might represent an attractive target for drug discovery. The cloned ppa gene complemented a ppa- Escherichia coli mutant and a ppa-gfp gene fusion revealed that the gene product mainly accumulated at the cell poles in both E. coli and B. lactofermentum.

Original languageEnglish
Pages (from-to)85-92
Number of pages8
JournalFEMS Microbiology Letters
Volume225
Issue number1
DOIs
Publication statusPublished - Aug 8 2003

Fingerprint

Inorganic Pyrophosphatase
Brevibacterium
Organism Cloning
Amino Acids
Genes
Corynebacterium glutamicum
Escherichia coli
Gene Fusion
Essential Genes
DNA-Directed DNA Polymerase
Drug Discovery
Proteins
Lasers
Genome
Polymerase Chain Reaction

Keywords

  • Cell division
  • Corynebacterium
  • DivIVA
  • FtsZ
  • Green fluorescence protein
  • Inorganic pyrophosphatase

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Cloning and expression of the inorganic pyrophosphatase gene from the amino acid producer Brevibacterium lactofermentum ATCC 13869. / Ramos, Angelina; Adham, Sirin A I; Gil, José A.

In: FEMS Microbiology Letters, Vol. 225, No. 1, 08.08.2003, p. 85-92.

Research output: Contribution to journalArticle

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