Abstract
A 20-kDa Brevibacterium lactofermentum protein was detected when purifying the His-tagged FtsZBL. The protein was identified by matrix-assisted laser desorption/ionisation time of flight as the inorganic pyrophosphatase encoded by the ppa gene, which is present as a single copy in the genome of Corynebacterium glutamicum. The ppa gene was cloned from B. lactofermentum chromosomal DNA by polymerase chain reaction; it seemed to be an essential gene and it might represent an attractive target for drug discovery. The cloned ppa gene complemented a ppa- Escherichia coli mutant and a ppa-gfp gene fusion revealed that the gene product mainly accumulated at the cell poles in both E. coli and B. lactofermentum.
Original language | English |
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Pages (from-to) | 85-92 |
Number of pages | 8 |
Journal | FEMS Microbiology Letters |
Volume | 225 |
Issue number | 1 |
DOIs | |
Publication status | Published - Aug 8 2003 |
Externally published | Yes |
Keywords
- Cell division
- Corynebacterium
- DivIVA
- FtsZ
- Green fluorescence protein
- Inorganic pyrophosphatase
ASJC Scopus subject areas
- Microbiology
- Molecular Biology
- Genetics