A sequential injection (SI) method was developed for the determination of chlorpheniramine (CPA), based on the reaction of this drug with tris(1,10-phenanthroline)-ruthenium(II) [Ru(phen)32+] and peroxydisulphate (S2O82-) in the presence of light. The instrumental set-up utilized a syringe pump and a multiposition valve to aspirate the reagents [Ru(phen)32+ and S 2O82-] and a peristaltic pump to propel the sample. The experimental conditions affecting the chemiluminescence reaction were systematically optimized, using the univariate approach. Under the optimum conditions linear calibration curves of 0.1-10 μg/ml were obtained. The detection limit was 0.04 μg/ml and the relative standard deviation (RSD) was always < 5%. The procedure was applied to the analysis of CPA in pharmaceutical products and was found to be free from interferences from concomitants usually present in these preparations.
ASJC Scopus subject areas
- Chemistry (miscellaneous)