Chemical constituents and antioxidant activity of fresh leaves of Psidium guajava cultivated in Pakistan

Sabira Begum, Syed Nawazish Ali, Saima Tauseef, Syed Tahir Ali, Syed Imran Hassan, Bina Shaheen Siddiqui, Aqeel Ahmad

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The in vitro antioxidant activities of the methanol extract of fresh leaves of Psidium guajava cultivated in Pakistan and its different fractions were evaluated using 1,1-diphenyl-2- picrylhydrazyl (DPPH) free radical scavenging assay. The methanol extract, main ethyl acetate fraction and its polar sub fraction showed high free radical scavenging activity with EC50 11.72, 11.72 and 46.8 μg/mL respectively. The first two values are comparable with that of reference compound ascorbic acid (EC50 9.4 μg/mL). The known antioxidants gallic acid (1), methyl ferulate (2) and methyl p-E-coumarate (3) were isolated from the ethyl acetate insoluble fraction. Their structures were identified by mass, 1H- and 13C-NMR spectroscopy. Compounds 2 and 3 are reported for the first time from the genus Psidium.

Original languageEnglish
Pages (from-to)119-122
Number of pages4
JournalJournal of the Chemical Society of Pakistan
Issue number1
Publication statusPublished - 2014



  • DPPH scavenging activity
  • Gallic acid
  • Methyl ferulate
  • Methyl p-E-coumarate
  • Psidium guajava
  • Reactive oxygen species

ASJC Scopus subject areas

  • Chemistry(all)

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