Characterization of Tomato yellow leaf curl virus and associated alphasatellite infecting Cucurbita maxima in Japan

M. S. Shahid, M. Ikegami, R. W. Briddon, K. T. Natsuaki

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Samples of Cucurbita maxima plants with foliar yellowing and curling symptoms were collected in Komae City (Japan) in 2011 and shown by Southern blot hybridization to be associated with a begomovirus. Rolling circle amplification and PCR-mediated amplification with specific primers showed the presence of a begomovirus and an alphasatellite, but neither a DNA-B component or a betasatellite. Sequence analysis of full-length clones showed the virus to be an isolate of the Israel strain of Tomato yellow leaf curl virus and the alphasatellite Sida yellow vein China alphasatellite. This is the first identification of these components in a cucurbit in Japan.

Original languageEnglish
Pages (from-to)92-95
Number of pages4
JournalJournal of General Plant Pathology
Volume81
Issue number1
DOIs
Publication statusPublished - 2014

Fingerprint

Tomato yellow leaf curl virus
Begomovirus
Cucurbita maxima
B-DNA
Japan
Sida
Cucurbitaceae
plant veins
Southern blotting
Israel
signs and symptoms (plants)
hybridization
sequence analysis
clones
viruses
China
sampling

Keywords

  • Alphasatellite
  • Begomovirus
  • Cucurbita maxima
  • Rolling circle amplification

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science

Cite this

Characterization of Tomato yellow leaf curl virus and associated alphasatellite infecting Cucurbita maxima in Japan. / Shahid, M. S.; Ikegami, M.; Briddon, R. W.; Natsuaki, K. T.

In: Journal of General Plant Pathology, Vol. 81, No. 1, 2014, p. 92-95.

Research output: Contribution to journalArticle

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