Characterization of Tomato yellow leaf curl virus and associated alphasatellite infecting Cucurbita maxima in Japan

M. S. Shahid; M. Ikegami; R. W. Briddon; K. T. Natsuaki

doi:10.1007/s10327-014-0563-x

Characterization of Tomato yellow leaf curl virus and associated alphasatellite infecting Cucurbita maxima in Japan

M. S. Shahid^*, M. Ikegami, R. W. Briddon, K. T. Natsuaki

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Samples of Cucurbita maxima plants with foliar yellowing and curling symptoms were collected in Komae City (Japan) in 2011 and shown by Southern blot hybridization to be associated with a begomovirus. Rolling circle amplification and PCR-mediated amplification with specific primers showed the presence of a begomovirus and an alphasatellite, but neither a DNA-B component or a betasatellite. Sequence analysis of full-length clones showed the virus to be an isolate of the Israel strain of Tomato yellow leaf curl virus and the alphasatellite Sida yellow vein China alphasatellite. This is the first identification of these components in a cucurbit in Japan.

Original language	English
Pages (from-to)	92-95
Number of pages	4
Journal	Journal of General Plant Pathology
Volume	81
Issue number	1
DOIs	https://doi.org/10.1007/s10327-014-0563-x
Publication status	Published - Jan 2014
Externally published	Yes

Keywords

Alphasatellite
Begomovirus
Cucurbita maxima
Rolling circle amplification

ASJC Scopus subject areas

Agronomy and Crop Science
Plant Science

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10.1007/s10327-014-0563-x

Cite this

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title = "Characterization of Tomato yellow leaf curl virus and associated alphasatellite infecting Cucurbita maxima in Japan",

abstract = "Samples of Cucurbita maxima plants with foliar yellowing and curling symptoms were collected in Komae City (Japan) in 2011 and shown by Southern blot hybridization to be associated with a begomovirus. Rolling circle amplification and PCR-mediated amplification with specific primers showed the presence of a begomovirus and an alphasatellite, but neither a DNA-B component or a betasatellite. Sequence analysis of full-length clones showed the virus to be an isolate of the Israel strain of Tomato yellow leaf curl virus and the alphasatellite Sida yellow vein China alphasatellite. This is the first identification of these components in a cucurbit in Japan.",

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doi = "10.1007/s10327-014-0563-x",

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T1 - Characterization of Tomato yellow leaf curl virus and associated alphasatellite infecting Cucurbita maxima in Japan

AU - Shahid, M. S.

AU - Ikegami, M.

AU - Briddon, R. W.

AU - Natsuaki, K. T.

PY - 2014/1

Y1 - 2014/1

N2 - Samples of Cucurbita maxima plants with foliar yellowing and curling symptoms were collected in Komae City (Japan) in 2011 and shown by Southern blot hybridization to be associated with a begomovirus. Rolling circle amplification and PCR-mediated amplification with specific primers showed the presence of a begomovirus and an alphasatellite, but neither a DNA-B component or a betasatellite. Sequence analysis of full-length clones showed the virus to be an isolate of the Israel strain of Tomato yellow leaf curl virus and the alphasatellite Sida yellow vein China alphasatellite. This is the first identification of these components in a cucurbit in Japan.

AB - Samples of Cucurbita maxima plants with foliar yellowing and curling symptoms were collected in Komae City (Japan) in 2011 and shown by Southern blot hybridization to be associated with a begomovirus. Rolling circle amplification and PCR-mediated amplification with specific primers showed the presence of a begomovirus and an alphasatellite, but neither a DNA-B component or a betasatellite. Sequence analysis of full-length clones showed the virus to be an isolate of the Israel strain of Tomato yellow leaf curl virus and the alphasatellite Sida yellow vein China alphasatellite. This is the first identification of these components in a cucurbit in Japan.

KW - Alphasatellite

KW - Begomovirus

KW - Cucurbita maxima

KW - Rolling circle amplification

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Characterization of Tomato yellow leaf curl virus and associated alphasatellite infecting Cucurbita maxima in Japan

Abstract

Keywords

ASJC Scopus subject areas

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