Characterization of proteolytic bacteria from the Aleutian deep-sea and their proteases

Hairong Xiong, Linsheng Song, Ying Xu, Man Yee Tsoi, Sergey Dobretsov, Pei Yuan Qian

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Six deep-sea proteolytic bacteria taken from Aleutian margin sediments were screened; one of them produced a cold-adapted neutral halophilic protease. These bacteria belong to Pseudoalteromonas spp., which were identified by the 16S rDNA sequence. Of the six proteases produced, two were neutral cold-adapted proteases that showed their optimal activity at pH 7-8 and at temperature close to 35°C, and the other four were alkaline proteases that showed their optimal activity at pH 9 and at temperature of 40-45°C. The neutral cold-adapted protease E1 showed its optimal activity at a sodium chloride concentration of 2 M, whereas the activity of the other five proteases decreased at elevated sodium chloride concentrations. Protease E1 was purified to electrophoretic homogeneity and its molecular mass was 34 kDa, as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The molecular weight of protease E1 was determined to be 32,411 Da by mass spectrometric analysis. Phenylmethyl sulfonylfluoride (PMSF) did not inhibit the activity of this protease, whereas it was partially inhibited by ethylenediaminetetra-acetic acid sodium salt (EDTA-Na). De novo amino acid sequencing proved protease E1 to be a novel protein.

Original languageEnglish
Pages (from-to)63-71
Number of pages9
JournalJournal of Industrial Microbiology and Biotechnology
Volume34
Issue number1
DOIs
Publication statusPublished - Jan 2007

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Oceans and Seas
Bacteria
Peptide Hydrolases
Sodium Chloride
Sodium chloride
Pseudoalteromonas
Temperature
Protein Sequence Analysis
Molecular mass
Ribosomal DNA
Electrophoresis
Ethylenediaminetetraacetic acid
Sodium dodecyl sulfate
Edetic Acid
Sodium Dodecyl Sulfate
Acetic Acid
Polyacrylates
Acetic acid
Polyacrylamide Gel Electrophoresis
Sediments

Keywords

  • Deep-sea bacteria
  • Halophilic
  • Protease
  • Pseudoalteromonas

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

Cite this

Characterization of proteolytic bacteria from the Aleutian deep-sea and their proteases. / Xiong, Hairong; Song, Linsheng; Xu, Ying; Tsoi, Man Yee; Dobretsov, Sergey; Qian, Pei Yuan.

In: Journal of Industrial Microbiology and Biotechnology, Vol. 34, No. 1, 01.2007, p. 63-71.

Research output: Contribution to journalArticle

Xiong, Hairong ; Song, Linsheng ; Xu, Ying ; Tsoi, Man Yee ; Dobretsov, Sergey ; Qian, Pei Yuan. / Characterization of proteolytic bacteria from the Aleutian deep-sea and their proteases. In: Journal of Industrial Microbiology and Biotechnology. 2007 ; Vol. 34, No. 1. pp. 63-71.
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