Characterization of methylprednisolone esters of hyaluronan in aqueous solution: Conformation and aggregation behavior

Anna Taglienti, Massimiliano Valentini, Paolo Sequi, Vittorio Crescenzi

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Methylprednisolone steroid esters of hyaluronan differing in degree of functionalization and molecular weight were investigated in aqueous solution. Conformation and aggregation phenomena were elucidated by means of circular dichroism, viscometry, rheology, and nuclear magnetic resonance, mainly by 1H pulsed field gradient (PFG) NMR, which allows the determination of the diffusion coefficient of the species under investigation. The functionalization of hyaluronan with the steroid induces a reduction of the molecular volume, as a consequence of intramolecular hydrophobic interactions. For concentrated samples we have observed the coexistence of unimolecular collapsed chains and of aggregates, the latter disappearing upon dilution. The methylprednisolone ester of lower molecular weight hyaluronan has a larger molecular volume than its higher molecular weight analogue, even though still smaller than the underivatized polymer. This effect can be explained with the reduced flexibility of the polymer backbone probably impairing intramolecular interactions.

Original languageEnglish
Pages (from-to)1648-1653
Number of pages6
JournalBiomacromolecules
Volume6
Issue number3
DOIs
Publication statusPublished - May 2005

Fingerprint

Methylprednisolone
Hyaluronic Acid
Conformations
Esters
Agglomeration
Molecular weight
Polymers
Steroids
Nuclear magnetic resonance
Viscosity measurement
Dichroism
Rheology
Dilution

ASJC Scopus subject areas

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Characterization of methylprednisolone esters of hyaluronan in aqueous solution : Conformation and aggregation behavior. / Taglienti, Anna; Valentini, Massimiliano; Sequi, Paolo; Crescenzi, Vittorio.

In: Biomacromolecules, Vol. 6, No. 3, 05.2005, p. 1648-1653.

Research output: Contribution to journalArticle

@article{8aa1547492b84d3585adb375b5730c1a,
title = "Characterization of methylprednisolone esters of hyaluronan in aqueous solution: Conformation and aggregation behavior",
abstract = "Methylprednisolone steroid esters of hyaluronan differing in degree of functionalization and molecular weight were investigated in aqueous solution. Conformation and aggregation phenomena were elucidated by means of circular dichroism, viscometry, rheology, and nuclear magnetic resonance, mainly by 1H pulsed field gradient (PFG) NMR, which allows the determination of the diffusion coefficient of the species under investigation. The functionalization of hyaluronan with the steroid induces a reduction of the molecular volume, as a consequence of intramolecular hydrophobic interactions. For concentrated samples we have observed the coexistence of unimolecular collapsed chains and of aggregates, the latter disappearing upon dilution. The methylprednisolone ester of lower molecular weight hyaluronan has a larger molecular volume than its higher molecular weight analogue, even though still smaller than the underivatized polymer. This effect can be explained with the reduced flexibility of the polymer backbone probably impairing intramolecular interactions.",
author = "Anna Taglienti and Massimiliano Valentini and Paolo Sequi and Vittorio Crescenzi",
year = "2005",
month = "5",
doi = "10.1021/bm049221d",
language = "English",
volume = "6",
pages = "1648--1653",
journal = "Biomacromolecules",
issn = "1525-7797",
publisher = "American Chemical Society",
number = "3",

}

TY - JOUR

T1 - Characterization of methylprednisolone esters of hyaluronan in aqueous solution

T2 - Conformation and aggregation behavior

AU - Taglienti, Anna

AU - Valentini, Massimiliano

AU - Sequi, Paolo

AU - Crescenzi, Vittorio

PY - 2005/5

Y1 - 2005/5

N2 - Methylprednisolone steroid esters of hyaluronan differing in degree of functionalization and molecular weight were investigated in aqueous solution. Conformation and aggregation phenomena were elucidated by means of circular dichroism, viscometry, rheology, and nuclear magnetic resonance, mainly by 1H pulsed field gradient (PFG) NMR, which allows the determination of the diffusion coefficient of the species under investigation. The functionalization of hyaluronan with the steroid induces a reduction of the molecular volume, as a consequence of intramolecular hydrophobic interactions. For concentrated samples we have observed the coexistence of unimolecular collapsed chains and of aggregates, the latter disappearing upon dilution. The methylprednisolone ester of lower molecular weight hyaluronan has a larger molecular volume than its higher molecular weight analogue, even though still smaller than the underivatized polymer. This effect can be explained with the reduced flexibility of the polymer backbone probably impairing intramolecular interactions.

AB - Methylprednisolone steroid esters of hyaluronan differing in degree of functionalization and molecular weight were investigated in aqueous solution. Conformation and aggregation phenomena were elucidated by means of circular dichroism, viscometry, rheology, and nuclear magnetic resonance, mainly by 1H pulsed field gradient (PFG) NMR, which allows the determination of the diffusion coefficient of the species under investigation. The functionalization of hyaluronan with the steroid induces a reduction of the molecular volume, as a consequence of intramolecular hydrophobic interactions. For concentrated samples we have observed the coexistence of unimolecular collapsed chains and of aggregates, the latter disappearing upon dilution. The methylprednisolone ester of lower molecular weight hyaluronan has a larger molecular volume than its higher molecular weight analogue, even though still smaller than the underivatized polymer. This effect can be explained with the reduced flexibility of the polymer backbone probably impairing intramolecular interactions.

UR - http://www.scopus.com/inward/record.url?scp=20144372830&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=20144372830&partnerID=8YFLogxK

U2 - 10.1021/bm049221d

DO - 10.1021/bm049221d

M3 - Article

C2 - 15877390

AN - SCOPUS:20144372830

VL - 6

SP - 1648

EP - 1653

JO - Biomacromolecules

JF - Biomacromolecules

SN - 1525-7797

IS - 3

ER -