Characterization of hydrocarbon-degrading bacteria isolated from oil-contaminated sediments in the Sultanate of Oman and evaluation of bioaugmentation and biostimulation approaches in microcosm experiments

Raeid M M Abed, Jamal Al-Sabahi, Fatema Al-Maqrashi, Amal Al-Habsi, Manal Al-Hinai

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36 Citations (Scopus)

Abstract

Two oil-polluted sediments (PD and KH) were sampled from a coastal region in Oman for the isolation of hydrocarbon-degrading bacteria and for testing different bioremediation approaches. Fourty strains were isolated, eighteen were affiliated to Marinobacter whereas the rest belonged to Pseudomonas, Halomonas, Hahella and Alcanivorax. All strains grew well at 2-7% salinity and between 20 and 60°C. The strains exhibited a better growth on long chain than on short chain alkanes. Biostimulation and bioaugmentation were compared in both sediments and oil biodegradation was followed by measuring CO2 evolution and by gas chromatography (GC). The evolved CO2 reached 0.45±0.02 and 2.23±0.07mgCO2g-1 sediment after 88 days in the untreated PD and KH sediments, respectively. While the addition of inorganic nutrients resulted in 1.2-3.7 fold increase in CO2 evolution in both sediments, the addition of the bacterial consortium was only effective in the PD sediment. The maximum CO2 evolution was measured when both nutrients and bacteria were added and this corresponded to a total oil mineralization of 2.6±0.12 and 1.49±0.04% of the initial oil after 88 days in the PD and KH sediments, respectively. GC analysis confirmed the CO2 data and showed that most of the degraded compounds belonged to alkanes. We conclude that the Omani polluted sediments contain halotolerant and thermotolerant bacteria and biostimulation is more efficient than bioaugmentation for their cleanup.

Original languageEnglish
Pages (from-to)58-66
Number of pages9
JournalInternational Biodeterioration and Biodegradation
Volume89
DOIs
Publication statusPublished - Apr 2014

Fingerprint

Oman
Hydrocarbons
microcosm
Bacteria
Sediments
Oils
hydrocarbon
bacterium
Alkanes
oil
Gas Chromatography
sediment
Alcanivoraceae
Marinobacter
Halomonas
experiment
Experiments
Food
Environmental Biodegradation
Salinity

Keywords

  • 16S rRNA
  • Alkanes
  • Bacterial consortia
  • Bioaugmentation
  • Bioremediation
  • Nutrients

ASJC Scopus subject areas

  • Waste Management and Disposal
  • Microbiology
  • Biomaterials

Cite this

@article{40975f09865f48709fa5b6696788934e,
title = "Characterization of hydrocarbon-degrading bacteria isolated from oil-contaminated sediments in the Sultanate of Oman and evaluation of bioaugmentation and biostimulation approaches in microcosm experiments",
abstract = "Two oil-polluted sediments (PD and KH) were sampled from a coastal region in Oman for the isolation of hydrocarbon-degrading bacteria and for testing different bioremediation approaches. Fourty strains were isolated, eighteen were affiliated to Marinobacter whereas the rest belonged to Pseudomonas, Halomonas, Hahella and Alcanivorax. All strains grew well at 2-7{\%} salinity and between 20 and 60°C. The strains exhibited a better growth on long chain than on short chain alkanes. Biostimulation and bioaugmentation were compared in both sediments and oil biodegradation was followed by measuring CO2 evolution and by gas chromatography (GC). The evolved CO2 reached 0.45±0.02 and 2.23±0.07mgCO2g-1 sediment after 88 days in the untreated PD and KH sediments, respectively. While the addition of inorganic nutrients resulted in 1.2-3.7 fold increase in CO2 evolution in both sediments, the addition of the bacterial consortium was only effective in the PD sediment. The maximum CO2 evolution was measured when both nutrients and bacteria were added and this corresponded to a total oil mineralization of 2.6±0.12 and 1.49±0.04{\%} of the initial oil after 88 days in the PD and KH sediments, respectively. GC analysis confirmed the CO2 data and showed that most of the degraded compounds belonged to alkanes. We conclude that the Omani polluted sediments contain halotolerant and thermotolerant bacteria and biostimulation is more efficient than bioaugmentation for their cleanup.",
keywords = "16S rRNA, Alkanes, Bacterial consortia, Bioaugmentation, Bioremediation, Nutrients",
author = "Abed, {Raeid M M} and Jamal Al-Sabahi and Fatema Al-Maqrashi and Amal Al-Habsi and Manal Al-Hinai",
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T1 - Characterization of hydrocarbon-degrading bacteria isolated from oil-contaminated sediments in the Sultanate of Oman and evaluation of bioaugmentation and biostimulation approaches in microcosm experiments

AU - Abed, Raeid M M

AU - Al-Sabahi, Jamal

AU - Al-Maqrashi, Fatema

AU - Al-Habsi, Amal

AU - Al-Hinai, Manal

PY - 2014/4

Y1 - 2014/4

N2 - Two oil-polluted sediments (PD and KH) were sampled from a coastal region in Oman for the isolation of hydrocarbon-degrading bacteria and for testing different bioremediation approaches. Fourty strains were isolated, eighteen were affiliated to Marinobacter whereas the rest belonged to Pseudomonas, Halomonas, Hahella and Alcanivorax. All strains grew well at 2-7% salinity and between 20 and 60°C. The strains exhibited a better growth on long chain than on short chain alkanes. Biostimulation and bioaugmentation were compared in both sediments and oil biodegradation was followed by measuring CO2 evolution and by gas chromatography (GC). The evolved CO2 reached 0.45±0.02 and 2.23±0.07mgCO2g-1 sediment after 88 days in the untreated PD and KH sediments, respectively. While the addition of inorganic nutrients resulted in 1.2-3.7 fold increase in CO2 evolution in both sediments, the addition of the bacterial consortium was only effective in the PD sediment. The maximum CO2 evolution was measured when both nutrients and bacteria were added and this corresponded to a total oil mineralization of 2.6±0.12 and 1.49±0.04% of the initial oil after 88 days in the PD and KH sediments, respectively. GC analysis confirmed the CO2 data and showed that most of the degraded compounds belonged to alkanes. We conclude that the Omani polluted sediments contain halotolerant and thermotolerant bacteria and biostimulation is more efficient than bioaugmentation for their cleanup.

AB - Two oil-polluted sediments (PD and KH) were sampled from a coastal region in Oman for the isolation of hydrocarbon-degrading bacteria and for testing different bioremediation approaches. Fourty strains were isolated, eighteen were affiliated to Marinobacter whereas the rest belonged to Pseudomonas, Halomonas, Hahella and Alcanivorax. All strains grew well at 2-7% salinity and between 20 and 60°C. The strains exhibited a better growth on long chain than on short chain alkanes. Biostimulation and bioaugmentation were compared in both sediments and oil biodegradation was followed by measuring CO2 evolution and by gas chromatography (GC). The evolved CO2 reached 0.45±0.02 and 2.23±0.07mgCO2g-1 sediment after 88 days in the untreated PD and KH sediments, respectively. While the addition of inorganic nutrients resulted in 1.2-3.7 fold increase in CO2 evolution in both sediments, the addition of the bacterial consortium was only effective in the PD sediment. The maximum CO2 evolution was measured when both nutrients and bacteria were added and this corresponded to a total oil mineralization of 2.6±0.12 and 1.49±0.04% of the initial oil after 88 days in the PD and KH sediments, respectively. GC analysis confirmed the CO2 data and showed that most of the degraded compounds belonged to alkanes. We conclude that the Omani polluted sediments contain halotolerant and thermotolerant bacteria and biostimulation is more efficient than bioaugmentation for their cleanup.

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KW - Alkanes

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KW - Bioaugmentation

KW - Bioremediation

KW - Nutrients

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JF - International Biodeterioration and Biodegradation

SN - 0964-8305

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