Characterization of Carrageenan Extracted from Hypnea bryoides in Oman

Ahmed Ali Al-Alawi, Insaaf Mohammed Al-Marhubi, Mohammed Said Moosa Al-Belushi, Bassam Soussi

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Carrageenophyte red seaweed from Oman, Hypnea bryoides, extracted using three different processes: an aqueous, a mild alkaline, and a more vigorous alkaline extraction was investigated. The resulting extract precipitated by alcohol was subject to chemical and rheological measurements. The total carbohydrate [ranged from 36.78 to 41.65 g/100 g], and ash [39.04 to 43.11 g/100 g] were the most abundant components in H. bryoides and contrary to the two, lipid content was found at a minimum [ranging from 2.95 to 3.38 g/100 g]. Alkali treatment with NaOH allowed complete conversion of kappa (κ) carrageenan form as detected by FTIR analysis. Total yield by alkali treatments gave higher yields (33%) compared with aqueous treatments (12%). However, subsequent aqueous treatment produced mixed carrageenan (μ and κ) with higher molecular weight compared with the alkali treatments which produced single carrageenan form (κ) with molecular weight of 4.1 × 10 5 Da. The effects of thermal history on gel-sol and sol-gel transition were investigated by differential scanning calorimeter (DSC) and rheology on a pure sample and 1.5% κ-carrageenan mixture added with 30 mM KCl. Transition temperatures from DSC and rheology showed comparable results and were in good agreement with those previously reported.

Original languageEnglish
Pages (from-to)893-899
Number of pages7
JournalMarine Biotechnology
Volume13
Issue number5
DOIs
Publication statusPublished - Oct 2011

Fingerprint

Hypnea
Oman
carrageenan
alkali treatment
Carrageenan
Alkalies
calorimeters
rheology
water treatment
Rheology
Polymethyl Methacrylate
gels
molecular weight
kappa carrageenan
gel
Molecular Weight
Gels
Seaweed
macroalgae
Transition Temperature

Keywords

  • Algae
  • FTIR
  • Hypnea bryoides
  • Phycocolloids
  • Seaweed

ASJC Scopus subject areas

  • Biotechnology
  • Aquatic Science

Cite this

Characterization of Carrageenan Extracted from Hypnea bryoides in Oman. / Al-Alawi, Ahmed Ali; Al-Marhubi, Insaaf Mohammed; Al-Belushi, Mohammed Said Moosa; Soussi, Bassam.

In: Marine Biotechnology, Vol. 13, No. 5, 10.2011, p. 893-899.

Research output: Contribution to journalArticle

Al-Alawi, Ahmed Ali ; Al-Marhubi, Insaaf Mohammed ; Al-Belushi, Mohammed Said Moosa ; Soussi, Bassam. / Characterization of Carrageenan Extracted from Hypnea bryoides in Oman. In: Marine Biotechnology. 2011 ; Vol. 13, No. 5. pp. 893-899.
@article{d03b590a557442ad9ac3c4f17bcb6d01,
title = "Characterization of Carrageenan Extracted from Hypnea bryoides in Oman",
abstract = "Carrageenophyte red seaweed from Oman, Hypnea bryoides, extracted using three different processes: an aqueous, a mild alkaline, and a more vigorous alkaline extraction was investigated. The resulting extract precipitated by alcohol was subject to chemical and rheological measurements. The total carbohydrate [ranged from 36.78 to 41.65 g/100 g], and ash [39.04 to 43.11 g/100 g] were the most abundant components in H. bryoides and contrary to the two, lipid content was found at a minimum [ranging from 2.95 to 3.38 g/100 g]. Alkali treatment with NaOH allowed complete conversion of kappa (κ) carrageenan form as detected by FTIR analysis. Total yield by alkali treatments gave higher yields (33{\%}) compared with aqueous treatments (12{\%}). However, subsequent aqueous treatment produced mixed carrageenan (μ and κ) with higher molecular weight compared with the alkali treatments which produced single carrageenan form (κ) with molecular weight of 4.1 × 10 5 Da. The effects of thermal history on gel-sol and sol-gel transition were investigated by differential scanning calorimeter (DSC) and rheology on a pure sample and 1.5{\%} κ-carrageenan mixture added with 30 mM KCl. Transition temperatures from DSC and rheology showed comparable results and were in good agreement with those previously reported.",
keywords = "Algae, FTIR, Hypnea bryoides, Phycocolloids, Seaweed",
author = "Al-Alawi, {Ahmed Ali} and Al-Marhubi, {Insaaf Mohammed} and Al-Belushi, {Mohammed Said Moosa} and Bassam Soussi",
year = "2011",
month = "10",
doi = "10.1007/s10126-010-9350-7",
language = "English",
volume = "13",
pages = "893--899",
journal = "Marine Biotechnology",
issn = "1436-2228",
publisher = "Springer New York",
number = "5",

}

TY - JOUR

T1 - Characterization of Carrageenan Extracted from Hypnea bryoides in Oman

AU - Al-Alawi, Ahmed Ali

AU - Al-Marhubi, Insaaf Mohammed

AU - Al-Belushi, Mohammed Said Moosa

AU - Soussi, Bassam

PY - 2011/10

Y1 - 2011/10

N2 - Carrageenophyte red seaweed from Oman, Hypnea bryoides, extracted using three different processes: an aqueous, a mild alkaline, and a more vigorous alkaline extraction was investigated. The resulting extract precipitated by alcohol was subject to chemical and rheological measurements. The total carbohydrate [ranged from 36.78 to 41.65 g/100 g], and ash [39.04 to 43.11 g/100 g] were the most abundant components in H. bryoides and contrary to the two, lipid content was found at a minimum [ranging from 2.95 to 3.38 g/100 g]. Alkali treatment with NaOH allowed complete conversion of kappa (κ) carrageenan form as detected by FTIR analysis. Total yield by alkali treatments gave higher yields (33%) compared with aqueous treatments (12%). However, subsequent aqueous treatment produced mixed carrageenan (μ and κ) with higher molecular weight compared with the alkali treatments which produced single carrageenan form (κ) with molecular weight of 4.1 × 10 5 Da. The effects of thermal history on gel-sol and sol-gel transition were investigated by differential scanning calorimeter (DSC) and rheology on a pure sample and 1.5% κ-carrageenan mixture added with 30 mM KCl. Transition temperatures from DSC and rheology showed comparable results and were in good agreement with those previously reported.

AB - Carrageenophyte red seaweed from Oman, Hypnea bryoides, extracted using three different processes: an aqueous, a mild alkaline, and a more vigorous alkaline extraction was investigated. The resulting extract precipitated by alcohol was subject to chemical and rheological measurements. The total carbohydrate [ranged from 36.78 to 41.65 g/100 g], and ash [39.04 to 43.11 g/100 g] were the most abundant components in H. bryoides and contrary to the two, lipid content was found at a minimum [ranging from 2.95 to 3.38 g/100 g]. Alkali treatment with NaOH allowed complete conversion of kappa (κ) carrageenan form as detected by FTIR analysis. Total yield by alkali treatments gave higher yields (33%) compared with aqueous treatments (12%). However, subsequent aqueous treatment produced mixed carrageenan (μ and κ) with higher molecular weight compared with the alkali treatments which produced single carrageenan form (κ) with molecular weight of 4.1 × 10 5 Da. The effects of thermal history on gel-sol and sol-gel transition were investigated by differential scanning calorimeter (DSC) and rheology on a pure sample and 1.5% κ-carrageenan mixture added with 30 mM KCl. Transition temperatures from DSC and rheology showed comparable results and were in good agreement with those previously reported.

KW - Algae

KW - FTIR

KW - Hypnea bryoides

KW - Phycocolloids

KW - Seaweed

UR - http://www.scopus.com/inward/record.url?scp=80051671251&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80051671251&partnerID=8YFLogxK

U2 - 10.1007/s10126-010-9350-7

DO - 10.1007/s10126-010-9350-7

M3 - Article

C2 - 21240534

AN - SCOPUS:80051671251

VL - 13

SP - 893

EP - 899

JO - Marine Biotechnology

JF - Marine Biotechnology

SN - 1436-2228

IS - 5

ER -