Characterization of calcium signals provoked by lysophosphatidylinositol in human microvascular endothelial cells

Y. M. Al Suleimani, C. R. Hiley

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The lipid molecule, lysophosphatidylinositol (LPI), is hypothesised to form part of a novel lipid signalling system that involves the G protein-coupled receptor GPR55 and distinct intracellular signalling cascades in endothelial cells. This work aimed to study the possible mechanisms involved in LPI-evoked cytosolic Ca2+ mobilization in human brain microvascular endothelial cells. Changes in intracellular Ca2+ concentrations were measured using cell population Ca2+ assay. LPI evoked biphasic elevation of intracellular calcium concentration, a rapid phase and a sustained phase. The rapid phase was attenuated by the inhibitor of PLC (U 73122), inhibitor of IP3 receptors, 2-APB and the depletor of endoplasmic reticulum Ca2+ store, thapsigargin. The sustained phase, on the other hand, was enhanced by U 73122 and abolished by the RhoA kinase inhibitor, Y-27632. In conclusion, the Ca2+ signal evoked by LPI is characterised by a rapid phase of Ca2+ release from the endoplasmic reticulum, and requires activation of the PLC-IP3 signalling pathway. The sustained phase mainly depends on RhoA kinase activation. LPI acts as novel lipid signalling molecule in endothelial cells, and elevation of cytosolic Ca2+ triggered by it may present an important intracellular message required in gene expression and controlling of vascular tone.

Original languageEnglish
Pages (from-to)53-62
Number of pages10
JournalPhysiological Research
Volume65
Issue number1
Publication statusPublished - 2016

Keywords

  • Calcium
  • GPR55
  • Lysophosphatidylinositol
  • PLC
  • RhoA kinase

ASJC Scopus subject areas

  • Physiology

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