Characterization of calcium signals provoked by lysophosphatidylinositol in human microvascular endothelial cells

Y. M. Al Suleimani, C. R. Hiley

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The lipid molecule, lysophosphatidylinositol (LPI), is hypothesised to form part of a novel lipid signalling system that involves the G protein-coupled receptor GPR55 and distinct intracellular signalling cascades in endothelial cells. This work aimed to study the possible mechanisms involved in LPI-evoked cytosolic Ca2+ mobilization in human brain microvascular endothelial cells. Changes in intracellular Ca2+ concentrations were measured using cell population Ca2+ assay. LPI evoked biphasic elevation of intracellular calcium concentration, a rapid phase and a sustained phase. The rapid phase was attenuated by the inhibitor of PLC (U 73122), inhibitor of IP3 receptors, 2-APB and the depletor of endoplasmic reticulum Ca2+ store, thapsigargin. The sustained phase, on the other hand, was enhanced by U 73122 and abolished by the RhoA kinase inhibitor, Y-27632. In conclusion, the Ca2+ signal evoked by LPI is characterised by a rapid phase of Ca2+ release from the endoplasmic reticulum, and requires activation of the PLC-IP3 signalling pathway. The sustained phase mainly depends on RhoA kinase activation. LPI acts as novel lipid signalling molecule in endothelial cells, and elevation of cytosolic Ca2+ triggered by it may present an important intracellular message required in gene expression and controlling of vascular tone.

Original languageEnglish
Pages (from-to)53-62
Number of pages10
JournalPhysiological Research
Volume65
Issue number1
Publication statusPublished - 2016

Fingerprint

Endothelial Cells
Calcium
Lipids
Endoplasmic Reticulum
Phosphotransferases
Inositol 1,4,5-Trisphosphate Receptors
Thapsigargin
G-Protein-Coupled Receptors
Blood Vessels
lysophosphatidylinositol
Gene Expression
Brain
Population
1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione

Keywords

  • Calcium
  • GPR55
  • Lysophosphatidylinositol
  • PLC
  • RhoA kinase

ASJC Scopus subject areas

  • Physiology

Cite this

Characterization of calcium signals provoked by lysophosphatidylinositol in human microvascular endothelial cells. / Al Suleimani, Y. M.; Hiley, C. R.

In: Physiological Research, Vol. 65, No. 1, 2016, p. 53-62.

Research output: Contribution to journalArticle

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