Characterization of antimicrobial peptides from the skin secretions of the Malaysian frogs, Odorrana hosii and Hylarana picturata (Anura: Ranidae)

J. Michael Conlon, Jolanta Kolodziejek, Norbert Nowotny, Jérôme Leprince, Hubert Vaudry, Laurent Coquet, Thierry Jouenne, Jay D. King

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Peptidomic analysis of norepinephrine-stimulated skin secretions from Hose's rock frog Odorrana hosii (Boulenger, 1891) led to the isolation of 8 peptides with differential antibacterial activities. Structural characterization demonstrated that the peptides belonged to the esculentin-1 (1 peptide), esculentin-2 (1 peptide), brevinin-1 (2 peptides), brevinin-2 (2 peptides), and nigrocin-2 (2 peptides) families of antimicrobial peptides. Similar analysis of skin secretions from the Malaysian fire frog Hylarana picturata (Boulenger, 1920) led to the isolation and characterization of peptides belonging to the brevinin-1 (2 peptides), brevinin-2 (5 peptides), and temporin (1 peptide) families. The differences in antimicrobial activities of paralogous peptides provide insight into structure-activity relationships, emphasizing the importance of cationicity in determining potency. The substitution Lys11 → Gln in brevinin-1HSa (FLPAVLRVAAKIVPTVFCAISKKC) from O. hosii abolishes growth inhibitory activity against Escherichia coli but has no effect on the high potency (MIC = 8 μg/ml) against Staphylococcus aureus. In contrast, the substitution (Gly4 → Asp) in brevinin-2PTb (GFKGAFKNVMFGIAKSAGKSALNALACKIDKSC) from H. picturata reduces activity against both E. coli and S. aureus. Cladistic analysis based upon the amino acid sequences of the brevinin-2 peptides from Asian frogs provides evidence for sister taxon relationships between O. hosii and O. livida and between H. picturata and H. güntheri.

Original languageEnglish
Pages (from-to)465-473
Number of pages9
JournalToxicon
Volume52
Issue number3
DOIs
Publication statusPublished - Sep 1 2008

Fingerprint

Ranidae
Anura
Skin
Peptides
Escherichia coli
Staphylococcus aureus
Substitution reactions
Hose
Structure-Activity Relationship
Viperidae

Keywords

  • Antimicrobial peptide
  • Brevinin
  • Esculentin
  • Frog skin
  • Nigrocin
  • Phylogeny

ASJC Scopus subject areas

  • Toxicology

Cite this

Characterization of antimicrobial peptides from the skin secretions of the Malaysian frogs, Odorrana hosii and Hylarana picturata (Anura : Ranidae). / Conlon, J. Michael; Kolodziejek, Jolanta; Nowotny, Norbert; Leprince, Jérôme; Vaudry, Hubert; Coquet, Laurent; Jouenne, Thierry; King, Jay D.

In: Toxicon, Vol. 52, No. 3, 01.09.2008, p. 465-473.

Research output: Contribution to journalArticle

Conlon, J. Michael ; Kolodziejek, Jolanta ; Nowotny, Norbert ; Leprince, Jérôme ; Vaudry, Hubert ; Coquet, Laurent ; Jouenne, Thierry ; King, Jay D. / Characterization of antimicrobial peptides from the skin secretions of the Malaysian frogs, Odorrana hosii and Hylarana picturata (Anura : Ranidae). In: Toxicon. 2008 ; Vol. 52, No. 3. pp. 465-473.
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