Characterization and Application of Biosurfactant Produced by Bacillus licheniformis R2

Sanket J. Joshi, S. J. Geetha, Anjana J. Desai

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The biosurfactant produced by Bacillus licheniformis R2 was characterized and studied for enhancing the heavy crude oil recovery at 80 °C in coreflood experiments. The strain was found to be nonpathogenic and produced biosurfactant, reducing the surface tension of medium from 70 to 28 mN/m with 1.1 g/l yield. The biosurfactant was quite stable during exposure to elevated temperatures (85 °C for 90 days), high salinity (10 % NaCl), and a wide range of pH (5–12) for 10 days. It was characterized as lipopeptide similar to lichenysin-A, with a critical micelle concentration of about 19.4 mg/l. The efficiency of crude biosurfactant for enhanced oil recovery by core flood studies revealed it to recovering additional 37.1 % oil from Berea sandstone cores at 80 °C. The results are indicative of the potential for the development of lipopeptide biosurfactant-based ex situ microbial enhanced heavy oil recovery from depleting oil fields with extreme temperatures.

Original languageEnglish
Pages (from-to)346-361
Number of pages16
JournalApplied Biochemistry and Biotechnology
Volume177
Issue number2
DOIs
Publication statusPublished - Jul 18 2015

Keywords

  • Bacillus licheniformis
  • Biosurfactant
  • Lichenysin
  • Microbial enhanced oil recovery

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Biology

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