Characterization and Application of Biosurfactant Produced by Bacillus licheniformis R2

Sanket J. Joshi*, S. J. Geetha, Anjana J. Desai

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

50 Citations (SciVal)


The biosurfactant produced by Bacillus licheniformis R2 was characterized and studied for enhancing the heavy crude oil recovery at 80 °C in coreflood experiments. The strain was found to be nonpathogenic and produced biosurfactant, reducing the surface tension of medium from 70 to 28 mN/m with 1.1 g/l yield. The biosurfactant was quite stable during exposure to elevated temperatures (85 °C for 90 days), high salinity (10 % NaCl), and a wide range of pH (5–12) for 10 days. It was characterized as lipopeptide similar to lichenysin-A, with a critical micelle concentration of about 19.4 mg/l. The efficiency of crude biosurfactant for enhanced oil recovery by core flood studies revealed it to recovering additional 37.1 % oil from Berea sandstone cores at 80 °C. The results are indicative of the potential for the development of lipopeptide biosurfactant-based ex situ microbial enhanced heavy oil recovery from depleting oil fields with extreme temperatures.

Original languageEnglish
Pages (from-to)346-361
Number of pages16
JournalApplied Biochemistry and Biotechnology
Issue number2
Publication statusPublished - Sept 28 2015


  • Bacillus licheniformis
  • Biosurfactant
  • Lichenysin
  • Microbial enhanced oil recovery

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology


Dive into the research topics of 'Characterization and Application of Biosurfactant Produced by Bacillus licheniformis R2'. Together they form a unique fingerprint.

Cite this