Characterization and Application of Biosurfactant Produced by Bacillus licheniformis R2

Sanket J. Joshi, S. J. Geetha, Anjana J. Desai

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The biosurfactant produced by Bacillus licheniformis R2 was characterized and studied for enhancing the heavy crude oil recovery at 80 °C in coreflood experiments. The strain was found to be nonpathogenic and produced biosurfactant, reducing the surface tension of medium from 70 to 28 mN/m with 1.1 g/l yield. The biosurfactant was quite stable during exposure to elevated temperatures (85 °C for 90 days), high salinity (10 % NaCl), and a wide range of pH (5–12) for 10 days. It was characterized as lipopeptide similar to lichenysin-A, with a critical micelle concentration of about 19.4 mg/l. The efficiency of crude biosurfactant for enhanced oil recovery by core flood studies revealed it to recovering additional 37.1 % oil from Berea sandstone cores at 80 °C. The results are indicative of the potential for the development of lipopeptide biosurfactant-based ex situ microbial enhanced heavy oil recovery from depleting oil fields with extreme temperatures.

Original languageEnglish
Pages (from-to)346-361
Number of pages16
JournalApplied Biochemistry and Biotechnology
Volume177
Issue number2
DOIs
Publication statusPublished - Jul 18 2015

Fingerprint

Bacilli
Lipopeptides
Oils
Recovery
Crude oil
Oil and Gas Fields
Temperature
Surface Tension
Critical micelle concentration
Salinity
Petroleum
Micelles
Oil fields
Sandstone
Surface tension
Bacillus licheniformis
Experiments

Keywords

  • Bacillus licheniformis
  • Biosurfactant
  • Lichenysin
  • Microbial enhanced oil recovery

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Biology

Cite this

Characterization and Application of Biosurfactant Produced by Bacillus licheniformis R2. / Joshi, Sanket J.; Geetha, S. J.; Desai, Anjana J.

In: Applied Biochemistry and Biotechnology, Vol. 177, No. 2, 18.07.2015, p. 346-361.

Research output: Contribution to journalArticle

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