Changes in bacillus thuringiensis tolerance levels due to hybridization of Bt-tolerant and susceptible silkworm populations

Hosne Ara Begum, Errol Hassan, John Dingle, Aisha Alkhayat Alshehi

Research output: Contribution to journalArticle

Abstract

Males and females of a Bt-tolerant mulberry silkworm (Bombyx mori L.) population were crossed with females and males of a Bt-susceptible population, to produce Bt-tolerant silkworm hybrids, and to determine the expression of the Bt-tolerance pattern in the Fl hybrids. It was observed that when a Bt-tolerant (42% larval mortality) female (BtT ♀) silkworm was crossed with a Bt-susceptible (85% larval mortality) male (BtS ♂, the resultant Fl offspring showed lower levels of Bt-tolerance (87% larval mortality). On the other hand, when a Bt-tolerant male (BtT ♂ was crossed with a Bt-susceptible female (BtS ♀, the F1 hybrid showed higher levels of Bt-tolerance (35% larval mortality) characteristic. The probit statistics showed that both hybrids expressed Bt-tolerance or susceptible levels similar to their male parents. These different patterns of Bt-tolerance in Fl hybrids might be due to the transferring of a Bttolerant gene, from the parents to offspring, through the homozygotic male (ZZ) silkworm.

Original languageEnglish
Pages (from-to)101-107
Number of pages7
JournalPakistan Journal of Scientific and Industrial Research Series B: Biological Sciences
Volume55
Issue number2
Publication statusPublished - Jul 2012

Fingerprint

Bacillus thuringiensis
Bombyx
silkworms
hybridization
Population
Mortality
Morus
mulberries
at-risk population
Bombyx mori
statistics
genes
Genes

Keywords

  • Bacillus thuringiensis
  • Bombyx mori
  • Bt-tolerance
  • Hybrid performances
  • Insect hybridization
  • Male homozygosity

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

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abstract = "Males and females of a Bt-tolerant mulberry silkworm (Bombyx mori L.) population were crossed with females and males of a Bt-susceptible population, to produce Bt-tolerant silkworm hybrids, and to determine the expression of the Bt-tolerance pattern in the Fl hybrids. It was observed that when a Bt-tolerant (42{\%} larval mortality) female (BtT ♀) silkworm was crossed with a Bt-susceptible (85{\%} larval mortality) male (BtS ♂, the resultant Fl offspring showed lower levels of Bt-tolerance (87{\%} larval mortality). On the other hand, when a Bt-tolerant male (BtT ♂ was crossed with a Bt-susceptible female (BtS ♀, the F1 hybrid showed higher levels of Bt-tolerance (35{\%} larval mortality) characteristic. The probit statistics showed that both hybrids expressed Bt-tolerance or susceptible levels similar to their male parents. These different patterns of Bt-tolerance in Fl hybrids might be due to the transferring of a Bttolerant gene, from the parents to offspring, through the homozygotic male (ZZ) silkworm.",
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T1 - Changes in bacillus thuringiensis tolerance levels due to hybridization of Bt-tolerant and susceptible silkworm populations

AU - Begum, Hosne Ara

AU - Hassan, Errol

AU - Dingle, John

AU - Alshehi, Aisha Alkhayat

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AB - Males and females of a Bt-tolerant mulberry silkworm (Bombyx mori L.) population were crossed with females and males of a Bt-susceptible population, to produce Bt-tolerant silkworm hybrids, and to determine the expression of the Bt-tolerance pattern in the Fl hybrids. It was observed that when a Bt-tolerant (42% larval mortality) female (BtT ♀) silkworm was crossed with a Bt-susceptible (85% larval mortality) male (BtS ♂, the resultant Fl offspring showed lower levels of Bt-tolerance (87% larval mortality). On the other hand, when a Bt-tolerant male (BtT ♂ was crossed with a Bt-susceptible female (BtS ♀, the F1 hybrid showed higher levels of Bt-tolerance (35% larval mortality) characteristic. The probit statistics showed that both hybrids expressed Bt-tolerance or susceptible levels similar to their male parents. These different patterns of Bt-tolerance in Fl hybrids might be due to the transferring of a Bttolerant gene, from the parents to offspring, through the homozygotic male (ZZ) silkworm.

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