Cardiac myocyte-secreted cAMP exerts paracrine action via adenosine receptor activation

Yassine Sassi, Andrea Ahles, Dong Jiunn Jeffery Truong, Younis Baqi, Sang Yong Lee, Britta Husse, Jean Sébastien Hulot, Ariana Foinquinos, Thomas Thum, Christa E. Müller, Andreas Dendorfer, Bernhard Laggerbauer, Stefan Engelhardt

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Acute stimulation of cardiac β-adrenoceptors is crucial to increasing cardiac function under stress; however, sustained β-adrenergic stimulation has been implicated in pathological myocardial remodeling and heart failure. Here, we have demonstrated that export of cAMP from cardiac myocytes is an intrinsic cardioprotective mechanism in response to cardiac stress. We report that infusion of cAMP into mice averted myocardial hypertrophy and fibrosis in a disease model of cardiac pressure overload. The protective effect of exogenous cAMP required adenosine receptor signaling. This observation led to the identification of a potent paracrine mechanism that is dependent on secreted cAMP. Specifically, FRET-based imaging of cAMP formation in primary cells and in myocardial tissue from murine hearts revealed that cardiomyocytes depend on the transporter ABCC4 to export cAMP as an extracellular signal. Extracellular cAMP, through its metabolite adenosine, reduced cardiomyocyte cAMP formation and hypertrophy by activating A1 adenosine receptors while delivering an antifibrotic signal to cardiac fibroblasts by A2 adenosine receptor activation. Together, our data reveal a paracrine role for secreted cAMP in intercellular signaling in the myocardium, and we postulate that secreted cAMP may also constitute an important signal in other tissues.

Original languageEnglish
Pages (from-to)5385-5397
Number of pages13
JournalJournal of Clinical Investigation
Volume124
Issue number12
DOIs
Publication statusPublished - Dec 1 2014

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Purinergic P1 Receptors
Cardiac Myocytes
Hypertrophy
Heart Failure
Adenosine A2 Receptors
Adenosine A1 Receptors
Adrenergic Agents
Adenosine
Adrenergic Receptors
Heart Diseases
Myocardium
Fibrosis
Fibroblasts
Pressure

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Sassi, Y., Ahles, A., Truong, D. J. J., Baqi, Y., Lee, S. Y., Husse, B., ... Engelhardt, S. (2014). Cardiac myocyte-secreted cAMP exerts paracrine action via adenosine receptor activation. Journal of Clinical Investigation, 124(12), 5385-5397. https://doi.org/10.1172/JCI74349

Cardiac myocyte-secreted cAMP exerts paracrine action via adenosine receptor activation. / Sassi, Yassine; Ahles, Andrea; Truong, Dong Jiunn Jeffery; Baqi, Younis; Lee, Sang Yong; Husse, Britta; Hulot, Jean Sébastien; Foinquinos, Ariana; Thum, Thomas; Müller, Christa E.; Dendorfer, Andreas; Laggerbauer, Bernhard; Engelhardt, Stefan.

In: Journal of Clinical Investigation, Vol. 124, No. 12, 01.12.2014, p. 5385-5397.

Research output: Contribution to journalArticle

Sassi, Y, Ahles, A, Truong, DJJ, Baqi, Y, Lee, SY, Husse, B, Hulot, JS, Foinquinos, A, Thum, T, Müller, CE, Dendorfer, A, Laggerbauer, B & Engelhardt, S 2014, 'Cardiac myocyte-secreted cAMP exerts paracrine action via adenosine receptor activation', Journal of Clinical Investigation, vol. 124, no. 12, pp. 5385-5397. https://doi.org/10.1172/JCI74349
Sassi, Yassine ; Ahles, Andrea ; Truong, Dong Jiunn Jeffery ; Baqi, Younis ; Lee, Sang Yong ; Husse, Britta ; Hulot, Jean Sébastien ; Foinquinos, Ariana ; Thum, Thomas ; Müller, Christa E. ; Dendorfer, Andreas ; Laggerbauer, Bernhard ; Engelhardt, Stefan. / Cardiac myocyte-secreted cAMP exerts paracrine action via adenosine receptor activation. In: Journal of Clinical Investigation. 2014 ; Vol. 124, No. 12. pp. 5385-5397.
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