'Candidatus Phytoplasma omanense', associated with witches'-broom of Cassia italica (Mill.) Spreng. in Oman

Nadiya Abubakar Al-Saady, Akhtar Jamal Khan, Alberto Calari, Ali Masoud Al-Subhi, Assunta Bertaccini

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Samples from plants of Cassia italica exhibiting typical witches'-broom symptoms (Cassia witches'-broom; CWB) were examined for the presence of plant pathogenic phytoplasmas by PCR amplification using universal phytoplasma primers. All affected plants yielded positive results. RFLP analyses of rRNA gene products indicated that the phytoplasmas detected were different from those described previously. Phylogenetic analysis of 16S rRNA gene sequences confirmed that CWB represents a distinct lineage and shares a common ancestor with 'Candidatus Phytoplasma phoenicium'. Molecular comparison revealed that the 16S rRNA gene sequences of the four CWB strains (IM-1, IM-2, IM-3 and IM-4) identified in symptomatic C. italica samples were nearly identical (99.6-100 % similarity). The closest relatives were members of the pigeon pea witches'-broom phytoplasma ribosomal group (16SrIX; 95-97 % sequence similarity). On the basis of unique 16S rRNA gene sequences and biological properties, the phytoplasma associated with witches'-broom of C. italica in Oman represents a coherent but discrete novel phytoplasma, 'Candidatus Phytoplasma omanense', with GenBank/DDBJ/EMBL accession number EF666051 representing the reference strain.

Original languageEnglish
Pages (from-to)461-466
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume58
Issue number2
DOIs
Publication statusPublished - Feb 2008

Fingerprint

Phytoplasma
phytoplasma
Cassia
Oman
witches' broom
rRNA Genes
ribosomal RNA
gene
nucleotide sequences
Nucleic Acid Databases
Pigeon pea witches'-broom phytoplasma
16SrIX (Pigeon pea witches'-broom group)
Peas
Columbidae
common ancestry
signs and symptoms (plants)
Restriction Fragment Length Polymorphisms
restriction fragment length polymorphism
ancestry
amplification

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Applied Microbiology and Biotechnology
  • Immunology
  • Microbiology

Cite this

'Candidatus Phytoplasma omanense', associated with witches'-broom of Cassia italica (Mill.) Spreng. in Oman. / Al-Saady, Nadiya Abubakar; Khan, Akhtar Jamal; Calari, Alberto; Al-Subhi, Ali Masoud; Bertaccini, Assunta.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 58, No. 2, 02.2008, p. 461-466.

Research output: Contribution to journalArticle

Al-Saady, Nadiya Abubakar ; Khan, Akhtar Jamal ; Calari, Alberto ; Al-Subhi, Ali Masoud ; Bertaccini, Assunta. / 'Candidatus Phytoplasma omanense', associated with witches'-broom of Cassia italica (Mill.) Spreng. in Oman. In: International Journal of Systematic and Evolutionary Microbiology. 2008 ; Vol. 58, No. 2. pp. 461-466.
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