Burkholderia sp. strain TNAU-1 for biological control of root rot in mung bean (Vigna radiata L.) caused by Macrophomina phaseolina

Vijayalakshmi Satya, Ayyathurai Vijayasamundeeswari, Vaikuntavasan Paranidharan, Rethinasamy Velazhahan

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The potential of Burkholderia sp. strain TNAU-1 for the management of mung bean (Vigna radiata L.) root rot caused by Macrophomina phaseolina was evaluated under greenhouse conditions. Burkholderia sp. strain TNAU-1 inhibited the mycelial growth of M. phaseolina in vitro and produced an inhibition zone of 18.8 mm. Mung bean seeds when treated with the bacterial suspension, showed significant increase in root length, shoot length and seedling vigour. A talc-based powder formulation of Burkholderia sp. strain TNAU-1 was developed and evaluated for its efficacy in the management of mung bean root rot under greenhouse conditions. Seed treatment or soil application of the powder formulation of Burkholderia sp. strain TNAU-1 significantly reduced the incidence of root rot and increased the germination percentage and plant height. Seed treatment with the powder formulation of Burkholderia sp. strain TNAU-1 alone was effective in controlling root rot disease; but the combined seed treatment and soil application of Burkholderia sp. strain TNAU-1, increased the efficacy. Seed treatment and soil application with Burkholderia sp. reduced the root rot incidence from 52.6 per cent (with non-bacterized seeds) to 16.7 per cent. Control of root rot with the application of Burkholderia sp. by seed treatment and soil application was not statistically different from that obtained with seed treatment with carbendazim. The endophytic movement of Burkholderia sp. in the stem, roots and leaves of mung bean was confirmed through PCR using Burkholderia sp. specific primers which resulted in the amplification of a 417 bp product.

Original languageEnglish
Pages (from-to)273-278
Number of pages6
JournalJournal of Plant Protection Research
Volume51
Issue number3
DOIs
Publication statusPublished - Jul 1 2011

Fingerprint

seed treatment
root rot
Burkholderia
Macrophomina phaseolina
Vigna radiata
mung beans
biological control
soil treatment
soil
powders
carbendazim
seed
talc
vigor
amplification
germination
greenhouses
incidence
shoot
stem

Keywords

  • biocontrol
  • Burkholderia sp.
  • Macrophomina phaseolina
  • mung bean

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Soil Science
  • Plant Science

Cite this

Burkholderia sp. strain TNAU-1 for biological control of root rot in mung bean (Vigna radiata L.) caused by Macrophomina phaseolina. / Satya, Vijayalakshmi; Vijayasamundeeswari, Ayyathurai; Paranidharan, Vaikuntavasan; Velazhahan, Rethinasamy.

In: Journal of Plant Protection Research, Vol. 51, No. 3, 01.07.2011, p. 273-278.

Research output: Contribution to journalArticle

Satya, Vijayalakshmi ; Vijayasamundeeswari, Ayyathurai ; Paranidharan, Vaikuntavasan ; Velazhahan, Rethinasamy. / Burkholderia sp. strain TNAU-1 for biological control of root rot in mung bean (Vigna radiata L.) caused by Macrophomina phaseolina. In: Journal of Plant Protection Research. 2011 ; Vol. 51, No. 3. pp. 273-278.
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