TY - JOUR
T1 - Bioengineered chitosan-iron nanocomposite controls bacterial leaf blight disease by modulating plant defense response and nutritional status of rice (Oryza sativa L.)
AU - Ahmed, Temoor
AU - Noman, Muhammad
AU - Jiang, Hubiao
AU - Shahid, Muhammad
AU - Ma, Chuanxin
AU - Wu, Zhifeng
AU - Nazir, Muhammad Mudassir
AU - Ali, Md Arshad
AU - White, Jason C.
AU - Chen, Jianping
AU - Li, Bin
N1 - Funding Information:
This work is financially supported by National Natural Science Foundation of China ( 31872017 , 32072472 ), Zhejiang Provincial Natural Science Foundation of China ( LZ19C140002 ), Shanghai Agriculture Applied Technology Development Program ( 2021-02-08-00-12-F00771 ), Zhejiang Provincial Project ( 2019C02006 , 2020C02006 ), State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products (grant number 2010DS700124-ZZ2014;-KF202101 ).
Publisher Copyright:
© 2022 Elsevier Ltd
PY - 2022/8
Y1 - 2022/8
N2 - Rice production is severely affected by the bacterial pathogen, Xanthomonas oryzae pv. oryzae (Xoo), which causes bacterial leaf blight (BLB) disease. Nano-enabled strategies have recently attracted attention as a sustainable platform to improve crop nutrition and protection. Here, we report the bioengineering of chitosan-iron nanocomposites (BNCs) along with their in vitro and in vivo bactericidal activity against Xoo. In addition, the impact of BNCs on the endophytic microbiome of healthy and BLB diseased rice was evaluated using a high-throughput sequencing technique. The BNCs were spherically shaped with an average size of 86 nm. In vitro antibacterial assays showed that BNCs significantly inhibited biological functions of the pathogen (viz., growth, motility, and biofilm formation) at 250 μg mL−1 concentration as compared with respective control. A greenhouse experiment demonstrated that foliar exposure to 250 mg·L−1 BNCs significantly reduced the BLB disease incidence (67.1%) through modulation of antioxidant enzymes viz., superoxide dismutase (49.2%), peroxidase (38.8%) and ascorbate peroxidase (53.4%); BNCs amendment also improved photosynthesis efficiency by promoting production of total chlorophyll (43.2%) and carotenoids contents (60.0%), and the nutritional profile of rice plants as compared with untreated diseased control. Moreover, BNCs-induced disease resistance response corresponded to an increase in transcript level of defense-related genes, such as OsPRs, OsSOD and OsAPX, in rice plants. High-throughput sequencing results revealed that BNCs amendment decreased the relative abundance of Xanthomonas (87.5%) by reshaping the phyllospheric and root-endophytic bacterial community of rice. In addition, BNCs increased the bacterial community diversity in healthy and diseased plants; significant increases in relative abundance of Ochrobactrum, Allorhizobium, Methylobacterium, Devosia, Pseudolabrys, Sphingomonas and Bradyrhizobium in BNCs-treated diseased and healthy plants was noted. These findings demonstrate that BNCs have potential to serve as non-toxic, sustainable and highly efficient alternative for plant disease management.
AB - Rice production is severely affected by the bacterial pathogen, Xanthomonas oryzae pv. oryzae (Xoo), which causes bacterial leaf blight (BLB) disease. Nano-enabled strategies have recently attracted attention as a sustainable platform to improve crop nutrition and protection. Here, we report the bioengineering of chitosan-iron nanocomposites (BNCs) along with their in vitro and in vivo bactericidal activity against Xoo. In addition, the impact of BNCs on the endophytic microbiome of healthy and BLB diseased rice was evaluated using a high-throughput sequencing technique. The BNCs were spherically shaped with an average size of 86 nm. In vitro antibacterial assays showed that BNCs significantly inhibited biological functions of the pathogen (viz., growth, motility, and biofilm formation) at 250 μg mL−1 concentration as compared with respective control. A greenhouse experiment demonstrated that foliar exposure to 250 mg·L−1 BNCs significantly reduced the BLB disease incidence (67.1%) through modulation of antioxidant enzymes viz., superoxide dismutase (49.2%), peroxidase (38.8%) and ascorbate peroxidase (53.4%); BNCs amendment also improved photosynthesis efficiency by promoting production of total chlorophyll (43.2%) and carotenoids contents (60.0%), and the nutritional profile of rice plants as compared with untreated diseased control. Moreover, BNCs-induced disease resistance response corresponded to an increase in transcript level of defense-related genes, such as OsPRs, OsSOD and OsAPX, in rice plants. High-throughput sequencing results revealed that BNCs amendment decreased the relative abundance of Xanthomonas (87.5%) by reshaping the phyllospheric and root-endophytic bacterial community of rice. In addition, BNCs increased the bacterial community diversity in healthy and diseased plants; significant increases in relative abundance of Ochrobactrum, Allorhizobium, Methylobacterium, Devosia, Pseudolabrys, Sphingomonas and Bradyrhizobium in BNCs-treated diseased and healthy plants was noted. These findings demonstrate that BNCs have potential to serve as non-toxic, sustainable and highly efficient alternative for plant disease management.
KW - Bactericidal
KW - BLB
KW - Nanocomposites
KW - Polymer
KW - Rice microbiome
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UR - http://www.scopus.com/inward/citedby.url?scp=85145737266&partnerID=8YFLogxK
U2 - 10.1016/j.nantod.2022.101547
DO - 10.1016/j.nantod.2022.101547
M3 - Article
AN - SCOPUS:85145737266
SN - 1748-0132
VL - 45
JO - Nano Today
JF - Nano Today
M1 - 101547
ER -