Biocorrosion and uptake of titanium by human osteoclasts

Dieter Cadosch, Mohamed S. Al-Mushaiqri, Oliver P. Gautschi, James Meagher, Hans Peter Simmen, Luis Filgueira

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

All metals in contact with a biological system undergo corrosion through an electrochemical redox reaction. This study investigated whether human osteoclasts (OC) are able to grow on titanium and aluminum, and directly corrode the metals leading to the release of corresponding metal ions, which are believed to cause inflammatory reactions and activate osteoclastic differentiation. Scanning electron microscopy analysis demonstrated long-term viable OC cultures on the surface of titanium and aluminum foils. Atomic emission spectrometry investigations showed significantly increased levels of aluminum in the supernatant of OC cultured on aluminum; however, all measurements in the supernatants of cell cultures on titanium were below detection limits. Despite this, confocal microscopy analysis with Newport Green DCF diacetate ester staining depicted intense fluorescence throughout the cytoplasm and nucleolus of OC cultured on titanium foils. Comparable fluorescence intensities were not observed in monocytes and control cells cultured on glass. The present study demonstrated that human osteoclast precursors are able to grow and differentiate toward mature OC on titanium and aluminum. Furthermore, it established that the mature cells are able to directly corrode the metal surface and take up corresponding metal ions, which subsequently may be released and thereby induce the formation of osteolytic lesions in the periprosthetic bone, contributing to the loosening of the implant.

Original languageEnglish
Pages (from-to)1004-1010
Number of pages7
JournalJournal of Biomedical Materials Research - Part A
Volume95
Issue number4
DOIs
Publication statusPublished - Dec 15 2010

Fingerprint

Titanium
Aluminum
Metals
Metal ions
Fluorescence
Aluminum foil
Confocal microscopy
Redox reactions
Biological systems
Cell culture
Metal foil
Spectrometry
Osteoclasts
Esters
Bone
Corrosion
Glass
Scanning electron microscopy

Keywords

  • aluminum
  • biocorrosion
  • Newport Green
  • osteoclast
  • osteolysis
  • titanium

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

Cite this

Biocorrosion and uptake of titanium by human osteoclasts. / Cadosch, Dieter; Al-Mushaiqri, Mohamed S.; Gautschi, Oliver P.; Meagher, James; Simmen, Hans Peter; Filgueira, Luis.

In: Journal of Biomedical Materials Research - Part A, Vol. 95, No. 4, 15.12.2010, p. 1004-1010.

Research output: Contribution to journalArticle

Cadosch, D, Al-Mushaiqri, MS, Gautschi, OP, Meagher, J, Simmen, HP & Filgueira, L 2010, 'Biocorrosion and uptake of titanium by human osteoclasts', Journal of Biomedical Materials Research - Part A, vol. 95, no. 4, pp. 1004-1010. https://doi.org/10.1002/jbm.a.32914
Cadosch, Dieter ; Al-Mushaiqri, Mohamed S. ; Gautschi, Oliver P. ; Meagher, James ; Simmen, Hans Peter ; Filgueira, Luis. / Biocorrosion and uptake of titanium by human osteoclasts. In: Journal of Biomedical Materials Research - Part A. 2010 ; Vol. 95, No. 4. pp. 1004-1010.
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