Biochemical profiling of mucilage extracted from seeds of different citrus rootstocks

Summar A. Naqvi, M. M. Khan, M. Shahid, M. J. Jaskani, Iqrar A. Khan, Mohammad Zuber, Khalid Mahmood Zia

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Hetero-polysaccharide mucilage was extracted from the seed coats of different citrus rootstocks viz. Rough lemon, Sachtion citrumelo and Yuma citrange for investigating its biochemical and molecular properties. Investigations showed that the mucilage contained (mg/g) starch 3.13-5.04; maltose 3.23-4.31; glucosamine 0.017-0.289; d-xylose 0.059-0.107 and total soluble sugars 8.13-11.82. Specific enzyme activities were 16.98-35.96, 30.60-98.45, 42.00-73.98, 660.98-738.35 and 7.660-19.27 IU mg-1 of protein for protease, amylase, catalase, peroxidase and superoxide dismutase, respectively. Proximate analysis showed 12.85-13.94% moisture, 11.25-14.06% crude protein, 0.31-0.86% crude lipid, 1.31-2.69% crude fibre, 2.95-3.45% ash and 81.48-91.49 kJ 100 g-1 energy. The comparative characterization of the extractable proteins was profiled by SDS-PAGE and quantified using Bradford assay. Structural properties of samples were analyzed and compared using Fourier transformation infrared (FT-IR) spectroscopy.

Original languageEnglish
Pages (from-to)623-628
Number of pages6
JournalCarbohydrate Polymers
Volume83
Issue number2
DOIs
Publication statusPublished - Jan 10 2011

Fingerprint

Seed
Ashes
Proteins
Glucosamine
Maltose
Amylases
Xylose
Enzyme activity
Polysaccharides
Starch
Sugars
Catalase
Lipids
Peroxidase
Superoxide Dismutase
Structural properties
Infrared spectroscopy
Assays
Peptide Hydrolases
Moisture

Keywords

  • Citrus
  • Mucilage
  • Polysaccharides
  • Rootstocks
  • Seed

ASJC Scopus subject areas

  • Organic Chemistry
  • Materials Chemistry
  • Polymers and Plastics

Cite this

Biochemical profiling of mucilage extracted from seeds of different citrus rootstocks. / Naqvi, Summar A.; Khan, M. M.; Shahid, M.; Jaskani, M. J.; Khan, Iqrar A.; Zuber, Mohammad; Zia, Khalid Mahmood.

In: Carbohydrate Polymers, Vol. 83, No. 2, 10.01.2011, p. 623-628.

Research output: Contribution to journalArticle

Naqvi, Summar A. ; Khan, M. M. ; Shahid, M. ; Jaskani, M. J. ; Khan, Iqrar A. ; Zuber, Mohammad ; Zia, Khalid Mahmood. / Biochemical profiling of mucilage extracted from seeds of different citrus rootstocks. In: Carbohydrate Polymers. 2011 ; Vol. 83, No. 2. pp. 623-628.
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