Barkedji virus, a novel mosquito-borne flavivirus identified in Culex perexiguus mosquitoes, Israel, 2011

Jolanta Kolodziejek, Karin Pachler, Hanna Bin, Ella Mendelson, Lester Shulman, Laor Orshan, Norbert Nowotny

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Barkedji virus, named after the area of its first identification in Senegal, is a newly discovered flavivirus (FV), for which we propose the abbreviation BJV. In the present study, we report the firsttime detection of BJV in Culex perexiguus mosquitoes in Israel in 2011 and determination of its almost complete polyprotein gene sequence. We characterized the BJV genome and defined putative mature proteins, conserved structural elements and potential enzyme motifs along the polyprotein precursor. By comparing polyproteins and individual proteins of BJV with several other FVs, a distant relationship of BJV to Nounane virus (NOUV), a recently described African FV, is demonstrated. Phylogenetic analysis of 55 selected flaviviral polyprotein gene sequences exhibits two major clusters, one made up of the classical three clades of FVs: mosquito-borne, tick-borne and those without known vectors. The other cluster exclusively contains so-called 'insect-specific' FVs, which do not replicate in vertebrate cells. Based on our phylogenetic analysis, BJV is related to other members of the mosquito-borne clade with yet unknown vertebrate hosts, such as NOUV, Donggang virus, Chaoyang virus and Lammi virus. However, with a maximum identity of only 54% to NOUV, BJV represents a distinct new virus species.

Original languageEnglish
Pages (from-to)2449-2457
Number of pages9
JournalJournal of General Virology
Volume94
Issue numberPART 11
DOIs
Publication statusPublished - Nov 2013

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Flavivirus
Culex
Israel
Culicidae
Viruses
Polyproteins
Vertebrates
Senegal
Ticks
Genes
Insects
Genome
Enzymes

ASJC Scopus subject areas

  • Virology

Cite this

Barkedji virus, a novel mosquito-borne flavivirus identified in Culex perexiguus mosquitoes, Israel, 2011. / Kolodziejek, Jolanta; Pachler, Karin; Bin, Hanna; Mendelson, Ella; Shulman, Lester; Orshan, Laor; Nowotny, Norbert.

In: Journal of General Virology, Vol. 94, No. PART 11, 11.2013, p. 2449-2457.

Research output: Contribution to journalArticle

Kolodziejek, Jolanta ; Pachler, Karin ; Bin, Hanna ; Mendelson, Ella ; Shulman, Lester ; Orshan, Laor ; Nowotny, Norbert. / Barkedji virus, a novel mosquito-borne flavivirus identified in Culex perexiguus mosquitoes, Israel, 2011. In: Journal of General Virology. 2013 ; Vol. 94, No. PART 11. pp. 2449-2457.
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