Bacterially produced rice thaumatin-like protein shows in vitro antifungal activity

J. Jayaraj, R. Velazhahan, D. Fu, G. H. Liang, S. Muthukrishnan

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Thaumatin-like proteins (TLPs) are pathogenesis-related (PR) proteins of group 5 believed to be involved in plant defense mechanisms. A full-length TLP cDNA clone was previously isolated from a rice cDNA library prepared from RNA isolated from Rhizoctonia solani-infected rice plants. The cDNA region corresponding to the putative mature TLP was expressed in Escherichia coli as a Histagged fusion protein and purified by Ni-NTA resin column chromatography. The purified 23-kDa rice TLP inhibited the mycelial growth of Fusarium oxysporum f. sp. cubense, F. graminearum, Botrytis cinerea, Curvularia lunata, Drechslera oryzae, Sarocladium oryzae and Rhizoctonia solani in vitro at a concentration of 5 μg per disc. The fungi Aspergillus niger, Alternaria alternata and Sclerotium oryzae were inhibited only at 10 μg level. TLP had no effect on the growth of Macrophomina phaseolina and Sclerotinia sclerotiorum even at 10 μg concentration. The purified TLP also inhibited spore germination and germ tube growth of some fungi. The sensitive fungi exhibited growth abnormalities including swelling of hyphal tips and excessive branching of hyphae.

Original languageEnglish
Pages (from-to)334-344
Number of pages11
JournalZeitschrift fur Pflanzenkrankheiten und Pflanzenschutz
Volume111
Issue number4
Publication statusPublished - Jul 2004

Fingerprint

rice
proteins
Thanatephorus cucumeris
fungi
Magnaporthe salvinii
Sarocladium oryzae
Fusarium oxysporum f. sp. cubense
Macrophomina phaseolina
pathogenesis-related proteins
Sclerotinia sclerotiorum
germ tube
Alternaria alternata
Fusarium graminearum
spore germination
Botrytis cinerea
Aspergillus niger
defense mechanisms
cDNA libraries
hyphae
resins

Keywords

  • Escherichia coli
  • In vitro antifungal activity
  • Rice, thaumatin-like protein

ASJC Scopus subject areas

  • Plant Science

Cite this

Bacterially produced rice thaumatin-like protein shows in vitro antifungal activity. / Jayaraj, J.; Velazhahan, R.; Fu, D.; Liang, G. H.; Muthukrishnan, S.

In: Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz, Vol. 111, No. 4, 07.2004, p. 334-344.

Research output: Contribution to journalArticle

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